FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688991

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688991
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences674433
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC21490.31863802631247284No Hit
GATTTATAGTACAAGCAACAAATACTCCTGGACACCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGC16710.24776367704427274No Hit
CACTAACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGCTTCCAGC14640.21707122872101453No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG14260.21143686622688984No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAAGACGCC14130.20950932116311033No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC12020.1782237820509969No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT11990.1777789639593555No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAAGACGCCCACCTGAGCA11910.17659278238164502No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA11050.16384133042125756No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG10850.1608758764769814No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA10730.1590966041104157No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG10580.15687251365220858No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT10090.14960715148873202No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT10030.14871751530544916No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9730.14426933438903494No Hit
CTATTACTGTTTACTCTACTTTGGTGCTGCTCAGGTGGGCGTCTTCGGCG9650.14308315281132447No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC9490.14071078965590356No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTC9490.14071078965590356No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG9170.1359660633450617No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA8890.13181442782307506No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG8820.13077651894257844No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG8750.1297386100620818No Hit
GACTATTACTGTTTACTCTACTTTGGTGCTGCTCAGGTGGGCGTCTTCGG8450.12529042914566754No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT8170.12113879362368092No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG8050.11935952125711523No Hit
CTATAAATCAGTGCCGTGGGTGCTTGTCCACGTTTCTGCTGGAACCAGTTTGGAAAGTAACCACTGGTGACTGCT7900.11713543079890812No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC7670.11372515876299054No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC7550.11194588639642485No Hit
GTGGTGACTCAGGAGCCCTCACTAACTGTGTCCCCAGGAGGGACAGTCAC7430.11016661402985917No Hit
GTAATAGTCAGCCTCGTCCTCAGGCTGCACACCCGACAGTGTCAGGGCAG7400.10972179593821774No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC7130.10571843311344492No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC6960.1031977972608102No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT6850.10156679759145831No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)