FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688997

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688997
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences687890
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACACCCGTGTCGTCAGTTCTCAGGCCGCTCATTTGAAGATACA31490.45777667941095235No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC21040.3058628559798805No Hit
GTCTATTACTGTGCGAGAACGGTGTGTAGTGGTGGAAGCTGCTACCGAAG20200.2936516012734594No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16040.23317681606070736No Hit
CCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACG15780.2293971419849104No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14930.21704051519865095No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13850.20134033057610956No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG13430.19523470322289901No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC12630.1836049368358313No Hit
GTATTGGTCGTCTATGAAGATAGCGCCCGGCCCTCAGGGATCCCTGAGCG12550.18244196019712455No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11730.17052144965038013No Hit
ATACAGGGCCCTGGCCTGTCTTCTGCTGGTACCAGTGCACACTTTTAGTT11560.16805012429312827No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT11080.16107226446088765No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC11040.16049077614153426No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10980.1596185436625042No Hit
GCCCTGTATTGGTCGTCTATGAAGATAGCGCCCGGCCCTCAGGGATCCCT10830.157437962464929No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC10780.15671110206573724No Hit
CAGTAATAGACACCCGTGTCGTCAGTTCTCAGGCCGCTCATTTGAAGATA10740.15612961374638387No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA10740.15612961374638387No Hit
GTCCATCAGCAGGGTCGAGGCCGGGGATGAGGCCGACTATTACTGTCAGG10570.15365828838913198No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10550.15336754422945528No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCGCCGCCGAACACCCAATGTTGATGATCATTAATAAGATCC10540.15322217214961695No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10330.15016935847301166No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10300.14973324223349663No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA10250.1490063818343049No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC10240.14886100975446656No Hit
CTTATTTACAGTGCTGGAAGCACATTCTACGCAGACTCCGTGAGGGGCCG10060.1462443123173763No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT9980.14508133567866954No Hit
GCATTACCTGTGGGGGAGACAACATTGGAACTAAAAGTGTGCACTGGTACCAGCAGAAGACAGGCCAGGGCC9840.1430461265609327No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC9720.14130166160287255No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC9700.14101091744319585No Hit
GAGTTTGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGGTGTCCAGTGTCAGGTCCACTTGGTGGAAT9540.1386849641657823No Hit
GAACACAGAGGACTCGCCATGGAGTTTGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGGTGTCCAGTGT9470.1376673596069139No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9270.13475991801014697No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT9270.13475991801014697No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT9240.13432380177063194No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCGC9220.13403305761095524No Hit
CGGCCACCCTGTCCATCAGCAGGGTCGAGGCCGGGGATGAGGCCGACTAT9080.13199784849321838No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG8980.13054412769483492No Hit
CCCCAGGACAGACGGCCAGCATTACCTGTGGGGGAGACAACATTGGAACT8850.12865429065693643No Hit
CTATTACTGTGCGAGAACGGTGTGTAGTGGTGGAAGCTGCTACCGAAGACTATATCACGGTATGGACTTCTGGGG8850.12865429065693643No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT8710.1266190815391996No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT8700.12647370945936123No Hit
GGGCTGGAGTGGGTCTCACTTATTTACAGTGCTGGAAGCACATTCTACGC8670.1260375932198462No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG8660.12589222114000784No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC8650.1257468490601695No Hit
GGCCAGGGCCCTGTATTGGTCGTCTATGAAGATAGCGCCCGGCCCTCAGG8630.1254561049004928No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG8600.12501998866097777No Hit
CTATATCACGGTATGGACTTCTGGGGCCAAGGGACCACGGTCACCGTCTC8590.12487461658113944No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG8540.12414775618194769No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT8500.12356626786259432No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT8370.12167643082469581No Hit
GCACTGAACACAGAGGACTCGCCATGGAGTTTGGGCTGAGCTGGGTTTTC8350.12138568666501912No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG8240.11978659378679732No Hit
GTGTGTAGTGGTGGAAGCTGCTACCGAAGACTATATCACGGTATGGACTT8210.11935047754728226No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC8210.11935047754728226No Hit
GGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGGCGCTATCTTCATAGA8170.11876898922792889No Hit
GATCTGCACTGAACACAGAGGACTCGCCATGGAGTTTGGGCTGAGCTGGG8010.11644303595051535No Hit
GTCTCTGGATTCACCGTCAGTTACAGTTACGTGAGTTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGT7990.11615229179083866No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC7790.11324485019407171No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT7760.11280873395455668No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACT7750.11266336187471834No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA7740.11251798979488No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG7730.11237261771504166No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC7730.11237261771504166No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCGTGGTCCCTTGG7570.11004666443762812No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC7490.10888368779892134No Hit
GCCCAGCACTGGGATTCCGAGGTGTTTCCAATCAGTGATCTGCACTGAAC7480.10873831571908299No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC7450.10830219947956796No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG7370.10713922284086119No Hit
GCAGAAGACAGGCCAGGGCCCTGTATTGGTCGTCTATGAAGATAGCGCCCGGCCCTCAGGGATCCCTGAGCGATT7290.10597624620215441No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT7240.10524938580296268No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG7050.10248731628603411No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT7030.10219657212635741No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG7010.10190582796668073No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGG6970.10132433964732733No Hit
GTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGT6920.10059747924813561No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAACTCACGTAACTGTAAC6910.10045210716829725No Hit
GTGTAGTGGTGGAAGCTGCTACCGAAGACTATATCACGGTATGGACTTCTGGGGCCAAGGGACCACGGTCACCGT6890.10016136300862055No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCAT300.002913676465.3871770
CTTGTAT401.110103E-465.3871670
TCTCGTA850.052.69038438
TGAACGG200.006858417852.67788353
CGTATGC1150.048.0663741
AGGGGGG1650.047.554370
TCGTATG1100.047.07241440
TCTGGAG9700.043.8161470
ATATAGC404.8272515E-543.38247345
TATACAC1500.043.2545363
ATGCCGT1350.041.09050444
GGTATCA5600.040.8619651
GACAGGT1350.040.477971
GTATCAA14000.039.276281
GTCGTAC350.001416025239.0323261
TCGTACC350.001417037839.0266532
ATCTCGT1150.038.91081637
TATAGCC451.0457682E-438.05047231
ACGAGAC1450.037.67542622
CGTCTTC1400.037.32010348
Produced by FastQC (version 0.11.5)