FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689030

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689030
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences827427
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAAATACA43610.5270555589798254No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG23640.28570496249215943No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT21180.25597424304500577No Hit
GTATATTACTGTGCGAAAAACCACTATAGGAGCAGTTGGTACTTTGAAAA20980.2535571113826356No Hit
CCTATGTGCTGACTCAGCCACCCTCGGTATCAGTGGCCCCAGGAGAGACG20330.24570143347993237No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA19010.22974836450828895No Hit
GTGTTACTGATCATCTATTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCA18910.22853979867710383No Hit
GGATTACCTGTGGGGGTACCAACATTGGAAGTAGAAGTGTGTTTTGGTACCAGAAGAAGCCAGGCCAGGCCC18760.22672694993032616No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTGTCTGCCTTCCAG17590.21258672970546041No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGATGATCAGTAACACTATCCCACACCTGACAGTAATAGT17160.20738989663136448No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC16680.20158878064167593No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT16460.1989299358130687No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC15600.18853626966487677No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG14670.17729660743485529No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14560.17596718502055167No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGATGATCAGTAACACTATCCCACACCTGA14490.17512118893872208No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC14110.17052863878021868No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTC14090.17028692561398165No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT14080.17016606903086315No Hit
CAGTAACACTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGG13650.16496923595676719No Hit
CCCTTAGCACCTATGCCACGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG13330.16110182529697484No Hit
CCCCTGTGCTGGTCGTCTATGCTGATAGCGGCCGGCCCTCGGGGATCCCT13310.1608601121307378No Hit
CTCCTGTGCAGCCTCTGGATTCACCCTTAGCACCTATGCCACGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG13310.1608601121307378No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT12420.15010387623319035No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT12300.1486535972357682No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG12100.14623646557339803No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12000.1450278997422129No Hit
GCACAGGGGCCTGGCCTGGCTTCTTCTGGTACCAAAACACACTTCTACTT10710.12943740051992503No Hit
CTACGCAGACTCCGTGAGGGGCCGGTTCACCATCTCCAAAGACAATTCCAAGAACACGCTGTATTTGCAAATGAA10690.129195687353688No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10470.12653684252508077No Hit
CCCCAGGAGAGACGGCCAGGATTACCTGTGGGGGTACCAACATTGGAAGT10470.12653684252508077No Hit
CAGTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAAATA10400.12569084644325118No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT10230.12363628453023649No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC10150.12266943186528842No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC10120.12230686211593289No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACT10120.12230686211593289No Hit
ATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGC9970.12049401336915522No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA9930.12001058703668117No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGTTGGAGT9880.11940630412108863No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT9870.11928544753797013No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC9820.11868116462237757No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA9810.11856030803925904No Hit
GGGTTCCCTGGCCCCAGTTTTCAAAGTACCAACTGCTCCTATAGTGGTTTTTCGCACAGTAATATACGGCCGTGT9750.11783516854054797No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT9700.11723088562495543No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG9690.11711002904183693No Hit
CACTACGCAGACTCCGTGAGGGGCCGGTTCACCATCTCCAAAGACAATTC9640.11650574612624437No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC9630.11638488954312586No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG9580.1157806066275333No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC9560.11553889346129628No Hit
GTGTGGGATAGTGTTACTGATCATCTATTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTCAGCCCAAGGCTGCC9510.11493461054570372No Hit
GGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTATCAGTGGCCCCAGGAGAGACGGCCAGGATTAC9490.11469289737946671No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG9410.11372604471451862No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCCTTAGCACCTATGCCACGAGCTGGGTCCG9310.11251747888333351No Hit
GTGTGAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGC9310.11251747888333351No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTGTCTGCCTTCCAGGCCACTGTCACGGCTC9280.11215490913397798No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC9130.1103420603872003No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGC9050.10937520772225223No Hit
GTACCGGCCTGGGGCCTGGGGCTCAGGCATTGGGGCGCTGGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCT9030.1091334945560152No Hit
CCCTGAGGCGACGGTGACCAGGGTTCCCTGGCCCCAGTTTTCAAAGTACC8910.10768321555859309No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT8910.10768321555859309No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG8890.10744150239235606No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACAC8860.10707893264300053No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC8820.10659550631052649No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG8800.10635379314428946No Hit
GGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCCGAGGGCCGGCCGCTATCAGCATAGA8770.10599122339493394No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGA8770.10599122339493394No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT8740.10562865364557841No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT8710.10526608389622287No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCAC8540.1032115219832082No Hit
GGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCTCTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCC8530.10309066540008967No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT8440.10200295615202307No Hit
GCCCTGGGATTTTCAGGTGTTTTCATTTGGTGATCAGGACTGAACAGAGA8380.10127781665331202No Hit
GGCCAGGCCCCTGTGCTGGTCGTCTATGCTGATAGCGGCCGGCCCTCGGG8380.10127781665331202No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC8370.1011569600701935No Hit
CGGCCACCCTGACCATCAGCAGGGTCGAAGCCGGGGATGAGGCCGACTATTACTGTCAGGTGTGGGATAGTGTTA8330.10067353373771946No Hit
GTCCAGCACCCCAACGGCAACAAAGAAAAGAACGTGCCTCTTCCAGTGAT8320.10055267715460095No Hit
GGGTTGGGGCGGATGCACTCCCTGAGGCGACGGTGACCAGGGTTCCCTGGCCCCAGTTTTCAAAGTACCAACTG8310.10043182057148245No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGACG150.002391249668.73698430
TTATACC451.9465097E-645.700034
GTGGTAT2000.041.15491
GTATCAA19550.036.488571
TGGTATC2350.036.4738122
AATGGTA2600.035.1443670
CTTTGGA13800.035.0621569
GGATGGG8900.034.46742270
GTTATAC400.00267895534.2791673
ACAGACG5750.034.05291770
TAGGTAC552.6246923E-432.53762466
GCGTGTT13000.031.96403551
TATCAAC22350.031.9076462
GGAATTG13400.031.69987761
ATCAACG22450.031.607073
CTCGGCT12850.031.4819519
TCAACGC22650.031.3241944
ATTGTCT13200.031.24989764
CGGCTCT12950.031.2445121
TCGGCTC13100.031.1466220
Produced by FastQC (version 0.11.5)