FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689023

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689023
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences492560
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18180.36909209030371937No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT16370.3323452980347572No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12460.25296410589572843No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12230.24829462400519733No Hit
GCTTTAGCCTGGTATCAGCACAAACCGGGGAATGCTCCTAAGTTGCTGAT12100.24565535163228844No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11420.23184992691245737No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10890.2210898164690596No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10470.21256293649504632No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10090.20484814032808185No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10000.20302095176222187No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9740.1977424070164041No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT9610.1951031346434952No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTT9590.19469709273997077No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTT9520.19327594607763524No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9150.185764170862433No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG8970.182109793730713No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT8550.1735829137566997No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG8110.16464999187916193No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG7720.15673217476043527No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7570.15368686048400196No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCT7020.14252070813707973No Hit
CTTTAATAATTACCCTCTACTCACTTTCGGCGGAGGGACCAGGGTGGAGATCAAGAGAACTGTGGCTGCACCAT6940.14089654052298195No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT6910.1402874776676953No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6850.13906935195712197No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6620.13439987006659088No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT6520.13236966054896865No Hit
AGTCAGGACATTCGCAGTGCTTTAGCCTGGTATCAGCACAAACCGGGGAA6360.1291213253207731No Hit
ATAGGAGACAGAGTCACCATTACTTGCCGGGCAAGTCAGGACATTCGCAGTGCTTTAGCCTGGTATCAGCACAAA6340.12871528341724867No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA6300.12790319961019977No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCTGCTCTGG6230.12648205294786422No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA6160.12506090628552868No Hit
CACCATCACCAGCCTGCAGCCTGAAGATTTTGCAACTTATTATTGTCAACACTTTAATAATTACCCTCTACTCA6080.12343673867143089No Hit
GATTTCACTCTCACCATCACCAGCCTGCAGCCTGAAGATTTTGCAACTTATTATTGTCAACACTTTAATAATTAC6060.12303069676790646No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC6040.12262465486438201No Hit
TTATTAAAGTGTTGACAATAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTC5900.11978236153971089No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG5900.11978236153971089No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCTCTTGATCTCCACCCTGGTCC5760.1169400682150398No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT5680.11531590060094202No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC5600.11369173298684424No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG5590.11348871203508203No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT5590.11348871203508203No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT5580.11328569108331979No Hit
GAGTGAAATCTGTCCCAGATCCACTGGCACTGAACCTTAATGGGACGCCA5540.11247360727627091No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC5510.11186454442098424No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC5410.10983433490336203No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC5360.10881923014455092No Hit
CCATTAAGGTTCAGTGCCAGTGGATCTGGGACAGATTTCACTCTCACCAT5350.10861620919278868No Hit
GGCTAAAGCACTGCGAATGTCCTGACTTGCCCGGCAAGTAATGGTGACT5290.10739808348221537No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT5290.10739808348221537No Hit
CAAGAGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCT5270.10699204157869092No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC5240.10638297872340426No Hit
GTGATACAAGGCCGTGTCCTCAACTCTCAGACTGTTCATTTGCAGATATA5230.10617995777164202No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT5180.10516485301283092No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG5170.10496183206106871No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG5150.10455579015754426No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCAT5130.1041497482540198No Hit
CCATCTTCCCTGTCTGCATCTATAGGAGACAGAGTCACCATTACTTGCCG5110.10374370635049537No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG5060.10272860159168426No Hit
GCACTGAACACAGAGGACTCACCATGGAGTCGGGACTGAGCTGGATTTTC5040.10232255968815981No Hit
CCATTACTTGCCGGGCAAGTCAGGACATTCGCAGTGCTTTAGCCTGGTAT5030.10211953873639759No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT1400.056.38903837
ATGCCGT1750.051.6103444
GTAACAT200.007506971751.48564537
TTATACA1850.051.348112
CGTATGC1750.051.32750341
TCGTATG1750.051.25860240
GAGACCG1750.050.44596524
GTATGCC1800.050.02588742
GATCTCG1550.048.667636
GCCGTCT1900.047.71443646
TGCCGTC1900.047.60513745
CTTATAC1950.046.975061
AGACCGA1900.046.47755425
CACGAGA1900.046.44450821
CGAGGCT1950.045.39658429
CCGTCTT2000.045.3808147
ACGAGAC1950.045.2582222
TATACAC2100.045.235243
GGCTGAT2000.044.39739632
ACCGAGG2000.044.2031127
Produced by FastQC (version 0.11.5)