FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689028

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689028
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1392596
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG123110.8840324114100571No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT57350.41182080086399786No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC46490.3338369491223585No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC43470.3121508319713686No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA39490.2835711146664216No Hit
CTCCTGCACGGCCAGTCAGAGTGTTAGCAGCTACTTAGCCTGGTATCAACAGAAACCTGGCCAGGCTCCCAGGCT34600.24845683888220274No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT33880.24328663876673493No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC33830.24292759709204967No Hit
GTCTTTGTCTCCGGGGGAAAGAGCCACCCTCTCCTGCACGGCCAGTCAGA32530.2335925135502328No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32500.23337708854542163No Hit
GTCTGGGACAGACTTCACTCTCACCCTCAGCAACGTAGAGCCTGAAGATT32030.23000209680338018No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30710.2205233965916892No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA29890.21463511312685085No Hit
GTCTCCGGCCACCCTGTCTTTGTCTCCGGGGGAAAGAGCCACCCTCTCCTGCACGGCCAGTCAGAGTGTTAGCAG29430.21133192971974643No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28860.2072388546283344No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT28620.20551545458984516No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT28400.20393567122123No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26470.1900766625783788No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG25490.18303944575454761No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24950.17916179566794677No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA23930.17183734550436738No Hit
GTTTATTACTGTCAGAGTCGGGGCTCCTTCGGCCAAGGGACACGACTGGA23590.1693958621165076No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23360.16774427041295537No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22930.16465651201066211No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT22880.16429747033597683No Hit
GGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTTGGATGCAT22390.16077886192406127No Hit
GTCAGAGTGTTAGCAGCTACTTAGCCTGGTATCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATG22130.15891184521569787No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTACGTTGCTGAGGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA21940.15754748685189388No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT21640.15539323680378228No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC21440.15395707010504123No Hit
CTCTCACCCTCAGCAACGTAGAGCCTGAAGATTTTGCAGTTTATTACTGT21270.15273632841111134No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20780.14921771999919572No Hit
GACTTCACTCTCACCCTCAGCAACGTAGAGCCTGAAGATTTTGCAGTTTA19990.14354486153916857No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18840.1352869030214075No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18810.13507147801659633No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT18560.13327626964317002No Hit
GTGTTAGCAGCTACTTAGCCTGGTATCAACAGAAACCTGGCCAGGCTCCC17410.12501831112540895No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17390.12487469445553484No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17050.12243321106767503No Hit
ACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTT16970.12185874438817863No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16790.12056619435931167No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACACAGTC16770.12042257768943757No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16710.11999172767981525No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16320.1171912026172702No Hit
TCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT16250.11668854427271082No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16150.1159704609233403No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGAGCCCCGACTCTGACAGTAATAAACTG15830.1136725942053546No Hit
ACCCTCTCCTGCACGGCCAGTCAGAGTGTTAGCAGCTACTTAGCCTGGTA15740.11302631919092113No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA15700.11273908585117291No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA15660.11245185251142471No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC15540.11159015249218007No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15450.1109438774777466No Hit
GTGTTGACACAGTCTCCGGCCACCCTGTCTTTGTCTCCGGGGGAAAGAGC15330.11008217745850198No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15250.10950771077900553No Hit
GTCGTGTCCCTTGGCCGAAGGAGCCCCGACTCTGACAGTAATAAACTGCA15170.10893324409950914No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15030.10792792741039037No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT15030.10792792741039037No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC14990.10764069407064217No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14970.10749707740076805No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG14870.10677899405139753No Hit
GAGTGTTAGCAGCTACTTAGCCTGGTATCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATC14810.10634814404177521No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14680.10541463568759352No Hit
CAGCAACGTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGAGTCGGGGCTCCTTCGGCCAAGGGACACGA14620.10498378567797122No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14510.10419389399366363No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14430.1036194273141672No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA14130.10146517726605563No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14090.10117794392630743No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13960.10024443557212573No Hit
CATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA13950.10017262723718869No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGT150.002657268466.934759
TATATCG252.9820477E-453.5516475
ATATCGC200.00829633350.204676
CCTAACG352.7147176E-547.827712
CGCTATC350.001565297138.24705512
CTAACGC601.6512406E-533.4733853
CGGCTGA5750.032.83484669
TGTCGGC4900.032.3122966
GACAGGT2950.031.7964741
ATCAACG50550.030.8577613
GTCGGCT5100.030.78664467
TCAACGC50900.030.5787144
CAACGCA51700.030.1681185
AACGCAG51900.030.0518636
CGCAGAG53850.028.8994068
AGAGTAC54550.028.77293611
ACGCAGA54300.028.7236047
TTGTCGG5400.028.42226865
TAGGCGG5150.027.98446847
GCAGAGT55550.027.9547489
Produced by FastQC (version 0.11.5)