FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004689037

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689037
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1657883
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAATATGATAAGTCACCGGAAACTTTTGGCCAGGGGA103040.6215155110463163No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT73970.44617141257857157No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC68520.4132981639838276No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGACTGTCCCAGACCCACTGCCA66570.40153617595451546No Hit
GTCTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAATATGATAAGTCA59890.36124382721820536No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA53410.3221578362284914No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC51690.3117831596077648No Hit
GTGTATTACTGTCAGCAATATGATAAGTCACCGGAAACTTTTGGCCAGGGGACCAAGCTGGAGGTCAAACGAA48840.2945925617187703No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG48640.2933862039721742No Hit
GAGTGAAGACTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGACGCCA45920.27697973861846703No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGACGCCAGTGGCCCTGCT45010.27149081087145477No Hit
GTCTGGGACAGTCTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT44280.2670876050963789No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC41920.25285258368654484No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT40880.24657952340424505No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC40620.2450112583336701No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG39020.23536039636090123No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA38810.2340937207269753No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGACTGTCCCAGACCCACTGCCA37470.2260111238247814No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT37340.2252269912894939No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG36330.21913488466918352No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG36250.2186523415705451No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG35910.21660153340133168No Hit
GTCTTTGTCTCCTGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA33440.20170301523086973No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT33000.1990490281883583No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCTGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGACAGTTGGCGG32000.19301723945537774No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT31330.18897594100428075No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT30060.18131556931339546No Hit
CTCCCAGACTCCTCATGTATGCTGCATCCAGCAGGGCCACTGGCGTCCCA28930.1744996480451274No Hit
CTCCAGGCACCCTGTCTTTGTCTCCTGGGGAAAGAGCCACCCTCTCCTGC27630.16665832269225272No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACCTCCAGCTTGGTCC27550.16617577959361426No Hit
CTCCTGCAGGGCCAGTCAGACAGTTGGCGGCAACTACTTAGCCTGGTACC27250.1643662429737201No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA26500.1598424014239847No Hit
GTGCAGCCACAGTTCGTTTGACCTCCAGCTTGGTCCCCTGGCCAAAAGTT26270.15845509001539915No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25920.15634396395885597No Hit
GTCAGACAGTTGGCGGCAACTACTTAGCCTGGTACCAGCAAAAACCTGGCCAGGCTCCCAGACTCCTCATGTATG25080.1512772614231523No Hit
CTACTTAGCCTGGTACCAGCAAAAACCTGGCCAGGCTCCCAGACTCCTCATGTATGCTGCATCCAGCAGGGCCA24630.14856295649331105No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT24510.14783914184535338No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24140.14560738001415058No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT24030.1449438832535227No Hit
GTCTGGGAGCCTGGCCAGGTTTTTGCTGGTACCAGGCTAAGTAGTTGCCG23840.1437978433942564No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC23640.1425914856476603No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA23400.14114385635174495No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG23280.1404200417037873No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22810.13758510099928645No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC22430.13529302128075382No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT21900.13209617325227413No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA21200.12787392113918775No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTC21140.1275120138152089No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG21010.12672788127992143No Hit
CCTCATGTATGCTGCATCCAGCAGGGCCACTGGCGTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGTCTT21000.12666756339259164No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT20970.12648660973060222No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC20250.12214372184285623No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA19930.12021354944830244No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT19900.12003259578631303No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA19770.11924846325102555No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG19750.11912782747636594No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT19580.11810242339175925No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19560.11798178761709963No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT19520.11774051606778042No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC19450.11731829085647179No Hit
CATGTATGCTGCATCCAGCAGGGCCACTGGCGTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGTCTTCA19330.1165944762085141No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC19050.11490557536327957No Hit
CTCCTCATGTATGCTGCATCCAGCAGGGCCACTGGCGTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGT18930.11418176071532188No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT18740.11303572085605558No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC18360.11074364113752297No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC17710.10682297846108561No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA17690.10670234268642599No Hit
GCCTGGTACCAGCAAAAACCTGGCCAGGCTCCCAGACTCCTCATGTATGCTGCATCCAGCAGGGCCACTGGCGTC17610.10621979958778756No Hit
CATGAGGAGTCTGGGAGCCTGGCCAGGTTTTTGCTGGTACCAGGCTAAGTAGTTGCCGCCAACTGTCTGACTGGC17510.1056166207144895No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC17410.10501344184119143No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT17360.10471185240454241No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT17230.10392771986925495No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC17090.10308326944663768No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGGTCAAACGAACTGTGGCTGCACCA16980.10241977268600981No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA16930.10211818324936078No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC16930.10211818324936078No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG16890.10187691170004155No Hit
CCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCCTGGGGAAAGAGCCAC16850.10163564015072235No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)