FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688992

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688992
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences674433
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC19860.2944695766666222No Hit
GATTTATAGTACAAGCAACAAATACTCCTGGACACCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGC16700.24761540434705895No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG14670.217516046812656No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC12340.18296850836183876No Hit
CACTAACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGCTTCCAGC12320.18267196296741114No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT12100.17940996362870737No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA12010.1780755093537831No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAAGACGCC11680.17318251034572743No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11580.17169978337358938No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10890.16146896726583665No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA10400.15420360510236006No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG10180.15094160576365628No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT10150.15049678767201485No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT10070.14931060609430438No Hit
CTATTACTGTTTACTCTACTTTGGTGCTGCTCAGGTGGGCGTCTTCGGCG9770.14486242517789016No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAAGACGCCCACCTGAGCA9590.14219351662804164No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG9570.141896971233614No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG9360.13878324459212404No Hit
GTGGTGACTCAGGAGCCCTCACTAACTGTGTCCCCAGGAGGGACAGTCAC8940.13255579130914413No Hit
GACTATTACTGTTTACTCTACTTTGGTGCTGCTCAGGTGGGCGTCTTCGG8600.12751451960387467No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG8450.12529042914566754No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT8440.12514215644845375No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA8350.12380770217352947No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC8350.12380770217352947No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTC7860.1165423400100529No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG7840.11624579461562527No Hit
CTATAAATCAGTGCCGTGGGTGCTTGTCCACGTTTCTGCTGGAACCAGTTTGGAAAGTAACCACTGGTGACTGCT7710.11431824955184579No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC7480.11090797751592821No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT7210.10690461469115538No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC7030.10423570614130685No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG6990.10364261535245163No Hit
GTAATAGTCAGCCTCGTCCTCAGGCTGCACACCCGACAGTGTCAGGGCAG6870.10186334298588591No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATTT300.00290004465.4641570
GGTACGT303.3042266E-452.44517568
TACGTAT400.00905450649.09811470
CTAACGC352.571061E-548.265143
GGTATCA8300.043.170281
CCTAACG405.6468758E-542.244532
GTATCAA20600.035.115891
GTGGTAT2700.031.2992121
TGGTATC2700.031.2922462
ATACACT652.67813E-531.1843954
TATCAAC23750.030.4516372
TCTAGGG450.005119311630.0316453
ATCAACG24550.029.5882033
TCAACGC24600.029.5258734
CAACGCA24950.029.1116835
AACGCAG25150.028.7458526
ACGCCAT606.1282533E-428.1525766
CACGACA500.007256513527.9616447
CGCAGAG26050.027.7527148
AGAGTAC26100.027.5701111
Produced by FastQC (version 0.11.5)