FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688996

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688996
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1445706
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTATATTGTTGGCAGTAATAAGTTGCAAAGTCTTCAGGCTGTAGGTTGCTGATGGTGAGAGTGAAATCTGTC161631.1180004786588698No Hit
GTCTTCAGGCTGTAGGTTGCTGATGGTGAGAGTGAAATCTGTCCCAGACATACTGCCGCTGAACTTTGATGGGAC58090.4018106032623507No Hit
ATATAATGCTTACCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT43660.3019977782481362No Hit
GTCTCCATCCTCGCTGACTGCATCTATAGGAGACAGAGTCACCATCACTT39590.27384544298771674No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC39490.27315373941866467No Hit
GGCTGTAGGTTGCTGATGGTGAGAGTGAAATCTGTCCCAGACATACTGCC37860.2618789712431158No Hit
GTATCAACGCAGAGTACGGGTAAGCATTATATTGTTGGCAGTAATAAGTTGCAAAGTCTTCAGGCTGTAGGTTG36260.2508117141382826No Hit
ATAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGCATTAGAAATGATGTAGCCTGGTTTCAGCAGAAA35760.24735319629302222No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT35590.2461773002256337No Hit
GTCCCAGACATACTGCCGCTGAACTTTGATGGGACCCCACTTTGCAAATT35160.2432029748787098No Hit
GTAATAAATAGCCGTGTCGTCACCTCTCAGGCTGTTCATTTCCAAATACA34160.23628593918818905No Hit
GAGTGAAATCTGTCCCAGACATACTGCCGCTGAACTTTGATGGGACCCCA33240.22992226635290994No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31550.21823247603592982No Hit
GGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCT28680.1983805836041353No Hit
GCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCG28350.19609796182626346No Hit
GCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGT28330.19595962111245302No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27230.18835088185288018No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27210.18821254113906977No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26760.18509987507833542No Hit
GAGTACGGGTAAGCATTATATTGTTGGCAGTAATAAGTTGCAAAGTCTTC26280.18177969794688548No Hit
GTTTCCCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCG26130.18074214259330734No Hit
GGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCC25660.17749113581876258No Hit
GTAATAAGTTGCAAAGTCTTCAGGCTGTAGGTTGCTGATGGTGAGAGTGAAATCTGTCCCAGACATACTGCCG25140.1738942772596918No Hit
CAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGCCAGATGTGACAT24970.17271838119230326No Hit
AGTCAGGGCATTAGAAATGATGTAGCCTGGTTTCAGCAGAAACCAGGGAA24870.17202667762325122No Hit
GACCCAGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGG24370.16856815977799083No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23290.1610977612322284No Hit
GACTTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGCTACATCATTTCT23270.160959420518418No Hit
GTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCC22680.15687836946101075No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21430.1482320748478598No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21130.1461569641407036No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC20720.14332097950759007No Hit
ATATTGTTGGCAGTAATAAGTTGCAAAGTCTTCAGGCTGTAGGTTGCTGATGGTGAGAGTGAAATCTGTCCCAGA20570.14228342415401196No Hit
CTTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGCTACATCATTTCTAA20550.14214508344020152No Hit
ATTTATTACTGTGCGAGCGTGGCTGACTACTGGGGCCAGGGAACCCTGGT20020.13847905452422554No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA19890.13757983988445785No Hit
GTGGGGTCCCATCAAAGTTCAGCGGCAGTATGTCTGGGACAGATTTCACT19560.135297218106586No Hit
GTCTCCTATAGATGCAGTCAGCGAGGATGGAGACTGGGTCATCTGGATGTCACATCTGGCACCTGGGAAACAGAG19350.13384464061157664No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19350.13384464061157664No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19100.13211538168894643No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19000.13142367811989436No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT18960.13114699669227353No Hit
GGACCCCACTTTGCAAATTGGATGCACCAAAAAGCAGGGACTTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGG18940.13100865597846312No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG18890.13066280419393708No Hit
GTCTGGGGGAGGCGTGGTCGAGCCTGGGAGGTCCCTGAGACTCTCCTGTG18830.13024778205250584No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18290.12651258277962463No Hit
GCCCCTAAGTCCCTGCTTTTTGGTGCATCCAATTTGCAAAGTGGGGTCCCATCAAAGTTCAGCGGCAGTATGT18230.12609756063819338No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAACCTACAGCCTGAAGACT18060.12492166457080485No Hit
GGTATCAACGCAGAGTACGGGTAAGCATTATATTGTTGGCAGTAATAAGTTGCAAAGTCTTCAGGCTGTAGGTTG17860.12353825743270071No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17580.1216014874393549No Hit
GGGCATTAGAAATGATGTAGCCTGGTTTCAGCAGAAACCAGGGAAAGCCCCTAAGTCCCTGCTTTTTGGTGCATC17320.11980305815981948No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT17170.11876550280624139No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17020.11772794745266327No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16750.11586034781622266No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16200.11205597818643624No Hit
ACTTTGATGGGACCCCACTTTGCAAATTGGATGCACCAAAAAGCAGGGACTTAGGGGCTTTCCCTGGTTTCTG16090.11129510426047895No Hit
CTCCTGTGCAGCCTCTGGATTCAGCGTTACTACGTATGCCATGCAGTGGGTCCGCCAGGCTCCAGGCAAGGGG15950.11032671926380606No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15740.1088741417687967No Hit
GTATCAACGCAGAGTACGGGAATCAGACCCAGTCAGGACACAGCATGGAC15570.10769824570140818No Hit
CTATAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGCATTAGA15460.10693737177545087No Hit
GTAGGTTGCTGATGGTGAGAGTGAAATCTGTCCCAGACATACTGCCGCTG15410.10659151999092484No Hit
CCAAAAAGCAGGGACTTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGC15350.10617649784949361No Hit
GCACTGGAAGTCGCCGGTGTTTCCATTCGGTGATCATCACTGAACACAGA15260.10555396463734674No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15250.10548479428044152No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAA15240.10541562392353632No Hit
CATCTATAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGCATT15190.10506977213901028No Hit
CTGCTGCTCTGTTTCCCAGGTGCCAGATGTGACATCCAGATGACCCAGTC15100.10444723892686342No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14750.10202627643518115No Hit
GGGCTGGAGTGGGTGTCTTTGGTATCACATGATGGGAATACTAAAGACTA14640.10126540250922386No Hit
CACCATCACTTGTCGGGCGAGTCAGGGCATTAGAAATGATGTAGCCTGGTTTCAGCAGAAACCAGGGAAAGCCC14610.10105789143850824No Hit
GAGTCACCATCACTTGTCGGGCGAGTCAGGGCATTAGAAATGATGTAGCCTGGTTTCAGCAGAAACCAGGGAAAG14570.10078121001088741No Hit
GATGTAGCCTGGTTTCAGCAGAAACCAGGGAAAGCCCCTAAGTCCCTGCT14560.1007120396539822No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCACTGCATGGCATACGTAG14510.10036618786945617No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT5200.048.121321
GTATCAA49700.038.2374651
TCTGGAG13800.035.32922470
TCCTATA4950.034.1364634
CTAACGC502.1008404E-433.7974363
CCGCTAT1651.0957774E-732.83119670
AATGGTA4750.032.78799470
TATCAAC57850.032.427872
ATCAACG59250.031.6013183
CTCCTAT5400.031.2939243
TCAACGC59950.031.1737924
CAACGCA60700.030.8464245
TGTTAGA12750.030.80338770
AACGCAG61250.030.6787326
GCTTGTA1001.7631464E-830.4334851
CGCAGAG62950.029.688158
GTCTCCT6000.029.3063181
GGTATCA31150.029.2013841
GCAGAGT64650.029.1175889
AGAGTAC64800.029.04817811
Produced by FastQC (version 0.11.5)