FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689010

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689010
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1938730
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60780.3135042012038809No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC59340.30607665843103476No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG44100.22746849741841307No Hit
GCCTTGGGCTGACCTAGGACGGTCACGAAGGTCCCTCCGCCGAACACCCAGTCACCCAGGCTGCTGTCATAGGA43930.22659163472995208No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT36820.18991814228902426No Hit
CTCCTGCACTGGGAGGAGTTCCAATATAGGGGCTGGTTTTGATGTTTTTTGGTATCAGCGACTTCCAGGAAAAGC35340.18228427888359905No Hit
GTGTGGGACTGGTCTGAGCTGAGGCCAGGCTGGAGGCTCAGGGGTATGGT33830.17449567500373958No Hit
GTTCCAATATAGGGGCTGGTTTTGATGTTTTTTGGTATCAGCGACTTCCA33190.17119454488247463No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA30330.15644261965307185No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT29770.15355413079696503No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG29720.15329623000624118No Hit
GGGCAGAGGGTCTCCATCTCCTGCACTGGGAGGAGTTCCAATATAGGGGCTGGTTTTGATGTTTTTTGGTATCAG29150.15035616099198962No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG28560.1473129316614485No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACGAAGGTCCCTC28260.1457655269171055No Hit
GTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCTC28120.1450434047030788No Hit
GTCATAGGACTGGCAGTAATAATGAGCCTCATCCTCAGTCTGGAGCCCAG27530.1420001753725377No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27440.1415359539492348No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT27030.13942116746529945No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGAGGGGTC26840.13844114446054892No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA26750.13797692303724604No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC26200.13514001433928396No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC25530.13168414374358473No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC25430.13116834216213707No Hit
GACCTAGGACGGTCACGAAGGTCCCTCCGCCGAACACCCAGTCACCCAGG24690.12735141045942447No Hit
GTCTGGAGCCCAGTGATGGCCAGGGAGGCTAAAGTGCCAGACTTGGAGGC24550.12662928824539776No Hit
GTCCTATGACAGCAGCCTGGGTGACTGGGTGTTCGGCGGAGGGACCTTCGTGACCGTCCTAGGTCAGCCCAAGG24500.12637138745467394No Hit
GGACAATCTCCAGCATGCCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGATCCTGGGCCCAGT24410.12590716603137103No Hit
GGCCATCACTGGGCTCCAGACTGAGGATGAGGCTCATTATTACTGCCAGT24120.12441134144517287No Hit
CAATAATACACGGCCGTGTCCGCAGCGGTCACAGAATTTAGTCTCAAAAA23990.12374079938929092No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA23960.12358605891485663No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC23860.12307025733340898No Hit
GTATTATTGTGCGAGACTCCGGAGTGGTGGAAGGTTCGGGTTCGACCCCTGGGGCCAGGGAGCCCTGGTCACCGT23610.12178075337978987No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23530.12136811211463175No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG23440.12090389069132886No Hit
CATTATTACTGCCAGTCCTATGACAGCAGCCTGGGTGACTGGGTGTTCGG23340.12038808910988122No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG23270.12002702800286787No Hit
CTATAGCACTGGGAGTACCAAGTACAAGTCTTCCCTCCAGGGTCGAGCCACCATTTCAATAGACACGTCCAAGAA23080.11904700499811732No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT23070.11899542483997255No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG22950.11837646294223537No Hit
CTGCACAGGATCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCTC22230.1146626915558123No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA22080.11388898918364085No Hit
GCACAGGATCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCT21500.11089734001124447No Hit
GGGGTATGGTGGAGAGTTGGGGAAGGGGTGACTGGAAGAGGAAGGACCTTCCAGAGAGATGACTCGGGGAGG21420.11048469874608635No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCTCCATCTCCTGCACTGGGAGGAG21300.10986573684834919No Hit
CATGTATCCTGACTTCCATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGCCTCCAAGTCTGGCACTTTAGCCTC21080.10873097336916435No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT21030.10847307257844054No Hit
CATCTATAGCACTGGGAGTACCAAGTACAAGTCTTCCCTCCAGGGTCGAG21000.10831833210400624No Hit
CTCCAGGGTCGAGCCACCATTTCAATAGACACGTCCAAGAACCAATTCTTTTTGAGACTAAATTCTGTGACCG20950.10806043131328241No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC20920.10790569083884811No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC20610.10630670593636041No Hit
GCTCCAGACTGAGGATGAGGCTCATTATTACTGCCAGTCCTATGACAGCA20600.10625512577821564No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT20180.10408875913613552No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG19990.10310873613138496No Hit
CTCCTCATGTATCCTGACTTCCATCGGCCCTCAGGGGTCCCTGACCGATT19760.1019223924940554No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACGAAGGTCCCTCCGCCGAACAC19680.10150975122889727No Hit
ATAATACACGGCCGTGTCCGCAGCGGTCACAGAATTTAGTCTCAAAAAGA19680.10150975122889727No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)