FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688993

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688993
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5622
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT186833.22660974742085No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCC2604.624688722874422No Hit
ATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT1723.059409462824618TruSeq Adapter, Index 8 (95% over 22bp)
TCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCG1132.009960868018499No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCGCGTATGCCGT921.6364283173247955No Hit
ACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCT871.5474919957310567RNA PCR Primer, Index 8 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTTTGCCGT851.511917467093561No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGGCGT801.4229811454998222No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGG781.3874066168623265No Hit
GAGACAGCTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTC450.8004268943436499No Hit
AGAGACAGCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCG440.7826396300249021No Hit
GTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACGCTCATGAA430.7648523657061544No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATTCCGT390.6937033084311632No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATATCGTATGCCGT320.5691924581999288No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATGTCGTATGCCGT290.5158306652436855No Hit
TATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCT260.46246887228744216RNA PCR Primer, Index 8 (95% over 21bp)
GAGACAGCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGT250.4446816079686944No Hit
GTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTG220.39131981501245106TruSeq Adapter, Index 8 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTC210.3735325506937033No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCCT170.30238349341871223No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCGCGTTTGCCGT170.30238349341871223No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCGCGTATGCCGG170.30238349341871223No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTTTGGCGT150.26680896478121663No Hit
AGAGACAGCTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCT150.26680896478121663No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATTGCGT140.24902170046246885No Hit
ATACACATCTCCGAGCCCACGAGACGCTCATGAATCGCGTATGCCGTCTT140.24902170046246885No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGGATGCCGT130.23123443614372108No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGGATGCCGG120.21344717182497333No Hit
GCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCG120.21344717182497333No Hit
AGAGACAGTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTC120.21344717182497333No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTGGTATGCCGT120.21344717182497333No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTTTGCCGG120.21344717182497333No Hit
GAGACAGTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCG120.21344717182497333No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCGCGTATGCC120.21344717182497333No Hit
CTTATACACATCTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAA110.19565990750622553No Hit
ATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTGTT100.17787264318747778No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATGTCGTATGCC100.17787264318747778No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTTTGCC90.16008537886872998No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTTTGCCG90.16008537886872998No Hit
GTGTATAAGAGACAGATACACATCTCCGAGCCCACGAGACGCTCATGAAT90.16008537886872998No Hit
GAGACAGCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA80.1422981145499822TruSeq Adapter, Index 8 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCGCGTATGGCGT80.1422981145499822No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCGCGTATTCCGT80.1422981145499822No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCC80.1422981145499822No Hit
CACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTG80.1422981145499822TruSeq Adapter, Index 8 (96% over 25bp)
ACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTGT80.1422981145499822Illumina PCR Primer Index 8 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATTCC70.12451085023123443No Hit
ATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTTTGCCGTCTT70.12451085023123443No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATGGCGTATGCCGT70.12451085023123443No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGGCGG70.12451085023123443No Hit
GTATAAGAGACAGATACACATCTCCGAGCCCACGAGACGCTCATGAATCT70.12451085023123443No Hit
GTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTT70.12451085023123443TruSeq Adapter, Index 8 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGGCGTCTT70.12451085023123443No Hit
GTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACGCTCATG70.12451085023123443No Hit
AGAGACAGCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCGCG70.12451085023123443No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATGTCGTTTGCCGT60.10672358591248667No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCGCGGATGCCGT60.10672358591248667No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGGC60.10672358591248667No Hit
CAGCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGC60.10672358591248667No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTAGTATGCCGT60.10672358591248667No Hit
ATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGGCTT60.10672358591248667No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAAGCTCGTATGCCGT60.10672358591248667No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTGGTATGGCGT60.10672358591248667No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATATCGTATTCCGT60.10672358591248667No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAATA150.001910236471.7383264
TGGGGGG550.071.2059470
GTAAAAA206.71093E-571.0740860
TTATGCT150.001983087771.0740752
TTGTAAA150.001983087771.0740758
TATGCTT150.001983087771.0740753
TGCTTTA150.001983087771.0740755
CTGCTTT150.001983087771.0740754
GGGGTGA150.001983087771.0740755
TGTAAAA252.2622771E-671.0740759
TGGCGTG150.002057991270.4220245
GGTGAAA352.2106178E-760.9206357
CTTGTGC352.2106178E-760.9206351
TTGTGCT352.2106178E-760.9206352
GGGTGAA352.2106178E-760.9206356
AGGGGGG1950.058.24280570
GTCTTGT452.0010702E-854.7726849
TCTTGTG405.564907E-753.30555750
GTGCGTG200.0062000553.30555754
TGTGCTT405.564907E-753.30555753
Produced by FastQC (version 0.11.5)