FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689038

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689038
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1657883
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAATATGATAAGTCACCGGAAACTTTTGGCCAGGGGA102720.6195853386517626No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT74780.45105716145228586No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGACTGTCCCAGACCCACTGCCA62640.3778312462339019No Hit
GTCTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAATATGATAAGTCA61810.372824861585528No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60950.3676375232751648No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC57360.34598340172376457No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC51230.30900853679059376No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA50660.3055704172127949No Hit
GTGTATTACTGTCAGCAATATGATAAGTCACCGGAAACTTTTGGCCAGGGGACCAAGCTGGAGGTCAAACGAA48950.2952560584793981No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG43590.26292567087062235No Hit
GTCTGGGACAGTCTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT42750.25785896833491867No Hit
GAGTGAAGACTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGACGCCA42090.2538779877711515No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC41580.25080177551733146No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT41110.24796683481283058No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC40990.24724302016487293No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGACGCCAGTGGCCCTGCT39740.23970328424864723No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGACTGTCCCAGACCCACTGCCA37300.22498571974017464No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA36610.22082378551441809No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG36190.21829043424656625No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT35620.21485231466876736No Hit
GTCTTTGTCTCCTGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA35420.21364595692217123No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT34850.21020783734437232No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG33380.20134110790689091No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG33330.20103951847024187No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCTGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGACAGTTGGCGG31310.18885530522962116No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT30900.18638227184909914No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT30730.18535686776449242No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA28670.1729313829745525No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28520.1720266146646054No Hit
CTCCAGGCACCCTGTCTTTGTCTCCTGGGGAAAGAGCCACCCTCTCCTGC27950.16858849508680648No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC27660.16683927635424212No Hit
CTCCCAGACTCCTCATGTATGCTGCATCCAGCAGGGCCACTGGCGTCCCA26950.16255670635382594No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT26150.1577312753674415No Hit
GTGCAGCCACAGTTCGTTTGACCTCCAGCTTGGTCCCCTGGCCAAAAGTT25990.1567661891701646No Hit
CTCCTGCAGGGCCAGTCAGACAGTTGGCGGCAACTACTTAGCCTGGTACC24090.14530579057750156No Hit
GTCTGGGAGCCTGGCCAGGTTTTTGCTGGTACCAGGCTAAGTAGTTGCCG23720.14307402874629874No Hit
GTCAGACAGTTGGCGGCAACTACTTAGCCTGGTACCAGCAAAAACCTGGCCAGGCTCCCAGACTCCTCATGTATG23630.14253116776033048No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC22860.13788669043593546No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT22800.13752478311195665No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT22760.1372835115626374No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACCTCCAGCTTGGTCC22620.13643906114002013No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG22450.13541365705541344No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22090.13324221311154044No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTC22070.13312157733688082No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC22040.1329406236748914No Hit
CTACTTAGCCTGGTACCAGCAAAAACCTGGCCAGGCTCCCAGACTCCTCATGTATGCTGCATCCAGCAGGGCCA21860.1318549017029549No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG21500.1296834577590819No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA21110.1273310601532195No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA20500.12365166902610135No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA20490.12359135113877155No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20430.12322944381479271No Hit
CCTCATGTATGCTGCATCCAGCAGGGCCACTGGCGTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGTCTT20090.12117863564557933No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19950.12033418522296206No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT19940.12027386733563226No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT19360.11677542987050353No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC19050.11490557536327957No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA18930.11418176071532188No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA18930.11418176071532188No Hit
CTCCTCATGTATGCTGCATCCAGCAGGGCCACTGGCGTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGT18830.11357858184202384No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA18360.11074364113752297No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT18280.11026109803888454No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC18070.10899442240495862No Hit
CATGTATGCTGCATCCAGCAGGGCCACTGGCGTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGTCTTCA17990.10851187930632017No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC17920.10808965409501152No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC17310.10441026296789338No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT17300.1043499450805636No Hit
GCCTGGTACCAGCAAAAACCTGGCCAGGCTCCCAGACTCCTCATGTATGCTGCATCCAGCAGGGCCACTGGCGTC17160.10350549465794631No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC17100.10314358733396747No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC17080.10302295155930785No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC16900.10193722958737136No Hit
GCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTCCAGGCACC16710.10079118972810507No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTGTG37850.044.52902670
GGTATCA14000.041.252421
GTATCAA31950.036.152351
ATCGGTG400.002845162333.8628428
GTATAAC400.002882075333.7739071
TACGTAT850.003689826532.11095470
GAACTGT34800.030.43901369
AATGGTA6800.030.1040270
ATACCGG450.00514983229.9977056
ATCAACG38850.029.3624633
TATCAAC38950.029.1190512
TCAACGC39800.028.6581424
CAACGCA40200.028.4586475
TGTTAGA20450.028.36205970
AACGCAG40550.028.213016
CCTAACG606.1613595E-428.1296372
CGAACTG34300.028.02811668
ACGAACT33400.027.41000467
CGCAGAG42850.026.7774168
ACGCAGA42900.026.6675427
Produced by FastQC (version 0.11.5)