FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688999

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688999
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1125298
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGAGAGTTCACCGTACACTTTTGGCCAGGGGA44500.3954508050312006No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32120.2854355024180262No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31090.27628237142516915No Hit
GTAATAAACAGCCGTGTCGTCGACTCTCAGGCTGTTCATTTGCAGATACA27740.2465124793610226No Hit
GTATGAGAGTTCACCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT27730.2464236140115774No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA24070.2138988961146292No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22880.20332391953064877No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT20760.18448446544826347No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA20470.18190737031435228No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20120.17879708308376982No Hit
GTTTATTACTGTGCGAGACATGCAGCAGTGCCTGGTTTTGACTCCTGGGGCCAGGGAACCCTGGTCACCGTCTC20040.1780861602882081No Hit
GTGTATTACTGTCAGCAGTATGAGAGTTCACCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA19760.17559793050374212No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC19360.17204331652593358No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18780.1668891262581112No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18520.16457862717253563No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18110.16093514784528187No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17720.15746939921691855No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17610.1564918803730212No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17570.15613641897524033No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA17050.15151542080408922No Hit
CTCTCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC17050.15151542080408922No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT16520.1468055572834929No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16210.14405073145069128No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT15910.14138477096733487No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15800.14040725212343752No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14650.13018773693723795No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14550.12929908344278582No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG14020.1245892199221895No Hit
GTACCTTTACTGTGCACTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAG14010.12450035457274428No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13950.123967162476073No Hit
CTCTTGCAGGGCCAGTCGGACTATTGGCAGCACCTACGTAGCCTGGTTCC13830.12290077828273044No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA13820.12281191293328524No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT13650.12130120199271659No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT13490.11987935640159317No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC13400.11907956825658625No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12940.11499176218210644No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12910.11472516613377079No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12790.11365878194042822No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12670.11259239774708567No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGGT12530.11134828285485267No Hit
CTCCTGTGCAGCCTCTGGATTCGCCTTCAGTACCTTTACTGTGCACTGGGTCCGCCAGGCTCCAGGGAAGGGG12380.11001530261317448No Hit
GTCTTTGTCTCCAGGGGACAGAGCCACCCTCTCTTGCAGGGCCAGTCGGA12340.1096598412153936No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG12000.10663841933425634No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11990.10654955398481114No Hit
GGCCAAAAGTGTACGGTGAACTCTCATACTGCTGACAGTAATACACTGCA11590.1029949400070026No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG11440.10166195976532438No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGAGAGTTCA11330.10068444092142703No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTCGT503.9078566E-450.6925970
TCGTATG2900.042.86247340
GGTATCA13850.042.5672571
CGTATGC3000.041.50475741
ACATGCG2950.040.4679927
GCGGCAT808.261164E-539.6035870
ACGAGAC3200.039.40491522
ATCTCGT2650.038.91840437
ATGCGCA3000.038.70133229
GTATCAA34550.037.1854131
CCCACGA3400.037.07873519
ATGCCGT3400.036.77280844
TCTGGAG9550.036.4933570
ATCTCCG3500.036.01614410
GCCGTCT3400.035.84552446
CATGCGC3250.035.7020428
CTTATAC3500.035.052081
CCGTCTT3500.034.87363447
CGAGCCC3650.034.5360315
CACGAGA3700.034.07992621
Produced by FastQC (version 0.11.5)