FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688987

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688987
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1001068
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC40220.4017709086695409No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG32230.32195615083091256No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT30280.30247695461247387No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG28380.28349722496373875No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG25330.2530297642118218No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT24000.2397439534577072No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC23920.2389448069461815No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCGC22850.22825622235452536No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT22110.2208641171229127No Hit
ACCTAATACTGGCTGGTCCTTTGACTACTGGGGCCCGGGAACACTGGTCACCGTGTCCTCAGCATCCCCGACCAG20700.2067791598572724No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC20080.20058577439294833No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC19770.1974890816607863No Hit
TCTCTGGGCTCCAGCCTGAGGATGAGGCTAATTATTACTGCAGCTCATTT19650.19629036189349772No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCGCCGCCGAAAACGGGATTGTTGCTGCCTACAAATGAGCTG19510.1948918554983278No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATCACCGCGTCTCCTGGTACCAACAACACCCAGGCAAAGC19340.19319366916133568No Hit
GTATTAGGTTGATCTTTCGCACAATAATACACGGCCGTGTCCTCGGCTCT19320.19299388253345426No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19300.19279409590557284No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19010.18989718980129222No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17460.17441372614048198No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17320.173015219745312No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT17250.17231596654772702No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG17200.17181649997802345No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG16560.16542332788581796No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG16490.16472407468823297No Hit
GACCTAGGACGGTCAGCTTGGTCCCGCCGCCGAAAACGGGATTGTTGCTG16140.16122780870030806No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATCACCGCGTCTCCTGGTACCAACAACA16060.16042866218878238No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15610.15593346306145037No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT15540.15523420986386538No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15500.15483463660810254No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15430.15413538341051755No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG15090.15073901073653337No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT14580.14564445172555712No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14080.14064978602852152No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC14000.13985063951699586No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT13800.13785277323818163No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT13670.13655416015695238No Hit
AATTATTACTGCAGCTCATTTGTAGGCAGCAACAATCCCGTTTTCGGCGGCGGGACCAAGCTGACCGTCCTAGGT13400.13385704068055315No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC13270.13255842759932393No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12600.12586557556529626No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12430.12416738922830417No Hit
GACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCT12300.12286877614707493No Hit
CCCCTGAGGGCCGCTTACTGACCTCATAAATCATCAGTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGA12160.12147026975190497No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11920.11907283021732788No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11900.11887304358944646No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC11900.11887304358944646No Hit
GCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG11870.11857336364762434No Hit
GGTTATCACCGCGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAACTGATGATTTATGAGGTCAGTAAG11740.11727475056639508No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11680.11667539068275082No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC11610.11597613748516584No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT11590.11577635085728442No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT11520.11507709765969944No Hit
AGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCA11250.11237997818330024No Hit
GTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGAC11240.11228008486935952No Hit
GTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGACGCGGTGATAA11090.11078168516024886No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG10990.10978275202084174No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC10880.10868392556749391No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10770.1075850991141461No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCGTCTCTGGGCTCCAGC10720.10708563254444253No Hit
TAATTAGCCTCATCCTCAGGCTGGAGCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG10690.10678595260262039No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC10650.10638637934685755No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC10600.105886912777154No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10350.1033895799286362No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGG10350.1033895799286362No Hit
GCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCA10310.10299000667287336No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT10310.10299000667287336No Hit
CAATAATACACGGCCGTGTCCTCGGCTCTCAGGCTGCTCATTTGTAGATA10300.10289011335893267No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10140.10129182033588129No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC10130.10119192702194056No Hit
GTAATAATTAGCCTCATCCTCAGGCTGGAGCCCAGAGACGGTCAGGGAGG10130.10119192702194056No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG10130.10119192702194056No Hit
GCTTAGTACAGCCTGGGGGGTCCCTCAGACTCTCCTGTGAAGCCTCTGGA10070.10059256713829631No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG1800.051.61334640
CGTATGC1900.048.98610741
CGAGACG1950.048.77810723
TCTCGTA1800.047.66950238
CCCACGA2050.046.3894219
ATCTCGT1850.046.32501237
TATCTCG1850.046.28066636
CGGACTC2100.045.3710428
GACGGAC2100.045.3279326
AGGGGGG2400.044.43906470
GGTATCA8750.043.488721
CTCCTAT2200.043.415232
ATGCCGT2000.043.33131444
CCGTCTT2150.042.17860847
TCTCCGA2300.041.3450211
CCTATCT2200.040.37691534
GTATGCC2400.040.31306542
CGTCTTC2450.039.94101348
ACTCCTA2400.039.7693331
ACGAGAC2400.039.63023822
Produced by FastQC (version 0.11.5)