FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688983

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688983
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1270208
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT55300.4353617675215398No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC53190.41875031490905423No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC38800.3054617826371744No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG35810.28192233083085605No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32600.2566508792260795No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32330.25452524310978986No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31130.24507797148183605No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30560.24059051745855797No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG30530.24035433566785913No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA30150.2373626996523404No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCACGACATTAGAAA29750.2342136091096891No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28430.22382161031893988No Hit
ATATTACTGTCAACAATATGATAATGTCCCTCACACTTTTGGCCAGGGGA26940.21209124804756388No Hit
CTTTTGGCCAGGGGACCAAGCTGGACATCAAACGAACTGTGGCTGCACCA26870.2115401572025999No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26450.20823361213281605No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26230.20650161233435782No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG26230.20650161233435782No Hit
GTCTCTGGTGCCTCCATCAGTAATTACTACTGGAGCTGGATCCGGCAGCC26080.2053207033808636No Hit
AAGCAGGGAAGGCCCCTAAGCTCCTGATCTACGATGCATCCAATTTGGAA25600.20154179472968206No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25080.19744797702423542No Hit
GCACAAAGCAGGGAAGGCCCCTAAGCTCCTGATCTACGATGCATCCAATTTGGAAGAAGGGGTCCCGTCAAGGTT24470.1926456139466922No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23800.1873708872877513No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23140.18217488789237668No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA23010.18115143346601503No Hit
ACATATTACTGTCAACAATATGATAATGTCCCTCACACTTTTGGCCAGGGGACCAAGCTGGACATCAAACGAA21850.1720190708923263No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21660.17052325288456693No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21280.16753161686904822No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG21070.1658783443341563No Hit
CAGTAATATGTTGCAAAATCTTCAGGCTGCAGGTTGGTGATGGTGAAAGTAAAATCTGTCCCAGATCCACTTCCA20530.16162707210157706No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20420.16076107220234795No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20360.16028870862095027No Hit
GTAATATGTTGCAAAATCTTCAGGCTGCAGGTTGGTGATGGTGAAAGTAAAATCTGTCCCAGATCCACTTCCA20100.15824179976822694No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT19660.15477780017131051No Hit
GTGCAGCCACAGTTCGTTTGATGTCCAGCTTGGTCCCCTGGCCAAAAGTG19400.1527308913185872No Hit
ATATGATAATGTCCCTCACACTTTTGGCCAGGGGACCAAGCTGGACATCAAACGAACTGTGGCTGCACCAT19280.1517861641557918No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG19040.14989670983020104No Hit
GTCTCCATCCTCCCTGTCAGCGTCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCACGACATTAGAAA18900.14879452814027308No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18760.14769234645034512No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT18710.14729871013251372No Hit
TCCCTGCTTTGTGCTGATACCAATTTAAAAAGTTTCTAATGTCGTGACTCGCCTGGCAAGTGATGGTGACT18620.14659016476041717No Hit
GGCCAAAAGTGTGAGGGACATTATCATATTGTTGACAGTAATATGTTGCA18420.14501561948909156No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC18230.1435198014813322No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18110.1425750743185368No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17480.13761525671386104No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16990.1337576207991132No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC16340.1286403486673049No Hit
GATCTACGATGCATCCAATTTGGAAGAAGGGGTCCCGTCAAGGTTCAGTGGAAGTGGATCTGGGACAGATTTTA16340.1286403486673049No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC16290.12824671234947346No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15950.12556998538821987No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGTCCCAGTCTCCATCCTCC15880.1250188945432559No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT15860.12486144001612334No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG15780.12423162190759308No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT15440.12155489494633949No Hit
CCCTGAAGGTGACCTCTGTGACCACTGCGGACACGGCCCTTTATTTCTGT15290.12037398599284525No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15060.11856325893082077No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15030.11832707714012193No Hit
GCTTTGTGCTGATACCAATTTAAAAAGTTTCTAATGTCGTGACTCGCCTGGCAAGTGATGGTGACT15010.11816962261298938No Hit
TTATCATATTGTTGACAGTAATATGTTGCAAAATCTTCAGGCTGCAGGTTGGTGATGGTGAAAGTAAAATCTGTC14980.11793344082229053No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA14940.1176185317680254No Hit
GTTCAGTGGAAGTGGATCTGGGACAGATTTTACTTTCACCATCACCAACC14840.11683125913236259No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG14760.11620144102383231No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGTCCAGCTTGGTCC14710.1158078047060009No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14650.11533544112460321No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14550.1145481684889404No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14460.11383962311684387No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14400.11336725953544616No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14330.11281616869048218No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14240.11210762331838565No Hit
CAATATGATAATGTCCCTCACACTTTTGGCCAGGGGACCAAGCTGGACATCAAACGAACTGTGGCTGCACCAT14230.11202889605481936No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14210.1118714415276868No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14190.11171398700055425No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT14180.11163525973698796No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA14120.11116289615559027No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13990.11013944172922858No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC13950.10982453267496348No Hit
CTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCACGACATTAGA13860.10911598730286692No Hit
GATTTTACTTTCACCATCACCAACCTGCAGCCTGAAGATTTTGCAACATATTACTGTCAACAATATGATAATGTC13850.10903726003930064No Hit
CAGCTGCACTGTCTCTGGTGCCTCCATCAGTAATTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGA13810.10872235098503553No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13780.10848616919433667No Hit
GCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGTCCCAGTCTCCATC13630.10730526024084244No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG13590.10699035118657732No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13560.10675416939587848No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13510.10636053307804705No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT13420.10565198770595052No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC13370.1052583513881191No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12960.10203053358190153No Hit
GAAATAAAGGGCCGTGTCCGCAGTGGTCACAGAGGTCACCTTCAGGGAGA12920.10171562452763643No Hit
ATCATATTGTTGACAGTAATATGTTGCAAAATCTTCAGGCTGCAGGTTGG12840.10108580641910617No Hit
TCACCAACCTGCAGCCTGAAGATTTTGCAACATATTACTGTCAACAATAT12830.10100707915553987No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA12790.10069217010127475No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)