FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688985

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688985
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences606802
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGAGAGCAAGCAGTAATACTCAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTGGCCC26770.44116532246103346No Hit
CTCCTATACTGGTCCTCGACGGGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTCAGCCCAAGGCTGCCCC25670.4230374982284172No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT18530.30537143911852627No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC18050.297461115817021No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16710.2753781299336522No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14220.23434332780709358No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA13220.2178634875956243No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG12850.2117659467173806No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12480.20566840583913698No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC12140.2000652601672374No Hit
CTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCCAAGCTGCCCTGACCCTTTCGGGTGCGCAGC11920.1964396953207142No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT11630.19166054165938806No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC11590.1910013480509293No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG11400.18787017841075013No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT11190.18440941196634159No Hit
GTATTACTGCTTGCTCTCCTATACTGGTCCTCGACGGGTCTTCGGCGGAG10400.17139033819928082No Hit
GAGTATTACTGCTTGCTCTCCTATACTGGTCCTCGACGGGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTAAGT10220.16842396696121634No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT9810.16166723247451392No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG9510.15672328041107314No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC9500.15655848200895844No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT9410.1550752963899262No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT9210.15177932834763236No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA9020.14864815870745318No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG8840.1456817874693887No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCCGTCGAGGACCAGTATAGGAGAGCAAGCAGTAATACT8750.14419860185035646No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTC8700.14337460983978298No Hit
GTCCAGGAGTGTTTGTTACTTGTATCAAAAATCAGTGTCCTGGGGGCTTG8610.14189142422075077No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC8390.1382658593742275No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC8160.13447549612558957No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCCGTCGAGGACCAGTATAGGAGAGCAAG8130.1339811009192455No Hit
GCCTGAGGATGAGGCTGAGTATTACTGCTTGCTCTCCTATACTGGTCCTC8000.13183872169175448No Hit
TCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTGGCCCCCAAGGAGGGAGCCTGAGAACCGGGCAGGGGTC7930.13068513287695163No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC7760.12788356004100185No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG7750.12771876163888715No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC7680.1265651728240843No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7650.12607077761774022No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA7520.12392839839024922No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA7440.12261001117333166No Hit
GTAATACTCAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAG7300.12030283354372596No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG7260.1196436399352672No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA7180.11832525271834964No Hit
CAGTAATACTCAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTGGCCCCCAAGGAGGGAGC7170.11816045431623495No Hit
CGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCAT7140.11766605910989086No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCATCCCTCACCCCCCAC7100.1170068655014321No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT7070.11651247029508803No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG7020.11568847828451455No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG6740.11107412302530315No Hit
CCTATACTGGTCCTCGACGGGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTCAGCCCAAGGCTGCCCCCT6680.110085332612615No Hit
GTATCAACGCAGAGTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCC6490.10695416297243582No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC6310.10398779173437134No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT6310.10398779173437134No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA6230.10266940451745381No Hit
GGCCTGGCCAAGCCCCCAGGACACTGATTTTTGATACAAGTAACAAACAC6220.10250460611533911No Hit
GAATATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC6160.10151581570265093No Hit
GGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGG6090.10036222688784809No Hit
CATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCATCCCTCACCCCCCACCA6090.10036222688784809No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG3200.063.7568370
ATCACTA400.00886515749.36012670
TCTCGTA2650.046.63502538
TCGTATG2850.045.9805440
GGTATCA11750.045.7511021
CGTATGC2950.045.73016741
GCCGTCT3200.043.8563146
GTATGCC3250.043.7750442
CCCACGA3150.043.7241319
CCTAACG705.7480065E-1043.190912
ATGCCGT3250.042.96707544
TATACAC3200.041.991163
CCGTCTT3400.041.37625547
CACGAGA3350.041.1137321
ATCTCCG3350.041.1035710
TCTCCGA3350.041.1035711
CTAACGC751.1295924E-940.3115123
CTACATC3200.040.3001533
CGTCTTC3500.040.28445448
ATCTCGT3100.039.7649537
Produced by FastQC (version 0.11.5)