FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689029

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689029
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences827427
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAAATACA46400.5607745456698899No Hit
CCTATGTGCTGACTCAGCCACCCTCGGTATCAGTGGCCCCAGGAGAGACG22970.27760757142321923No Hit
GTATATTACTGTGCGAAAAACCACTATAGGAGCAGTTGGTACTTTGAAAA21890.26455506044642No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT21170.25585338646188727No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG21140.2554908167125317No Hit
GGATTACCTGTGGGGGTACCAACATTGGAAGTAGAAGTGTGTTTTGGTACCAGAAGAAGCCAGGCCAGGCCC18570.22443067485107449No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC18020.21778356277955638No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT17940.21681671011460832No Hit
GTGTTACTGATCATCTATTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCA17660.21343272578728997No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGATGATCAGTAACACTATCCCACACCTGACAGTAATAGT17410.2104113112093272No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA16310.19711708706629105No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG16180.1955459514857504No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT16030.19373310273897273No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTGTCTGCCTTCCAG15770.19059083157789147No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTC15530.1876902735830472No Hit
CCCTTAGCACCTATGCCACGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG15490.18720684725057316No Hit
CCCCTGTGCTGGTCGTCTATGCTGATAGCGGCCGGCCCTCGGGGATCCCT14920.18031802201281805No Hit
CAGTAACACTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGG14910.18019716542969955No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGATGATCAGTAACACTATCCCACACCTGA14780.1786260298491589No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT14620.17669232451926273No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC13620.16460666620741166No Hit
CTCCTGTGCAGCCTCTGGATTCACCCTTAGCACCTATGCCACGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG13130.15868469363460463No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT12920.15614670538911588No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC12870.15554242247352335No Hit
ATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGC12290.1485327406526497No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12030.14539046949156845No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT11950.14442361682662036No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACT11910.14394019049414633No Hit
CTACGCAGACTCCGTGAGGGGCCGGTTCACCATCTCCAAAGACAATTCCAAGAACACGCTGTATTTGCAAATGAA11710.1415230588317761No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG11540.1394684969187614No Hit
CACTACGCAGACTCCGTGAGGGGCCGGTTCACCATCTCCAAAGACAATTC11400.13777650475510225No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11210.13548022967585055No Hit
CAGTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAAATA11090.13402995067842843No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG11020.13318395459659885No Hit
GCACAGGGGCCTGGCCTGGCTTCTTCTGGTACCAAAACACACTTCTACTT11000.13294224143036185No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC10940.13221710193165076No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG10510.12702026885755482No Hit
CCCCAGGAGAGACGGCCAGGATTACCTGTGGGGGTACCAACATTGGAAGT10490.1267785556913178No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA10480.12665769910819927No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTGTCTGCCTTCCAGGCCACTGTCACGGCTC10360.12520742011077715No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG10340.12496570694454012No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC10230.12363628453023649No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT10160.12279028844840692No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT10120.12230686211593289No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT10020.12109829628474778No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC9960.12037315678603672No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGTTGGAGT9820.11868116462237757No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC9750.11783516854054797No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG9710.11735174220807396No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT9590.11590146321065183No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC9580.1157806066275333No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG9530.11517632371194075No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA9480.11457204079634821No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC9470.11445118421322968No Hit
CGGCCACCCTGACCATCAGCAGGGTCGAAGCCGGGGATGAGGCCGACTATTACTGTCAGGTGTGGGATAGTGTTA9460.11433032763011118No Hit
GGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTATCAGTGGCCCCAGGAGAGACGGCCAGGATTAC9450.11420947104699265No Hit
GGCCAGGCCCCTGTGCTGGTCGTCTATGCTGATAGCGGCCGGCCCTCGGG9180.11094634330279288No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT9050.10937520772225223No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG8900.10756235897547456No Hit
GTGTGGGATAGTGTTACTGATCATCTATTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTCAGCCCAAGGCTGCC8850.10695807605988202No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT8780.10611207997805244No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGC8640.1044200878143933No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCCTTAGCACCTATGCCACGAGCTGGGTCCG8630.10429923123127478No Hit
CCCTGAGGCGACGGTGACCAGGGTTCCCTGGCCCCAGTTTTCAAAGTACC8610.10405751806503777No Hit
GGGTTCCCTGGCCCCAGTTTTCAAAGTACCAACTGCTCCTATAGTGGTTTTTCGCACAGTAATATACGGCCGTGT8590.10381580489880074No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC8500.10272809565073414No Hit
GTACCGGCCTGGGGCCTGGGGCTCAGGCATTGGGGCGCTGGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCT8430.10188209956890457No Hit
CGGTATCAGTGGCCCCAGGAGAGACGGCCAGGATTACCTGTGGGGGTACC8430.10188209956890457No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC8350.1009152469039565No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCAC8330.10067353373771946No Hit
GACCTGCCCTCGCCACTGAAGCAGACCATCTCCCGGCCCAAGGGGGTGGCCCTGCACAGGCCCGATGTCTACTTG8290.10019010740524542No Hit
GTGTGAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGC8280.10006925082212689No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1450.052.4802441
TATCTCG1400.051.6886436
CGAGACG1650.049.91028223
GACGGAC1600.049.3343426
AGGGGGG1950.049.27298470
TCTCGTA1500.048.30135738
TCGTATG1600.047.53125840
CGGACTC1600.047.22076428
ATCTCGT1550.046.70919437
ACGGACT1700.046.44074227
TGCCGTC1750.045.6213545
TATACAC1900.045.1545643
CGAGCCC1750.045.10568215
CACGAGA1800.043.844821
ACGAGAC1800.043.844822
CTTATAC1900.043.353621
GTGGTAT2050.041.855631
ATGCCGT1900.040.1583544
TACACAT2000.039.4650885
TTATACA2100.039.222332
Produced by FastQC (version 0.11.5)