FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689024

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689024
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences492560
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21730.44116452817930807No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT14930.30311028098099724No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14220.28869579340587953No Hit
GCTTTAGCCTGGTATCAGCACAAACCGGGGAATGCTCCTAAGTTGCTGAT12590.2556033782686373No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12330.25032483352281953No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12300.24971577066753287No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10620.2156082507714796No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10500.21317199935033299No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9380.1904336527529641No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9340.18962156894591523No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG9330.189418547994153No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTT9100.18474906610362188No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG8930.18129770992366412No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8820.1790644794542797No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT8640.17541010232255969No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA8400.17053759948026637No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTT8250.16749228520383305No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG7520.15267175572519084No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT7480.15185967191814195No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT7360.1494234204969953No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG7280.14779925288289752No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCT7140.1449569595582264No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC7140.1449569595582264No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG6790.13785122624654864No Hit
CTTTAATAATTACCCTCTACTCACTTTCGGCGGAGGGACCAGGGTGGAGATCAAGAGAACTGTGGCTGCACCAT6640.13480591197011532No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT6590.1337908072113042No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA6580.13358778625954199No Hit
CACCATCACCAGCCTGCAGCCTGAAGATTTTGCAACTTATTATTGTCAACACTTTAATAATTACCCTCTACTCA6490.131760597693682No Hit
ATAGGAGACAGAGTCACCATTACTTGCCGGGCAAGTCAGGACATTCGCAGTGCTTTAGCCTGGTATCAGCACAAA6350.1289183043690109No Hit
AGTCAGGACATTCGCAGTGCTTTAGCCTGGTATCAGCACAAACCGGGGAA6170.12526392723729088No Hit
GATTTCACTCTCACCATCACCAGCCTGCAGCCTGAAGATTTTGCAACTTATTATTGTCAACACTTTAATAATTAC6130.124451843430242No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC5940.1205944453467598No Hit
GAGTGAAATCTGTCCCAGATCCACTGGCACTGAACCTTAATGGGACGCCA5690.11551892155270424No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG5590.11348871203508203No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC5550.11267662822803312No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA5500.11166152346922201No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG5490.11145850251745981No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT5450.11064641871041091No Hit
GGGTGGAGATCAAGAGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG5330.10821016728926425No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCTGCTCTGG5280.10719506253045313No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC5280.10719506253045313No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT5230.10617995777164202No Hit
TTATTAAAGTGTTGACAATAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTC5180.10516485301283092No Hit
CCATTAAGGTTCAGTGCCAGTGGATCTGGGACAGATTTCACTCTCACCAT5120.1039467273022576No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT5110.10374370635049537No Hit
CCATCTTCCCTGTCTGCATCTATAGGAGACAGAGTCACCATTACTTGCCG5100.10354068539873316No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC5050.10252558063992205No Hit
GCACTGAACACAGAGGACTCACCATGGAGTCGGGACTGAGCTGGATTTTC5050.10252558063992205No Hit
GTCTGAGAGTTGAGGACACGGCCTTGTATCACTGTGCAAGAGACATGGAC5020.10191651778463538No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC5010.10171349683287315No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG5000.10151047588111094No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGGTA405.5394215E-542.3795051
GTGGTAT2000.040.6843261
ATCTTAC350.001467613238.746981
ATAATAT451.11225316E-437.651553
GACAGGT558.3242885E-636.9857521
GTATCAA13250.034.799171
GTGAGCG1651.3375575E-732.0779470
ATTTAAT1053.1652238E-431.50512170
TCCTATA652.619598E-531.2829274
TATCAAC15150.029.9782122
TCCGAGA2002.1682354E-829.77233970
ATTCACT1052.7630449E-829.051382
TTGCCTA605.477502E-428.6960539
GCGTAGA8900.028.24841170
ATCAACG16450.027.8095043
TCAACGC16400.027.6904754
CAACGCA16450.027.6063125
AACGCAG16450.027.6063126
GTCATAG3450.027.515971
GTGTATA500.00841715727.1228851
Produced by FastQC (version 0.11.5)