FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689009

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689009
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1938730
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC62990.32490341615387397No Hit
GCCTTGGGCTGACCTAGGACGGTCACGAAGGTCCCTCCGCCGAACACCCAGTCACCCAGGCTGCTGTCATAGGA48670.2510406296905706No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG44170.22782955852542644No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT40630.20957018254217966No Hit
CTCCTGCACTGGGAGGAGTTCCAATATAGGGGCTGGTTTTGATGTTTTTTGGTATCAGCGACTTCCAGGAAAAGC36730.18945392086572138No Hit
GTTCCAATATAGGGGCTGGTTTTGATGTTTTTTGGTATCAGCGACTTCCA35090.18099477492997995No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACGAAGGTCCCTC34860.17980843129265034No Hit
GACCTAGGACGGTCACGAAGGTCCCTCCGCCGAACACCCAGTCACCCAGG33320.17186508693835656No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG32930.16985346077071073No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA32790.16913133855668402No Hit
GTCATAGGACTGGCAGTAATAATGAGCCTCATCCTCAGTCTGGAGCCCAG32300.16660391080759054No Hit
GGGCAGAGGGTCTCCATCTCCTGCACTGGGAGGAGTTCCAATATAGGGGCTGGTTTTGATGTTTTTTGGTATCAG31240.16113641404424547No Hit
GTGTGGGACTGGTCTGAGCTGAGGCCAGGCTGGAGGCTCAGGGGTATGGT31190.16087851325352162No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT30390.15675210060194045No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT30380.15670052044379568No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA28520.14710661102886943No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG28350.14622974834040842No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC28160.1452497253356579No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA27850.14365074043317017No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGAGGGGTC27800.14339283964244634No Hit
GTCTGGAGCCCAGTGATGGCCAGGGAGGCTAAAGTGCCAGACTTGGAGGC27490.14179385473995862No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG27060.13957590793973373No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC26980.13916326667457563No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC26820.13833798414425938No Hit
CAATAATACACGGCCGTGTCCGCAGCGGTCACAGAATTTAGTCTCAAAAA26730.1378737627209565No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC26590.1371516405069298No Hit
GGACAATCTCCAGCATGCCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGATCCTGGGCCCAGT26360.13596529686960018No Hit
CATTATTACTGCCAGTCCTATGACAGCAGCCTGGGTGACTGGGTGTTCGG26270.13550107544629733No Hit
CTATAGCACTGGGAGTACCAAGTACAAGTCTTCCCTCCAGGGTCGAGCCACCATTTCAATAGACACGTCCAAGAA26130.13477895323227063No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG25610.13209678500874283No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25310.13054938026439988No Hit
GGCCATCACTGGGCTCCAGACTGAGGATGAGGCTCATTATTACTGCCAGT24990.12889881520376742No Hit
GTCCTATGACAGCAGCCTGGGTGACTGGGTGTTCGGCGGAGGGACCTTCGTGACCGTCCTAGGTCAGCCCAAGG24750.12766089140829304No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG24710.127454570775714No Hit
GCTCCAGACTGAGGATGAGGCTCATTATTACTGCCAGTCCTATGACAGCA24340.1255461049243577No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT24060.12410186049630427No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG23840.12296709701711944No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT23520.12131653195648698No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23520.12131653195648698No Hit
CATGTATCCTGACTTCCATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGCCTCCAAGTCTGGCACTTTAGCCTC23290.12013018831915738No Hit
GTGATGGCCAGGGAGGCTAAAGTGCCAGACTTGGAGGCAGAGAATCGGTC23260.1199754478447231No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA23170.1195112264214202No Hit
GTATTATTGTGCGAGACTCCGGAGTGGTGGAAGGTTCGGGTTCGACCCCTGGGGCCAGGGAGCCCTGGTCACCGT22870.11796382167707725No Hit
CATCTATAGCACTGGGAGTACCAAGTACAAGTCTTCCCTCCAGGGTCGAG22460.1158490351931419No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC22430.11569429471870761No Hit
ATAATACACGGCCGTGTCCGCAGCGGTCACAGAATTTAGTCTCAAAAAGA22130.11414688997436466No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA21410.11043311858794158No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA21250.10960783605762535No Hit
CTCCAGGGTCGAGCCACCATTTCAATAGACACGTCCAAGAACCAATTCTTTTTGAGACTAAATTCTGTGACCG21100.10883413368545389No Hit
CAATAAACCCTCAATAATTGTTCTCATTGTCAATCAGAAATCTTGTTTTA20980.1082151717877167No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT20900.10780253052255857No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG20800.10728672894111094No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCTCCATCTCCTGCACTGGGAGGAG20780.1071835686248214No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC20690.10671934720151852No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC20680.10666776704337376No Hit
GTACAAGTCTTCCCTCCAGGGTCGAGCCACCATTTCAATAGACACGTCCA19820.10223187344292398No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC19620.10120027028002869No Hit
CAATTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG19440.1002718274334229No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAATA401.4897363E-552.93514669
TTACGGC200.00769493651.1703722
TATCGCG750.050.43395236
AGGGGGG4950.049.8875670
ATCGCGT704.5292836E-1044.2599237
AGTATCG1100.043.67301634
ACCGATC5200.042.0314827
CCGATCA5250.041.65161528
ACGAGAC5500.040.31604422
CGTATGC3100.040.16174341
TCGTATG2900.039.30147640
CGATCAG5700.038.38514729
CACGAGA5900.038.15799721
TATACAC5950.037.8353963
TCTCGTA3150.037.1981138
ATCTCCG6000.036.38312510
TATCTCG3900.036.1502136
TTATACA6150.036.0512852
CTTATAC6250.036.026731
GTGGTAT4550.035.9907381
Produced by FastQC (version 0.11.5)