FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689015

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689015
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences871512
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28720.329542220875903No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26750.30693782759158794No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20260.23246954717777837No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT19850.22776507953992603No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19060.2187003736035763No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18940.2173234562461561No Hit
GTAATAGACGGCCGTGTCCGCGGCGGTCACAGAACTCAGTTTCAGGGAGA18540.2127337317214221No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18300.20997989700658168No Hit
GTATCGTGGTTATCCCCGGACCTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT17590.20183313597517877No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17580.20171839286206042No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17000.19506329230119607No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16960.19460431984872267No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16950.19448957673560432No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT16630.19081779711581712No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT15990.18347423787624267No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14940.17142621099881586No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14650.1680986607183837No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13280.15237885422116965No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13270.1522641111080513No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13270.1522641111080513No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC13210.15157565242934118No Hit
CCACGATACTGTTGACAGTAATAAGTAGCAAAATCTTCAGGCTGGAGGCTGCTGATGGTGAGAGTGAAATCTGTC13200.15146090931622286No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13170.15111667997686778No Hit
ACTTATTACTGTCAACAGTATCGTGGTTATCCCCGGACCTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA13000.14916604705385583No Hit
GTCTATTACTGTGCGAGAGCGGGGCCCCATTACGATCTTTCGACTGGCTA12840.14733015724396223No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12510.14354363451105664No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT12390.14216671715363643No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11490.13183983697298488No Hit
GATCTATGCTGCATCCAGTCTGCAAAGTGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACAGATTTCA11460.13149560763362983No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11250.12908600225814448No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG11160.12805331424007932No Hit
GATTTCACTCTCACCATCAGCAGCCTCCAGCCTGAAGATTTTGCTACTTATTACTGTCAACAGTATCGTGGTTAT11050.12679113999577746No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC10970.12587319509083064No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10940.1255289657514756No Hit
ATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCA10640.12208667235792507No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG10430.11967706698243971No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10340.11864437896437455No Hit
GTCTCCATCCTCACTGTCTGCATCTGTAGGAGAGAGAGTCACCATCACCT10100.11589054424953414No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC9960.11428414066587723No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC9860.11313670953469372No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9790.11233350774286527No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9540.10946492991490651No Hit
ATGCTGCATCCAGTCTGCAAAGTGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCA9480.10877647123619641No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAAGGTC9430.10820275567060464No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9290.10659635208694773No Hit
GTAGGAGAGAGAGTCACCATCACCTGTCGGGCGAGTCAGGGCATCAGCAATTATTTAGCCTGGTATCAGCTGAGA9270.10636686586071105No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9240.10602263652135599No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9210.10567840718200094No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC9180.10533417784264588No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9140.10487520539017249No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9090.10430148982458072No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9040.10372777425898898No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT9000.10326880180651557No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT8980.10303931558027887No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG8830.1013181688835036No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC8800.10097393954414854No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8750.1004002239785568No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCTA200.007266097751.91546242
CACGACG200.007537475351.4362437
TTCGATC200.007727822351.11279731
TCGAAAT200.00778437751.01861229
TACTGAA5450.048.39879670
TCGTATG2600.039.76951240
ATGCCGT2550.039.4810444
CGTATGC2550.039.27788541
GTCTACT4300.037.5260267
TCTACTG4600.037.49526668
GTGGTAT1900.037.4675251
CTTATAC3200.037.077241
CCCACGA3200.036.01375619
TATACAC3600.035.7763183
ATCTCCG3450.035.36495210
GTATCAA24550.035.3490561
CGAGACA3500.033.9167123
CACGAGA3400.033.9050321
TTGTCTA4800.033.6296865
TGTCTAC4850.033.57283866
Produced by FastQC (version 0.11.5)