FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689002

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689002
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1689873
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAACAATATCATGTTTGGCCTCCGATGACCTTCGGCGGGGGGACCAAGGTGGAGATCAAGCGAA100830.5966720576043288No Hit
GTACTATTGTGTGAGATGTCGCGGATTCAGTTATCATTTTGGGGGATTCTTCGACAAGTGGGGCCAGGGAAAC62530.370027806823353No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT58260.3447596357832807No Hit
CAGTAATACAGTCCAAAATCTTCAGGCTGCAGGCCGTTGATGGTGAGAGTGAAGTCTGTCTGAGACCCACTGCCA54990.32540906920224183No Hit
CAATAGTACAGGGCCGTGTCCTCGGCTTTCAGGTTATTCATTTGTAAGGA44740.26475362349715037No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC44360.2625049338027177No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC42710.2527408864453128No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC39090.23131915830361216No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT34470.20397982570287826No Hit
GCCCTGTACTATTGTGTGAGATGTCGCGGATTCAGTTATCATTTTGGGGG34120.20190866414221662No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33600.1988315098235193No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC33220.19658282012908662No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT32200.19054686358087264No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31240.18486596330020066No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30920.18297232987331No Hit
CTCCTGTGCAGACTCGGGATTCGCCTTGACTAATTATTGGGTGACCTGGGTTCGCCAGGCTCCAGGGAAGGGA29320.1735041627388567No Hit
GTCTCAGACAGACTTCACTCTCACCATCAACGGCCTGCAGCCTGAAGATT29060.17196558557950806No Hit
GGACTGGAGTGGGTGGCCAGTGTGAAGTATGATGGAAGTGAACATAAGTA28260.1672315020122814No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28110.1663438613434264No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT28100.16628468529883605No Hit
GTGTTAGCACCAACTTAGCCTGGTACCAACAGAGGCCTGGCCAGGCGCCCAGGCTCCTCATTTATGGTGCAATTA27170.16078131315193508No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA26380.15610640562929876No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCACCAACTTAGCCTGGTACCAACAGAGGCCTGGCCAGGCGCCCAGGCT26280.15551464518339544No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC26040.15409442011322744No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG25930.15344348362273377No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25820.1527925471322401No Hit
GTACCAACAGAGGCCTGGCCAGGCGCCCAGGCTCCTCATTTATGGTGCAATTACAAGGGAGACTGGTGTCCCAGC25580.15137232206207213No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA25530.15107644183912045No Hit
GTCTCCAGACACCCTGTCTGTGTCTCCAGGGGCAAGAGTCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAC25350.15001127303649445No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25010.14799928752042313No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA24920.14746670311911014No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG24770.14657906245025515No Hit
GTCTGAGACCCACTGCCACTGAATCGGGCTGGGACACCAGTCTCCCTTGT24390.14433037275582247No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG24110.14267344350729316No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT23340.13811688807383748No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATCTCCACCTTGGTCC23240.13752512762793417No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22690.13427044517546585No Hit
CCCTGTCTGTGTCTCCAGGGGCAAGAGTCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCACCAACTTAGC22400.1325543398823462No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA22370.13237681174857518No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAACTAGTGGGCAAGTAGTT22110.13083823458922653No Hit
GACTTCACTCTCACCATCAACGGCCTGCAGCCTGAAGATTTTGGACTGTATTACTGTCAACAATATCATGTTTGG22060.13054235436627487No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21990.13012812205414254No Hit
GTCAGAGTGTTAGCACCAACTTAGCCTGGTACCAACAGAGGCCTGGCCAGGCGCCCAGGCTCCTCATTTATGGTG21740.1286487209393842No Hit
CTCCAGTCCCTTCCCTGGAGCCTGGCGAACCCAGGTCACCCAATAATTAG21290.12598579893281922No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC21120.12497980617478355No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC21100.1248614540856029No Hit
GGCCTGGCCAGGCGCCCAGGCTCCTCATTTATGGTGCAATTACAAGGGAG21030.12444722177347055No Hit
GTACAGGGCCGTGTCCTCGGCTTTCAGGTTATTCATTTGTAAGGAGACTT20800.12308617274789288No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC20650.1221985320790379No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT20330.12030489865214723No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT20260.11989066634001491No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20050.11864796940361791No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC20050.11864796940361791No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT20010.11841126522525658No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC19710.11663598388754659No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19590.11592587135246259No Hit
GTGCAGCCACAGTTCGCTTGATCTCCACCTTGGTCCCCCCGCCGAAGGTC19520.11551163904033024No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG19400.11480152650524625No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG19200.11361800561343958No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGAGACCAGGTTTCCCTGG19190.11355882956884927No Hit
GAGTTGGGGCTGAGCTGGCTTTTCCTTGTTGCTATTTTAGAAGGTGTCCA19160.11338130143507825No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18700.11065920338392293No Hit
GTGTGAAGTATGATGGAAGTGAACATAAGTATTTGGATTCTGTGGAGGGC18630.11024497107179061No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT18580.10994909084883894No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG18510.10953485853670661No Hit
GAGTGAAGTCTGTCTGAGACCCACTGCCACTGAATCGGGCTGGGACACCA18340.10852886577867095No Hit
GTCTGTGTCTCCAGGGGCAAGAGTCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCACCAACTTAGCCTGGTA18330.10846968973408061No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17870.10574759168292529No Hit
GAGTCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCACCAACTTAGCCTGGTACCAACAGAGGCCTGGCCAGG17820.10545171145997362No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17690.10468242288029929No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC17430.10314384572095063No Hit
CTGCTACTCTGGCTCCCAGAAACTAGTGGGCAAGTAGTTCTGACGCAGTC17140.10142774042783097No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG17060.10095433207110831No Hit
ACAGAGGCCTGGCCAGGCGCCCAGGCTCCTCATTTATGGTGCAATTACAAGGGAGACTGGTGTCCCAGCCCGATT16900.10000751535766297No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15600.045.1615561
GTGGTAT4000.042.7500151
TGGTATC4500.037.9831432
GTATCAA33850.034.75571
GACAGGT3750.032.8320161
ATGGCAA3350.032.17611370
GTGCTAG1950.031.5692421
ATACGTA1900.030.58009311
TATCAAC38200.030.4262892
TCAACGC39200.029.8218734
CAACGCA39700.029.3601825
TCCGATG29250.029.22288131
AACGCAG40050.029.10366
GAATACG2000.029.0536679
CATAGCG1502.6975613E-928.73762769
CTCCGAT30000.028.58719830
GGTACTA605.725395E-428.484833
CGAGTTC755.229272E-528.27508260
CTAGAGA2300.028.2371334
CGCTTCG18700.028.10032870
Produced by FastQC (version 0.11.5)