FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004689005

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689005
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences690694
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT34760.5032619365449823No Hit
GTATTACTGTCAGCAGTATGGCAGTTCACCTCCCACTTTCGGCGGAGGGA20920.3028837661829985No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT16350.2367184310273435No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15920.2304928086822819No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11950.17301438842671285No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTACTAATGATGAGAGC11870.1718561331067014No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA10820.15665403203155087No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10270.14869102670647202No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10030.14521626074643765No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT10010.14492669691643478No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9810.1420310586164061No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9750.14116236712639751No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9670.14000411180638606No Hit
GTGTATTACTGTCAGCAGTATGGCAGTTCACCTCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA9400.13609500010134734No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9340.13522630861133875No Hit
CATCTATGCTGCATCCAGCGGGTCCGCTGGCATCCCAGACAGGTTCACTGGCAGTGGGTCTGGGACAGACTTCG9320.13493674478133585No Hit
GTCTGGGACAGACTTCGCTCTCATCATTAGTAGACTGGAGCCTGAAGATT8870.12842155860627139No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT8640.1250915745612384No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCC8480.12277506392121547No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT8240.11930029796118108No Hit
GTATGGCAGTTCACCTCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT8190.1185763883861739No Hit
ATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGTCCACCT8040.1164046596611524No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAACAACTACTTAGCCTGGTACC7940.11495684051113808No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG7500.10858643625107502No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG7310.10583557986604777No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGGGAGGTGAA7280.10540123412104346No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAA7020.1016369043310062No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC6980.10105777667100047No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)