FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688984

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688984
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1270208
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT53390.4203248601803799No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC42630.3356143245830604No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC39420.3103428729782839No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC35250.2775136040711443No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG33630.26475978737340655No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31510.24806960749735474No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31260.24610142590819772No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA29010.22838779160578426No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27660.21775961102433616No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT27300.21492542953595No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCACGACATTAGAAA27190.2140594296367209No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27010.21264233889252784No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT26270.20681652138862297No Hit
ATATTACTGTCAACAATATGATAATGTCCCTCACACTTTTGGCCAGGGGA26170.20602924875296014No Hit
CTTTTGGCCAGGGGACCAAGCTGGACATCAAACGAACTGTGGCTGCACCA25020.19697561344283768No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24400.19209452310172823No Hit
GTCTCTGGTGCCTCCATCAGTAATTACTACTGGAGCTGGATCCGGCAGCC24280.19114979593893286No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24280.19114979593893286No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24170.19028379603970375No Hit
AAGCAGGGAAGGCCCCTAAGCTCCTGATCTACGATGCATCCAATTTGGAA23600.18579634201642564No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22650.17831725197762885No Hit
GCACAAAGCAGGGAAGGCCCCTAAGCTCCTGATCTACGATGCATCCAATTTGGAAGAAGGGGTCCCGTCAAGGTT22100.17398725248148333No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22080.1738297979543508No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG22020.1733574343729531No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT21930.17264888900085656No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21830.17186161636519373No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21410.1685550712954099No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21110.16619325338842142No Hit
ACATATTACTGTCAACAATATGATAATGTCCCTCACACTTTTGGCCAGGGGACCAAGCTGGACATCAAACGAA20860.16422507179926438No Hit
GTAATATGTTGCAAAATCTTCAGGCTGCAGGTTGGTGATGGTGAAAGTAAAATCTGTCCCAGATCCACTTCCA20200.15902907240388975No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT19740.1554076182798408No Hit
GTGCAGCCACAGTTCGTTTGATGTCCAGCTTGGTCCCCTGGCCAAAAGTG19720.1552501637527082No Hit
CAGTAATATGTTGCAAAATCTTCAGGCTGCAGGTTGGTGATGGTGAAAGTAAAATCTGTCCCAGATCCACTTCCA19470.15328198216355118No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC18860.14847961908600796No Hit
GGCCAAAAGTGTGAGGGACATTATCATATTGTTGACAGTAATATGTTGCA18720.14737743739608003No Hit
TCCCTGCTTTGTGCTGATACCAATTTAAAAAGTTTCTAATGTCGTGACTCGCCTGGCAAGTGATGGTGACT18420.14501561948909156No Hit
GTCTCCATCCTCCCTGTCAGCGTCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCACGACATTAGAAA18190.14320489242706708No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA18190.14320489242706708No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17770.1398983473572832No Hit
ATATGATAATGTCCCTCACACTTTTGGCCAGGGGACCAAGCTGGACATCAAACGAACTGTGGCTGCACCAT17750.13974089283015065No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17410.13706416586889708No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16480.12974253035723282No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16350.12871907593087117No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC16130.12698707613241295No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16040.12627853076031642No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15880.1250188945432559No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA15820.12454653096185822No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15640.12312944021766513No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15630.12305071295409886No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG15580.12265707663626745No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15450.12163362220990578No Hit
TTATCATATTGTTGACAGTAATATGTTGCAAAATCTTCAGGCTGCAGGTTGGTGATGGTGAAAGTAAAATCTGTC15340.12076762231067667No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT15280.12029525872927899No Hit
CCCTGAAGGTGACCTCTGTGACCACTGCGGACACGGCCCTTTATTTCTGT15110.11895689524865219No Hit
GCTTTGTGCTGATACCAATTTAAAAAGTTTCTAATGTCGTGACTCGCCTGGCAAGTGATGGTGACT15090.11879944072151961No Hit
GATCTACGATGCATCCAATTTGGAAGAAGGGGTCCCGTCAAGGTTCAGTGGAAGTGGATCTGGGACAGATTTTA14660.1154141683881695No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14520.11431198669824155No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14470.11391835038041014No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14350.11297362321761475No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14240.11210762331838565No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC14150.11139907794628912No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC14100.1110054416284577No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14080.11084798710132514No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGTCCCAGTCTCCATCCTCC14000.11021816899279488No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13850.10903726003930064No Hit
GATTTTACTTTCACCATCACCAACCTGCAGCCTGAAGATTTTGCAACATATTACTGTCAACAATATGATAATGTC13840.10895853277573438No Hit
GTTCAGTGGAAGTGGATCTGGGACAGATTTTACTTTCACCATCACCAACC13820.1088010782486018No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13740.10817126014007156No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13610.10714780571370987No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT13520.10643926034161334No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13380.10533707865168539No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13340.10502216959742026No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA13250.10431362422532373No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT13120.10329016979896206No Hit
GAAATAAAGGGCCGTGTCCGCAGTGGTCACAGAGGTCACCTTCAGGGAGA12740.10029853378344335No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)