FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689021

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689021
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences673454
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCTGCGGCGGTCACAGAGGTCAGCTTCAGGGAGA58540.8692501640795065No Hit
GGGTATGAGTAGGTGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTATTCTGCATC31760.4715986541025816No Hit
GTATATTACTGTGCGAGCCTGAATTTGGGGATGTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGT29700.4410100764120489No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24550.3645386321857172No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21060.3127162360012711No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG16750.2487178040370983No Hit
GGCTAACAGTTTCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCACACGAACTGTGGCTGCACCAT16670.2475298981073689No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16090.23891758011683056No Hit
GATCTATTCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCA15280.2268900325783201No Hit
GTCTATTATAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGT14800.21976259699994358No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGTATGAGTAGGTGGTTAGCCTGGTATCAGCAGAAA14630.21723829689926857No Hit
ACTCAGGACACAGCATGGACATGATGGTCCCCGCTCAGCTCCTGGGGCTC14510.2154564380046744No Hit
CAGTAATATACAGCCGTGTCTGCGGCGGTCACAGAGGTCAGCTTCAGGGA14360.21322911438643172No Hit
GTCTCTGGTGACTCCATCAGCAGTAGTAGTTACTACTGGGGCTGGGTCCG14080.20907144363237876No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13990.20773504946143317No Hit
CTATTATAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCCGTAGACACGTCCAAGAA13880.2061016788080552No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12570.18664971920873585No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12450.18486786031414174No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12420.1844223955904932No Hit
CGTCCATACCGTACATCCCCAAATTCAGGCTCGCACAGTAATATACAGCC12020.17848286594184606No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11000.1633370653377959No Hit
CTGTTAGCCTGTTGACAATAGTAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC10930.16229764764928264No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10800.16036730051347234No Hit
CTATAATAGACACTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGACCCAGCCCCAGTAGTAACTACTA10740.15947637106617527No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10660.15828846513644584No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10660.15828846513644584No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10500.155912653276987No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT10480.15561567679455462No Hit
GGACACAGCATGGACATGATGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT10380.15413079438239286No Hit
ATAATAGACACTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGACCCAGCCCCAGTAGTAACTACTA10230.1519034707641502No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG10170.15101254131685313No Hit
GTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGGGAGTGCATCCGCCCCAACCCTTTTCCCC10030.14893370593982663No Hit
ACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCCGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGA9920.14730033528644867No Hit
GCATGGACATGATGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG9770.145073011668206No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTACTATTGTCAACAGGCTAACAGTTTC9700.14403359397969273No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTGTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCACGGGAAA9640.14314266453239569No Hit
GGATTGGGAGTGTCTATTATAGTGGGAGCACCTACTACAACCCGTCCCTC9290.13794557608982944No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT9210.13675767016010004No Hit
ATTCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCA9100.13512429950672206No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9100.13512429950672206No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA8870.13170906995874995No Hit
GAATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC8650.12844232865199404No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT8360.12413616965672489No Hit
GTCCCTCACCTGCGCTGTCTCTGGTGACTCCATCAGCAGTAGTAGTTACT8110.12042396362632043No Hit
GCCACAGTTCGTGTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCACGG8070.11983001066145572No Hit
CTCATACCCTGACTCGCCCGACAAGTGATGGTGACTCTGTCTCCTACAGA8060.11968152242023954No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG7760.1152268751837542No Hit
CAGCATGGACATGATGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT7750.11507838694253802No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7680.11403896925402476No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC7670.1138904810128086No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7640.11344501628916005No Hit
ATATTACTGTGCGAGCCTGAATTTGGGGATGTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTC7570.11240559860064682No Hit
GTGCAGCCACAGTTCGTGTGATTTCCACCTTGGTCCCTTGGCCGAACGTC7540.11196013387699828No Hit
GACCCACTCAGGACACAGCATGGACATGATGGTCCCCGCTCAGCTCCTGG7540.11196013387699828No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT7470.11092071618848505No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7430.11032676322362032No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7410.11002978674118796No Hit
GTCATGGATCTCATGTGCAAGAAAATGAAGCACCTGTGGTTCTTCCTCCTGCTGGTGGCGGCTCCCAGATGGGTC7350.1091388572938909No Hit
GAGTAGGTGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACC7260.1078024631229453No Hit
CTACTGGGGCTGGGTCCGCCAGCCCCCAGGGAAGGGGCTGGAGTGGATTGGGAGTGTCTATTATAGTGGGAGCAC7240.10750548664051295No Hit
GCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGGGTTG7190.10676304543443203No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTG7160.10631758071078352No Hit
GTATCAACGCAGAGTACGGGATGCTTTCTGAGAGTCATGGATCTCATGTGCAAGAAAATGAAGCACCTGTGGTT7150.10616909246956735No Hit
CCCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATACCGT7140.10602060422835115No Hit
CCTGTGAGGTGGCTGCGTACTTGCCCCCTCTCAGGACTGATGGGAAGCCC7050.10468421005740555No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7010.10409025709254083No Hit
CTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGACCCAGCCCCA6930.10290235116281142No Hit
GTGCTGGACTTTGCACACCACGTGTTCGTCTGTGCCCTGCATGACGTCCT6900.10245688643916288No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG6830.10141746875064965No Hit
GTCTCCATCTTCCGTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT6820.10126898050943345No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)