FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689001

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689001
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1689873
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAACAATATCATGTTTGGCCTCCGATGACCTTCGGCGGGGGGACCAAGGTGGAGATCAAGCGAA106680.6312900436896738No Hit
GTACTATTGTGTGAGATGTCGCGGATTCAGTTATCATTTTGGGGGATTCTTCGACAAGTGGGGCCAGGGAAAC62960.3725723767407373No Hit
CAGTAATACAGTCCAAAATCTTCAGGCTGCAGGCCGTTGATGGTGAGAGTGAAGTCTGTCTGAGACCCACTGCCA59080.3496120714396881No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT58710.34742255778984577No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC53930.3191364084756665No Hit
CAATAGTACAGGGCCGTGTCCTCGGCTTTCAGGTTATTCATTTGTAAGGA52340.3097274173858035No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC43260.2559955688977811No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC40970.2424442546865948No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC36490.21593338671012557No Hit
GCCCTGTACTATTGTGTGAGATGTCGCGGATTCAGTTATCATTTTGGGGG34590.20468993823796228No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34210.20244124854352957No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT33240.1967011722182673No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT33230.19664199617367698No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG32530.19249967305235363No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG32460.19208544074022132No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG29760.17610790870083134No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29670.1755753242995184No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA29650.1754569722103377No Hit
GTCTCAGACAGACTTCACTCTCACCATCAACGGCCTGCAGCCTGAAGATT29530.1747468596752537No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29430.17415509922935038No Hit
GTACCAACAGAGGCCTGGCCAGGCGCCCAGGCTCCTCATTTATGGTGCAATTACAAGGGAGACTGGTGTCCCAGC28820.17054536050934005No Hit
CTCCTGTGCAGACTCGGGATTCGCCTTGACTAATTATTGGGTGACCTGGGTTCGCCAGGCTCCAGGGAAGGGA28780.17030865633097872No Hit
GTGTTAGCACCAACTTAGCCTGGTACCAACAGAGGCCTGGCCAGGCGCCCAGGCTCCTCATTTATGGTGCAATTA28750.17013112819720771No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA28680.16971689588507538No Hit
GGACTGGAGTGGGTGGCCAGTGTGAAGTATGATGGAAGTGAACATAAGTA28500.16865172708244938No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCACCAACTTAGCCTGGTACCAACAGAGGCCTGGCCAGGCGCCCAGGCT28080.1661663332096554No Hit
GTCTCCAGACACCCTGTCTGTGTCTCCAGGGGCAAGAGTCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAC27290.16149142568701907No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27270.16137307359783842No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26530.15699404629815375No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26000.1538577159348661No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATCTCCACCTTGGTCC25280.14959704072436214No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC25070.14835434378796514No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC25060.14829516774337478No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT24940.1475850552082908No Hit
CTCCAGTCCCTTCCCTGGAGCCTGGCGAACCCAGGTCACCCAATAATTAG24320.14391614044369014No Hit
GTCTGAGACCCACTGCCACTGAATCGGGCTGGGACACCAGTCTCCCTTGT23840.14107569030335415No Hit
CCCTGTCTGTGTCTCCAGGGGCAAGAGTCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCACCAACTTAGC23690.14018804963449916No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG23590.13959628918859582No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23420.13859029643056014No Hit
GTACAGGGCCGTGTCCTCGGCTTTCAGGTTATTCATTTGTAAGGAGACTT23210.13734759949416317No Hit
GTCAGAGTGTTAGCACCAACTTAGCCTGGTACCAACAGAGGCCTGGCCAGGCGCCCAGGCTCCTCATTTATGGTG23020.13622325464694685No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG22900.13551314211186283No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAACTAGTGGGCAAGTAGTT22630.13391538890792384No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA22340.1321992836148042No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT22300.13196257943644288No Hit
GACTTCACTCTCACCATCAACGGCCTGCAGCCTGAAGATTTTGGACTGTATTACTGTCAACAATATCATGTTTGG22290.13190340339185253No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA22290.13190340339185253No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT22120.13089741063381685No Hit
GGCCTGGCCAGGCGCCCAGGCTCCTCATTTATGGTGCAATTACAAGGGAG21860.12935883347446822No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG21850.12929965742987787No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT21740.1286487209393842No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC21220.1255715666206869No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC21090.12480227804101256No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT20830.12326370088166388No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20410.12077830700886989No Hit
GTGCAGCCACAGTTCGCTTGATCTCCACCTTGGTCCCCCCGCCGAAGGTC20180.11941725798329224No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19840.1174052724672209No Hit
GAGTCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCACCAACTTAGCCTGGTACCAACAGAGGCCTGGCCAGG19800.11716856828885958No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC19750.11687268806590792No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG19680.11645845575377557No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG19480.11527493486196891No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19400.11480152650524625No Hit
GAGTTGGGGCTGAGCTGGCTTTTCCTTGTTGCTATTTTAGAAGGTGTCCA19380.1146831744160656No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGAGACCAGGTTTCCCTGG19060.11278954098917493No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGC18970.11225695658786193No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC18830.11142849196359728No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18790.11119178778523595No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT18370.10870639391244194No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC18350.10858804182326129No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18350.10858804182326129No Hit
ACAGAGGCCTGGCCAGGCGCCCAGGCTCCTCATTTATGGTGCAATTACAAGGGAGACTGGTGTCCCAGCCCGATT18200.10770040115440627No Hit
GAGTGAAGTCTGTCTGAGACCCACTGCCACTGAATCGGGCTGGGACACCA18180.10758204906522562No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC17800.10533335937079294No Hit
CTCCAGACACCCTGTCTGTGTCTCCAGGGGCAAGAGTCACCCTCTCCTGC17740.10497830310325096No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC17700.10474159892488963No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC17690.10468242288029929No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG17690.10468242288029929No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT17410.10302549363176995No Hit
AACTTAGCCTGGTACCAACAGAGGCCTGGCCAGGCGCCCAGGCTCCTCATTTATGGTGCAATTACAAGGGAGA17200.10178279669537296No Hit
GTGTGAAGTATGATGGAAGTGAACATAAGTATTTGGATTCTGTGGAGGGC17170.10160526856160196No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17160.10154609251701162No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)