FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689006

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689006
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences690694
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG119961.7368038523571945No Hit
GTATTACTGTCAGCAGTATGGCAGTTCACCTCCCACTTTCGGCGGAGGGA21680.3138871917231075No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT14880.21543548952213282No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13030.18865083524686765No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11560.16736789374165695No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTACTAATGATGAGAGC10990.15911532458657524No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10830.15679881394655232No Hit
GTGTATTACTGTCAGCAGTATGGCAGTTCACCTCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA10480.15173144692150214No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA10320.1494149362814792No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9310.13479196286633444No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9090.1316067607363029No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9060.1311724149912986No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT8550.12378853732622551No Hit
GTCTGGGACAGACTTCGCTCTCATCATTAGTAGACTGGAGCCTGAAGATT8550.12378853732622551No Hit
CATCTATGCTGCATCCAGCGGGTCCGCTGGCATCCCAGACAGGTTCACTGGCAGTGGGTCTGGGACAGACTTCG8180.11843160647117246No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8120.11756291498116386No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG7800.112929893701118No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7790.11278511178611657No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG7770.1124955479561137No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAACAACTACTTAGCCTGGTACC7740.11206120221110939No Hit
GTATGGCAGTTCACCTCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT7720.11177163838110653No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT7260.1051116702910406No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT7210.10438776071603342No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG7180.10395341497102914No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG7170.1038086330560277No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT7050.1020712500760105No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTACTAATGATGAGAGCGA7000.10134734050100333No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAACG456.1299943E-1059.0438962
TCGATTA200.006801292852.7892645
CGGTGTG200.008517520549.8688621
TCCAACG200.008517520549.8688621
CTAACGC607.867129E-944.2700963
GCCCACG400.002266142535.46778548
GGTATCA14400.032.298991
GTATCAA30400.032.019711
AACTGTG10800.031.63285370
CGCCACG1101.488479E-830.96136764
TAGTACG450.005572129529.5155334
ATACGTA1053.3791366E-828.45934711
AATACGT1053.3791366E-828.45934710
TGCACAA1904.136673E-728.20514770
TATCAAC34800.027.7722642
AACGCAG35050.027.5681826
ATCAACG35150.027.487763
CAACGCA35250.027.3175685
TCAACGC35400.027.2018174
GAATACG1105.3039912E-827.1677079
Produced by FastQC (version 0.11.5)