FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688982

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688982
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences919838
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTAGCCCACCGCTCACTTTCGGCGGAGGGA40720.4426866469965363No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32010.3479960601758136No Hit
GTAATAGACAGCCGTGTCGTCAGATCTCAGTCTGTTCATTTGCAGAAACA30710.33386313676973556No Hit
GAGTATTACCAGCAGCTACTTAGCCTGGTACCAGCAAAAACCTGGCCAGG27770.30190098691291295No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27510.29907440223169734No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA26660.2898336446200309No Hit
GTCTATTACTGTGCGAGAGATGGCACAATGGGAGCTACTAGGATGGAGGG23790.25863249833122787No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23630.256893061604326No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA20840.22656163367897392No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20490.222756615838876No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19380.21068927354599398No Hit
GTGTATTACTGTCAGCAGTATGGTAGCCCACCGCTCACTTTCGGCGGAGGGACCAAGGTAGAGATCAAACGAA19340.2102544143642685No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17570.19101189557291612No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17560.19090318077748472No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17350.188620170073426No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16780.1824234267338379No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT16220.17633539818968122No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16130.1753569650307989No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14990.16296347835162278No Hit
CTCCTGCAGGGCCAGTCAGAGTATTACCAGCAGCTACTTAGCCTGGTACC14570.15839745694350527No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14560.1582887421480739No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA14500.15763645337548568No Hit
GTATGGTAGCCCACCGCTCACTTTCGGCGGAGGGACCAAGGTAGAGATCAAACGAACTGTGGCTGCACCAT14420.15676673501203472No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14050.15274428758107406No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13870.1507874212633094No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13650.14839569576381928No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13520.14698240342321148No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTACCAG13470.14643882944605463No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13000.14132923406078027No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12610.13708935703895686No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT12570.13665449785723138No Hit
GTCTTTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAGTCAGA12500.1358934942892118No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11520.1252394443369376No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGC11160.1213257117014083No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTAGCCCA10780.11719454947501624No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10640.11567254233897709No Hit
CTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC10530.11447667958923201No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCTACCTTGGTCC10450.11360696122578107No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10430.11338953163491831No Hit
GTGCAGCCACAGTTCGTTTGATCTCTACCTTGGTCCCTCCGCCGAAAGTG10320.11219366888517326No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10050.10925836940852629No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC10010.1088235102268008No Hit
CTCCTGTGCAGCGTCTGGATTCAGCTTCAGTAGCTATGGCATGCACTGGGTCCGCCAGGCTCCGGGCAAGGGG9900.10762764747705575No Hit
GACCAAGGTAGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT9890.10751893268162438No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9800.10654049952274205No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA9710.10556206636385972No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGCAGATGCCACCGG9570.10404005922782056No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACC9420.10240933729635002No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9310.10121347454660494No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT9270.10077861536487948No Hit
ACCTTGGTCCCTCCGCCGAAAGTGAGCGGTGGGCTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGG9270.10077861536487948No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGGAG8400.049.48907570
CTAACGC452.1667329E-644.9948543
GGTATCA11200.040.399011
GTATAAC451.140512E-437.4957123
GTATCAA27700.037.179541
CCTAACG558.580042E-636.8279842
GATTCAC2500.035.116991
CTCTGGA8850.035.1149469
ATTCACT2600.033.7589872
CTAAGGT400.00289228133.7479744
AGGCGGT751.6911999E-632.14481439
TATCAAC32050.031.8103432
GGTGTTC7300.031.50929852
ACGGTTA450.004172791731.31438850
GAATTGT8100.031.04577362
CAGGGAA9400.030.88679970
ATTGTCT8250.030.68672464
TCAACGC33450.030.6708054
ATCAACG33750.030.3965243
CAACGCA34100.030.378125
Produced by FastQC (version 0.11.5)