FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688986

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688986
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences606802
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37420.6166756207131816No Hit
CTCCTATACTGGTCCTCGACGGGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTCAGCCCAAGGCTGCCCC26990.4447908873075566No Hit
GTATAGGAGAGCAAGCAGTAATACTCAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTGGCCC25130.41413838451422375No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18150.29910909983816797No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC16630.2740597427167346No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15270.25164716002913634No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG13590.22396102847386792No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA13230.21802828599773896No Hit
CTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCCAAGCTGCCCTGACCCTTTCGGGTGCGCAGC12290.2025372361989578No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12100.19940606655877866No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG11950.19693409052705824No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT11090.18276142794519465No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC11030.1817726375325065No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT10820.17831187108809793No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT10490.17287352381831306No Hit
GTATTACTGCTTGCTCTCCTATACTGGTCCTCGACGGGTCTTCGGCGGAG10410.1715551366013955No Hit
GAGTATTACTGCTTGCTCTCCTATACTGGTCCTCGACGGGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTAAGT10400.17139033819928082No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT9410.1550752963899262No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC8950.1474945698926503No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG8940.1473297714905356No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC8780.14469299705670055No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC8730.14386900504612707No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG8370.13793626256999814No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCCGTCGAGGACCAGTATAGGAGAGCAAGCAGTAATACT8310.13694747215730996No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA8220.13546428653827772No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA8060.13282751210444263No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT8000.13183872169175448No Hit
GTCCAGGAGTGTTTGTTACTTGTATCAAAAATCAGTGTCCTGGGGGCTTG7880.12986114086637815No Hit
GCCTGAGGATGAGGCTGAGTATTACTGCTTGCTCTCCTATACTGGTCCTC7850.12936674566003406No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC7850.12936674566003406No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC7730.12738916483465776No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA7650.12607077761774022No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCCGTCGAGGACCAGTATAGGAGAGCAAG7630.12574118081351082No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTC7410.12211561596698758No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC7320.12063243034795534No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG7310.12046763194584066No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA7280.11997323673949659No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT7260.1196436399352672No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG7020.11568847828451455No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA7020.11568847828451455No Hit
GTAATACTCAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAG6700.11041492941684437No Hit
GTATCAACGCAGAGTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCC6680.110085332612615No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT6660.1097557358083856No Hit
CAGTAATACTCAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTGGCCCCCAAGGAGGGAGC6590.10860214699358274No Hit
TCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTGGCCCCCAAGGAGGGAGCCTGAGAACCGGGCAGGGGTC6450.10629496936397705No Hit
CGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCAT6440.10613017096186236No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG6200.10217500931110973No Hit
CCTATACTGGTCCTCGACGGGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTCAGCCCAAGGCTGCCCCCT6200.10217500931110973No Hit
GAATATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC6080.1001974284857334No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCAT401.03304716E-466.34141570
AGTGCGT200.00817049250.395752
TACGTAT1451.8189894E-1145.752770
CCTAACG350.001534856438.3967632
CTAACGC350.00153547838.3935973
GGTATCA14100.037.654091
TGGACGA400.00252871934.68334242
CTAGTGG400.002955684233.5971644
GCTATAT502.1760911E-433.5916313
ATGGCAA4650.032.81403470
GTATCAA37750.032.2229921
GTGGTAT4100.031.9635491
TATCAAC39250.030.7296372
TTCCGTG450.004952362730.23542234
ACGCACA450.00526293929.8616857
GGCTACG450.00526293929.86168510
AACGCAG40450.029.7324926
ATCAACG40600.029.705393
TCAACGC40850.029.5260264
CAACGCA41550.029.0262055
Produced by FastQC (version 0.11.5)