FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689022

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689022
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences673454
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCTGCGGCGGTCACAGAGGTCAGCTTCAGGGAGA52710.7826815194504746No Hit
GGGTATGAGTAGGTGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTATTCTGCATC26730.39690906877084403No Hit
GTATATTACTGTGCGAGCCTGAATTTGGGGATGTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGT24440.36290526153233926No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23080.34271086072693907No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG18330.2721789461492544No Hit
GGCTAACAGTTTCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCACACGAACTGTGGCTGCACCAT16270.24159036845872178No Hit
CTGTTAGCCTGTTGACAATAGTAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC15670.23268107398575105No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15460.22956282092021132No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14660.21768376162291705No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14490.21515946152224205No Hit
GTCTCTGGTGACTCCATCAGCAGTAGTAGTTACTACTGGGGCTGGGTCCG14380.21352609086886407No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14370.21337760262764793No Hit
ACTCAGGACACAGCATGGACATGATGGTCCCCGCTCAGCTCCTGGGGCTC13570.20149854333035366No Hit
CTATTATAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCCGTAGACACGTCCAAGAA13460.1998651726769757No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13100.1945195959931933No Hit
GATCTATTCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCA13060.1939256430283286No Hit
GTCTATTATAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGT12970.192589248857383No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12140.1802647248364402No Hit
CAGTAATATACAGCCGTGTCTGCGGCGGTCACAGAGGTCAGCTTCAGGGA11770.1747706599114416No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11510.17090996563982097No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA11350.16853415378036216No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGTATGAGTAGGTGGTTAGCCTGGTATCAGCAGAAA11130.1652674124736062No Hit
GGACACAGCATGGACATGATGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT10480.15561567679455462No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10130.1504185883519884No Hit
CGTCCATACCGTACATCCCCAAATTCAGGCTCGCACAGTAATATACAGCC10110.15012161186955605No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT10080.14967614714590752No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10050.14923068242225898No Hit
GTCCCTCACCTGCGCTGTCTCTGGTGACTCCATCAGCAGTAGTAGTTACT10020.14878521769861044No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10010.14863672945739426No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC9820.14581545287428688No Hit
ACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCCGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGA9720.1443305704621251No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9620.14284568804996334No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9540.1416577821202339No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTACTATTGTCAACAGGCTAACAGTTTC9510.14121231739658535No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9360.1389849937783427No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTGTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCACGGGAAA9170.13616371719523532No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8960.13304546412969556No Hit
GTGCAGCCACAGTTCGTGTGATTTCCACCTTGGTCCCTTGGCCGAACGTC8880.13185755819996614No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8870.13170906995874995No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG8750.12992721106415583No Hit
CTATAATAGACACTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGACCCAGCCCCAGTAGTAACTACTA8590.127551399204697No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8560.12710593448104843No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT8550.12695744623983227No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG8520.12651198151618373No Hit
GCATGGACATGATGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG8250.12250279900334693No Hit
GTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGGGAGTGCATCCGCCCCAACCCTTTTCCCC8100.12027547538510425No Hit
GCCACAGTTCGTGTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCACGG8000.11879059297294246No Hit
ATAATAGACACTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGACCCAGCCCCAGTAGTAACTACTA7940.1178996635256454No Hit
CTCATACCCTGACTCGCCCGACAAGTGATGGTGACTCTGTCTCCTACAGA7930.11775117528442923No Hit
GCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGGGTTG7920.11760268704321304No Hit
ATTCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCA7910.11745419880199687No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG7830.11626629287226743No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC7660.11374199277159243No Hit
GGATTGGGAGTGTCTATTATAGTGGGAGCACCTACTACAACCCGTCCCTC7620.1131480398067277No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT7580.112554086841863No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT7540.11196013387699828No Hit
GAATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC7420.11017827498240414No Hit
GATGGGTCCTGTCCCAGCTGCAGCTGCAGGAGTCGGGCCCAGGACTGGTGAAGCCTTCGGAGACCCTGTCCCTCA7380.10958432201753944No Hit
GGACTGGTGAAGCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTCTGGTGACTCCATCAGCAGTAGTAGTTA7380.10958432201753944No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC7320.10869339257024235No Hit
GTCATGGATCTCATGTGCAAGAAAATGAAGCACCTGTGGTTCTTCCTCCTGCTGGTGGCGGCTCCCAGATGGGTC7290.10824792784659383No Hit
GGTGAAGCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTCTGGTGACT7220.10720851015808058No Hit
CCCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATACCGT7220.10720851015808058No Hit
GTATCAACGCAGAGTACGGGATGCTTTCTGAGAGTCATGGATCTCATGTGCAAGAAAATGAAGCACCTGTGGTT7170.1064660689519997No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7050.10468421005740555No Hit
ATATTACTGTGCGAGCCTGAATTTGGGGATGTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTC7010.10409025709254083No Hit
CAGCATGGACATGATGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT7000.10394176885132465No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG6850.101714445233082No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACT6830.10141746875064965No Hit
GTCCTGTCCCAGCTGCAGCTGCAGGAGTCGGGCCCAGGACTGGTGAAGCC6750.10022956282092021No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)