FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689036

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689036
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1059189
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42750.4036106870445218No Hit
GTGTCAGCCTGCTCACAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATAGTGAGAGTGAAATCTGTC33680.31797913309145015No Hit
CAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATAGTGAGAGTGAAATCTGTCCCAGATCCACTGCCG20890.1972263684762587No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT16750.15813985983615766No Hit
GATTAGGACCTTCGGGGCTTTCCCTGGTTTTTGCTGATACCACACTAACC15450.14586631847573944No Hit
GTCCTAATCTACGCTGCATCCGTTTTGCAAAGTGGGGTCCCATCCAGGTT15400.14539425919264645No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT15030.14190102049775818No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13500.12745600643511215No Hit
GTAATACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA13260.12519012187626571No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC12720.12009188161886122No Hit
ATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGTCCACCT12700.11990305790562403No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGTATTGGCAAGTGGTTAGTGTGGTATCAGCAAAAA12690.11980864604900542No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT12250.11565452435778693No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12070.11395511093865213No Hit
GTATCAACGCAGAGTACGGGAAAGTGTCAGCCTGCTCACAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTG11680.11027304853052666No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11510.10866804696801043No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG11400.10762951654520582No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11300.1066853979790198No Hit
GTGTGGTATCAGCAAAAACCAGGGAAAGCCCCGAAGGTCCTAATCTACGCTGCATCCGTTTTGCAAAGTGGGGTC11040.10423068970693615No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10700.10102068658190369No Hit
GGGTATTGGCAAGTGGTTAGTGTGGTATCAGCAAAAACCAGGGAAAGCCCCGAAGGTCCTAATCTACGCTGCATC10610.10017097987233628No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT807.8551886E-539.94305870
GTATCAA48500.036.5772631
AATGCCG800.003778143831.95444570
TATCAAC56350.031.4683782
ATCAACG56750.031.1782233
TCAACGC57600.030.8910774
TACACCG553.6972013E-430.6924615
AACGCAG58450.030.6136956
CAACGCA58400.030.5242845
CGCAGAG61400.029.2528178
ACGCAGA62100.028.8687067
CAGAGTA63650.028.05828910
AGAGTAC63350.027.97799511
GCAGAGT63850.027.97049
TATGTAT7300.027.13939370
CGAGATA500.0085757327.02212319
CCTAACG500.00857770527.020852
CTAACGC500.00858956927.0131913
GGTATCA32600.025.91291
CGCCCGA8700.024.97588770
Produced by FastQC (version 0.11.5)