FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688995

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688995
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1445706
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTATATTGTTGGCAGTAATAAGTTGCAAAGTCTTCAGGCTGTAGGTTGCTGATGGTGAGAGTGAAATCTGTC170701.180737992371893No Hit
GTCTTCAGGCTGTAGGTTGCTGATGGTGAGAGTGAAATCTGTCCCAGACATACTGCCGCTGAACTTTGATGGGAC53320.3688163430185667No Hit
ATATAATGCTTACCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT46240.3198437303296798No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC44260.30614799966244866No Hit
ATAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGCATTAGAAATGATGTAGCCTGGTTTCAGCAGAAA40130.2775806422605979No Hit
GTCTCCATCCTCGCTGACTGCATCTATAGGAGACAGAGTCACCATCACTT39160.2708711176407928No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39030.2699719030010251No Hit
GTAATAAATAGCCGTGTCGTCACCTCTCAGGCTGTTCATTTCCAAATACA38040.26312403766740955No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT35930.24852909236041076No Hit
GTATCAACGCAGAGTACGGGTAAGCATTATATTGTTGGCAGTAATAAGTTGCAAAGTCTTCAGGCTGTAGGTTG33530.23192820670316097No Hit
GCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGT31620.21871666853426633No Hit
GGCTGTAGGTTGCTGATGGTGAGAGTGAAATCTGTCCCAGACATACTGCC31250.21615736532877364No Hit
GGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCT30280.20944784070896846No Hit
GAGTGAAATCTGTCCCAGACATACTGCCGCTGAACTTTGATGGGACCCCA28830.19941813895771338No Hit
CAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGCCAGATGTGACAT28830.19941813895771338No Hit
GCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCG28630.19803473181960923No Hit
AGTCAGGGCATTAGAAATGATGTAGCCTGGTTTCAGCAGAAACCAGGGAA28340.19602879146935823No Hit
GGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCC27960.19340031790696036No Hit
GTTTCCCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCG27550.19056433327384684No Hit
GTCCCAGACATACTGCCGCTGAACTTTGATGGGACCCCACTTTGCAAATT27420.18966511863407914No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27110.1875208375700177No Hit
GACCCAGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGG26890.1859990897181031No Hit
GAGTACGGGTAAGCATTATATTGTTGGCAGTAATAAGTTGCAAAGTCTTC26630.18420066043856773No Hit
GTAATAAGTTGCAAAGTCTTCAGGCTGTAGGTTGCTGATGGTGAGAGTGAAATCTGTCCCAGACATACTGCCG26560.18371646794023128No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24700.17085078155586267No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24490.16939820406085332No Hit
GTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCC24440.16905235227632728No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23300.16116693158913362No Hit
CTTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGCTACATCATTTCTAA22830.15791592481458885No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT22590.15625583624886388No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22200.15355819232956078No Hit
GACTTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGCTACATCATTTCT22090.1527973184036035No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21800.1507913780533525No Hit
ATTTATTACTGTGCGAGCGTGGCTGACTACTGGGGCCAGGGAACCCTGGT21580.1492696302014379No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA21370.14781705270642853No Hit
ATATTGTTGGCAGTAATAAGTTGCAAAGTCTTCAGGCTGTAGGTTGCTGATGGTGAGAGTGAAATCTGTCCCAGA21280.14719451949428167No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21110.14601862342689315No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20940.14484272735950462No Hit
GGACCCCACTTTGCAAATTGGATGCACCAAAAAGCAGGGACTTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGG19970.13813320273969948No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT19830.13716481774302658No Hit
GCCCCTAAGTCCCTGCTTTTTGGTGCATCCAATTTGCAAAGTGGGGTCCCATCAAAGTTCAGCGGCAGTATGT19640.13585058096182764No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19630.13578141060492244No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19590.1355047291773016No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19500.13488219596515474No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG19360.13391381096848184No Hit
GTCTCCTATAGATGCAGTCAGCGAGGATGGAGACTGGGTCATCTGGATGTCACATCTGGCACCTGGGAAACAGAG19130.13232289275966208No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT19000.13142367811989436No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG18420.12741179741939232No Hit
GTCTGGGGGAGGCGTGGTCGAGCCTGGGAGGTCCCTGAGACTCTCCTGTG18070.12499083492771006No Hit
GGGCATTAGAAATGATGTAGCCTGGTTTCAGCAGAAACCAGGGAAAGCCCCTAAGTCCCTGCTTTTTGGTGCATC18040.12478332385699445No Hit
CATCTATAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGCATT17730.122639042792933No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAACCTACAGCCTGAAGACT17720.12256987243602781No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17660.12215485029459655No Hit
CCAAAAAGCAGGGACTTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGC17460.1207714431564924No Hit
GATGTAGCCTGGTTTCAGCAGAAACCAGGGAAAGCCCCTAAGTCCCTGCT17380.12021808030125074No Hit
CTATAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGCATTAGA17000.11758960673885284No Hit
GGTATCAACGCAGAGTACGGGTAAGCATTATATTGTTGGCAGTAATAAGTTGCAAAGTCTTCAGGCTGTAGGTTG16900.11689790316980078No Hit
ATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGTCCACCT16720.11565283674550705No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC16690.1154453256747914No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG16610.11489196281954976No Hit
ACTTTGATGGGACCCCACTTTGCAAATTGGATGCACCAAAAAGCAGGGACTTAGGGGCTTTCCCTGGTTTCTG16600.11482279246264454No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC16410.11350855568144559No Hit
CACCATCACTTGTCGGGCGAGTCAGGGCATTAGAAATGATGTAGCCTGGTTTCAGCAGAAACCAGGGAAAGCCC15990.11060340069142689No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15820.10942750462403836No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15650.10825160855664982No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15600.10790575677212379No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCACTGCATGGCATACGTAG15380.10638400892020923No Hit
CTCCTGTGCAGCCTCTGGATTCAGCGTTACTACGTATGCCATGCAGTGGGTCCGCCAGGCTCCAGGCAAGGGG15310.10589981642187278No Hit
GTATCAACGCAGAGTACGGGAATCAGACCCAGTCAGGACACAGCATGGAC15280.10569230535115715No Hit
GTGGGGTCCCATCAAAGTTCAGCGGCAGTATGTCTGGGACAGATTTCACT15210.1052081128528207No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC15110.10451640928376862No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15080.104308898213053No Hit
GCACTGGAAGTCGCCGGTGTTTCCATTCGGTGATCATCACTGAACACAGA15030.10396304642852697No Hit
CCTATAGATGCAGTCAGCGAGGATGGAGACTGGGTCATCTGGATGTCACA15020.10389387607162176No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT14930.10327134285947488No Hit
GAGTCACCATCACTTGTCGGGCGAGTCAGGGCATTAGAAATGATGTAGCCTGGTTTCAGCAGAAACCAGGGAAAG14930.10327134285947488No Hit
GCCCTGACTCGCCCGACAAGTGATGGTGACT14720.10181876536446553No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14600.10098872108160303No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14460.10002033608493012No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)