FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689035

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689035
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1059189
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCAGCCTGCTCACAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATAGTGAGAGTGAAATCTGTC31730.29956882105082283No Hit
CAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATAGTGAGAGTGAAATCTGTCCCAGATCCACTGCCG21450.20251343244690043No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT20400.19260018750194724No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18000.16994134191348287No Hit
GATTAGGACCTTCGGGGCTTTCCCTGGTTTTTGCTGATACCACACTAACC16090.15190867729932997No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT15920.15030367573681372No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC14920.14086249007495358No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGTATTGGCAAGTGGTTAGTGTGGTATCAGCAAAAA14870.14039043079186056No Hit
ATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGTCCACCT14770.13944631222567455No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14650.13831336994625132No Hit
GTAATACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA14020.13236542297927942No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12960.12235776617770767No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG12670.1196198223357682No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12610.1190533511960566No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC12310.11622099549749856No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12230.11546570064454975No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT12120.11442717022174514No Hit
GGGTATTGGCAAGTGGTTAGTGTGGTATCAGCAAAAACCAGGGAAAGCCCCGAAGGTCCTAATCTACGCTGCATC11930.1126333449459917No Hit
GTATCAACGCAGAGTACGGGAAAGTGTCAGCCTGCTCACAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTG11730.11074510781361967No Hit
GTCCTAATCTACGCTGCATCCGTTTTGCAAAGTGGGGTCCCATCCAGGTT11690.11036746038714526No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11670.11017863667390805No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11630.10980098924743364No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG11550.10904569439448483No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11220.10593010312607098No Hit
GTGTGGTATCAGCAAAAACCAGGGAAAGCCCCGAAGGTCCTAATCTACGCTGCATCCGTTTTGCAAAGTGGGGTC11160.10536363198635937No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10930.10319215928413154No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT10740.1013983340083781No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGATC4350.038.9986227
TCGTATG2450.038.08712440
CACGAGA4450.038.0178321
ACGAGAC4500.037.5989622
GACCGAT4600.036.8512126
AGACCGA4750.036.3788625
CCGATCA4700.036.13382728
GTATCAA45050.036.0430261
TATCTCG3100.035.4225136
CCCACGA4750.034.8944819
GAGACCG4950.034.88590624
CGATCAG4900.034.68199529
CGTATGC2500.034.6273541
ATCTCGT3100.034.3599937
ATCTCCG4850.034.17500710
CTAACGC502.0899635E-433.8262833
CTTATAC5200.033.1773451
ATGCCGT2950.033.03748744
AGTATCG1155.2750693E-1132.71545834
GCCCACG5350.032.24561318
Produced by FastQC (version 0.11.5)