FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689016

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689016
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences871512
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37390.429024499949513No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27480.3153140748492276No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23240.2666629948870469No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21750.24956627103241263No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20090.23051891425476642No Hit
GTATCGTGGTTATCCCCGGACCTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT17930.2057344018212027No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17230.19770238390291814No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17150.19678443899797132No Hit
GTAATAGACGGCCGTGTCCGCGGCGGTCACAGAACTCAGTTTCAGGGAGA16610.1905883108895804No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16310.1871460174960299No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15480.1776223391072068No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14740.16913134873644883No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14700.1686723762839754No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14630.16786917449214697No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14230.16327944996741295No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14180.1627057344018212No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13930.15983715657386244No Hit
GTCTATTACTGTGCGAGAGCGGGGCCCCATTACGATCTTTCGACTGGCTA13420.15398525780482655No Hit
CCACGATACTGTTGACAGTAATAAGTAGCAAAATCTTCAGGCTGGAGGCTGCTGATGGTGAGAGTGAAATCTGTC12600.1445763225291218No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12540.1438878638504117No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT12510.14354363451105664No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT12440.14274043271922818No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12440.14274043271922818No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12380.1420519740405181No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12280.14090454290933457No Hit
ACTTATTACTGTCAACAGTATCGTGGTTATCCCCGGACCTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA12090.13872442376008592No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12070.13849493753384923No Hit
GATCTATGCTGCATCCAGTCTGCAAAGTGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACAGATTTCA11180.128282800466316No Hit
GATTTCACTCTCACCATCAGCAGCCTCCAGCCTGAAGATTTTGCTACTTATTACTGTCAACAGTATCGTGGTTAT11110.12747959867448755No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10980.12598793820394902No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC10880.1248405070727655No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10530.12082449811362322No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10530.12082449811362322No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC10290.1180706633987828No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9960.11428414066587723No Hit
GTCTCCATCCTCACTGTCTGCATCTGTAGGAGAGAGAGTCACCATCACCT9870.11325145264781207No Hit
GGTCACAGGAGCTGCCCCGCGAGAAGTACCTGACTTGGGCATCCCGGCAG9860.11313670953469372No Hit
GTCTGCATCTGTAGGAGAGAGAGTCACCATCACCTGTCGGGCGAGTCAGG9700.1113008197248001No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC9470.10866172812307805No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9360.10739955387877619No Hit
GTAATAAGTAGCAAAATCTTCAGGCTGGAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCG8890.10200662756221371No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG8860.10166239822285866No Hit
ATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCA8850.10154765510974031No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC8850.10154765510974031No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8730.10017073775232009No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)