FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688988

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688988
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1001068
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC38720.38678691157843426No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCGCCGCCGAAAACGGGATTGTTGCTGCCTACAAATGAGCTG32870.32834932292311814No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG30510.30477450083311025No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG29570.2953845293226834No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT27960.2793017057782289No Hit
GACCTAGGACGGTCAGCTTGGTCCCGCCGCCGAAAACGGGATTGTTGCTG26080.2605217627573751No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG24580.24553776566626842No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG22780.2275569691569404No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCGC22390.22366112991325263No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC21990.21966539735562418No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT21480.2145708383446479No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT21130.21107457235672303No Hit
ACCTAATACTGGCTGGTCCTTTGACTACTGGGGCCCGGGAACACTGGTCACCGTGTCCTCAGCATCCCCGACCAG19900.19878769474201552No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19280.19259430927769142No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC18960.18939772323158865No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18710.18690039038307088No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG18670.18650081712730804No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC18020.1800077517211618No Hit
GTATTAGGTTGATCTTTCGCACAATAATACACGGCCGTGTCCTCGGCTCT17570.17551255259382978No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATCACCGCGTCTCCTGGTACCAACAACACCCAGGCAAAGC17190.17171660666408275No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16220.16202695521183375No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC16150.16132770201424879No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16090.16072834213060452No Hit
TCTCTGGGCTCCAGCCTGAGGATGAGGCTAATTATTACTGCAGCTCATTT15770.15753175608450173No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG15760.15743186277056104No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15500.15483463660810254No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15390.15373581015475474No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT15090.15073901073653337No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG14870.14854135782983774No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT14570.1455445584116164No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14250.14234797236551364No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14000.13985063951699586No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATCACCGCGTCTCCTGGTACCAACAACA14000.13985063951699586No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13320.13305789416902747No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC13290.13275821422720535No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC13250.1323586409714425No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT12690.12676461539076267No Hit
GTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGACGCGGTGATAA12280.12266898951919351No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12220.12206962963554922No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC12220.12206962963554922No Hit
ACCTAGGACGGTCAGCTTGGTCCCGCCGCCGAAAACGGGATTGTTGCTGC12000.1198719767288536No Hit
CCCCTGAGGGCCGCTTACTGACCTCATAAATCATCAGTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGA11930.1191727235312686No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11900.11887304358944646No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG11810.11797400376398007No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11710.11697507062457296No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC11490.1147774177178773No Hit
AATTATTACTGCAGCTCATTTGTAGGCAGCAACAATCCCGTTTTCGGCGGCGGGACCAAGCTGACCGTCCTAGGT11370.11357869795058878No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT11250.11237997818330024No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT11230.11218019155541882No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC11180.11168072498571525No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT11140.11128115172995241No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC11120.111081365102071No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA10990.10978275202084174No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC10810.10798467236990894No Hit
GTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGAC10770.1075850991141461No Hit
GGTTATCACCGCGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAACTGATGATTTATGAGGTCAGTAAG10680.10668605928867969No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT10630.10618659271897614No Hit
GCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG10500.10488797963774689No Hit
AGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCA10360.10348947324257693No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC10260.10249054010316981No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC10240.10229075347528839No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10040.10029288719647417No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9500.043.953591
CGCGCAA503.2011176E-642.5138454
TAACGCC350.001462176438.780154
GTGGTAT2250.037.7198831
GTATCAA23700.036.5262151
GCGCAAA601.1012269E-535.5115655
CCTAACG400.002813305233.9394152
GTCCTAT800.003079877733.3151670
ATCAACG27500.031.3429783
TCAACGC27500.031.3414134
AACGCAG28000.031.1453086
TATCAAC27850.030.9537222
CAACGCA28300.030.8136065
ATACGTA1450.030.4208411
GGCTATA450.004859265430.35492733
GTACGGA450.00500189330.1759071
TTAGGGC450.00501168130.1638473
CTAACGC450.00501168130.1638473
CGTACCA1501.8189894E-1229.40534214
TGCGGTC500.00580147429.2739865
Produced by FastQC (version 0.11.5)