FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689032

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689032
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1799334
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG83370.4633381017643195No Hit
GTACACAGGTTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA61600.3423488913120077No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41280.2294182180740207No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40540.22530558528877906No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA33740.18751382455953147No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33710.18734709620337303No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT33250.18479059474227685No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30780.17106329341856485No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29870.16600586661509203No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC29300.16283802784808157No Hit
CCTGTGTACCTTGCATGCAATAATAAACCCCGACATCCTCAGCTTCCACCCTGCTGATTTTCAGTGTGAAAT29010.16122632040521662No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT26270.1459984638760786No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25390.14110776542876421No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT24530.13632821921888877No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24280.13493881625090173No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT23310.129547932735112No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22740.12638009396810154No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT22690.1261022133745041No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22030.12243418953901833No Hit
ATATTGTACTGACCCAGACTCCACTCTCGTCACCTGTCACCCTTGGACAG21780.12104478657103128No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21660.1203778731463975No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21260.11815482839761823No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20620.1145979567995714No Hit
GAAATACACAGCCGTGTCTTCGGCTCCCAGGCTGTTCATTTGCAGATACA19810.11009629118329338No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCACCTCTAGTCAAAGCCTCGTA19670.10931822552122063No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18640.10359388529311402No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18610.10342715693695556No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA18400.10226005844384645No Hit
GTGTATTTCTGTGCGAGAGGCTTCGGGACCTTTGATAACTGGGGCCAGGG18250.10142641666305423No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26850.039.364521
CTAACGC957.2759576E-1238.8255843
TCGATAG350.001527407938.4388826
GTATCAA63450.035.536641
GTGGTAT8400.033.953021
AATGGTA7050.031.12101770
ATCAACG74950.029.974513
TATCAAC75200.029.8385642
TCAACGC75450.029.7717344
TGGTATC9500.029.3038142
CAACGCA77000.029.261145
AACGCAG77400.029.0666036
CGCAGAG80300.027.9333678
AGAGTAC81450.027.37355411
CCTAACG1354.2564352E-1027.3293042
CAGAGTA82150.027.22192810
GCAGAGT83000.027.0239419
GTACGAC500.00888916326.8249513
TCGTTAA1507.9333535E-526.59432470
CTGTCTT37250.026.05886770
Produced by FastQC (version 0.11.5)