FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689039

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689039
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1464305
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG48310.32991760596323855No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC38470.26271849102475237No Hit
GTAATAATCGGCCTCGTCCCCACTCTGGAGTCCGGTGATGCCCAGGGTGG36160.24694308904224188No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT35660.24352849986853833No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTAGTCCCTCCGCCGAACATCCC34660.23669932152113118No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT33260.22713847183476119No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA32390.221197086672517No Hit
GAAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGTAGATACA30960.2114313616357248No Hit
CTCATGTACAGCCTCTGGATTCACCTTTGACAAGTATGTCATGACCTGGGTCCGCCAGGCTCCAGGGAAGGGA30830.21054356845056185No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG30530.20849481494633976No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT29490.2013924694650363No Hit
ACATGGGATAGCAGCCTGCATGTTGGGATGTTCGGCGGAGGGACTAAGCTGACCGTCCTAGGTCAGCCCAAGG29400.20077784341376967No Hit
GATTATTACTGCGCAACATGGGATAGCAGCCTGCATGTTGGGATGTTCGG26680.18220247830882227No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG26280.17947080696985943No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC25790.17612450957962994No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT25780.17605621779615585No Hit
GGACTGGAGTGGGTCTCAAGTTGTGTCACTGGTGGTGACATTTATTATGA24760.16909045588180058No Hit
GACCTAGGACGGTCAGCTTAGTCCCTCCGCCGAACATCCCAACATGCAGG24080.16444661460556373No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTAGTCCCTC24030.16410515568819337No Hit
GCTTAGTCCCTCCGCCGAACATCCCAACATGCAGGCTGCTATCCCATGTTGCGCAGTAATAATCGGCCTCGTCCC23490.16041739938059352No Hit
GGATAGCAGCCTGCATGTTGGGATGTTCGGCGGAGGGACTAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC23130.15795889517552694No Hit
ATCCCATGTTGCGCAGTAATAATCGGCCTCGTCCCCACTCTGGAGTCCGG23060.15748085269120846No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC23050.1574125609077344No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA22900.1563881841556233No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT22310.1523589689306531No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC22210.1516760510959124No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21290.14539320701629782No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC21140.14436883026418676No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT20950.14307128637817942No Hit
GGACTAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG20940.14300299459470533No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT20850.1423883685434387No Hit
GTATATTTCTGTGCGAGAGAAGGAGAATCCCGTATCTTCGACTTTGACTT20740.1416371589252239No Hit
CTCCAGTCCCTTCCCTGGAGCCTGGCGGACCCAGGTCATGACATACTTGT20540.14027132325574249No Hit
CCCCCAAACTCCTCATTTATGACACTAATAAGCGAACCTCAGGGATTCCT20220.1380859861845722No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT19890.13583235732992785No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT19870.1356957737629797No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG19660.1342616463100242No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC19510.13323726955791315No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG19410.13255435172317243No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT19340.13207630923885394No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACAC19260.13152997497106134No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC19230.13132509962063915No Hit
GTCCAGCACCCCAACGGCAACAAAGAAAAGAACGTGCCTCTTCCAGTGAT19090.13036901465200215No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG19060.1301641393015799No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA19030.12995926395115773No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18290.12490567197407643No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG18080.12347154452112094No Hit
GTACCGGCCTGGGGCCTGGGGCTCAGGCATTGGGGCGCTGGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCT18000.12292521025332837No Hit
CCCTCAGTGTCTGCGGCCCCAGGACAGACGGTCACCATCTCCTGCTCTGGAAGCAGCTCCAACATCGGGAAAAAT17780.12142279101689879No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC17650.12053499783173588No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGACGGTCACCATCTCCTG17500.11951062107962482No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC17390.11875941146141003No Hit
CTGCTATCCCATGTTGCGCAGTAATAATCGGCCTCGTCCCCACTCTGGAG17390.11875941146141003No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC17380.11869111967793594No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17250.11780332649277302No Hit
GGGTTCCCTGGCCCCAGAAGTCAAAGTCGAAGATACGGGATTCTCCTTCTCTCGCACAGAAATATACGGCCGTGT16920.11554969763812865No Hit
GACCTGCCCTCGCCACTGAAGCAGACCATCTCCCGGCCCAAGGGGGTGGCCCTGCACAGGCCCGATGTCTACTTG16840.1150033633703361No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTCGTGGCTATTTTAAAAGGTGTCCAGTGTGAGGTGCAACTGCTGGAGT16770.1145253208860176No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16700.1140472784016991No Hit
GTGCTGGACTTTGCACACCACGTGTTCGTCTGTGCCCTGCATGACGTCCT16590.11329606878348432No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG16450.1123399838148473No Hit
CCTGTGAGGTGGCTGCGTACTTGCCCCCTCTCAGGACTGATGGGAAGCCC16420.1121351084644251No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG16210.11070098101146959No Hit
CCTCAGGGATTCCTGACCGATTCTCTGGCTCCAAGTCTGGCACGACAGCC16080.10981318782630667No Hit
GGGTGGCTGTCGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGAATCCCT15990.10919856177504003No Hit
AGGCAGGACTCGGGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGGT15950.10892539464114376No Hit
GGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCTCTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCC15790.10783272610555861No Hit
GGTCTGTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGCCTCACCCA15740.10749126718818824No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT15660.10694493292039567No Hit
GTTTGGGGGTTGTTCCTGGGCGTTGGTGGTACCACGATACAGGATTTTTC15470.10564738903438833No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC15450.10551080546744018No Hit
CCCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGAAGTCAAAGTCGA15320.10462301228227726No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTCGT15320.10462301228227726No Hit
GCCCAAGGGGGTGGCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAG14870.10154988202594406No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14760.10079867240772927No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG14720.10052550527383299No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG4600.081.4578770
TATCGTA150.00237499168.8580138
ATATCGT252.6113912E-455.01051737
GTACTAA200.007709021751.1460721
AGGTACG306.692379E-445.4569633
CGTATGC6950.044.7525641
TCGTATG6950.044.70922540
GACGCGT7150.044.3777226
ATGCCGT6900.044.23901744
ACGCGTA7100.044.2263327
TCTCGTA6400.044.11216438
GCCGTCT6900.043.87503446
AGACGCG7250.043.7581425
ACGAGAC7350.043.60157422
GGTATCA10200.043.4574431
CGAGACG7300.043.4361623
GTGGTAT2200.043.3966641
CCTAACG551.6139347E-743.3907362
CTAACGC551.6139347E-743.3907363
AATCTCG6450.043.1263636
Produced by FastQC (version 0.11.5)