FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689040

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689040
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1464305
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG63390.43290161544213807No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG50920.34774176144997115No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC36670.2504259699994195No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA34620.23642615438723488No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGACGGTCACCATCTCCTG33830.23103110349278325No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT32920.2248165511966428No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG30960.2114313616357248No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC30100.20555826825695467No Hit
GAAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGTAGATACA29670.20262172156756958No Hit
GTAATAATCGGCCTCGTCCCCACTCTGGAGTCCGGTGATGCCCAGGGTGG29430.2009827187641919No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTAGTCCCTCCGCCGAACATCCC29300.20009492557902897No Hit
CTCATGTACAGCCTCTGGATTCACCTTTGACAAGTATGTCATGACCTGGGTCCGCCAGGCTCCAGGGAAGGGA29120.1988656734764957No Hit
ACATGGGATAGCAGCCTGCATGTTGGGATGTTCGGCGGAGGGACTAAGCTGACCGTCCTAGGTCAGCCCAAGG26820.18315856327745927No Hit
GATTATTACTGCGCAACATGGGATAGCAGCCTGCATGTTGGGATGTTCGG24470.16710999416105252No Hit
GGACTGGAGTGGGTCTCAAGTTGTGTCACTGGTGGTGACATTTATTATGA24210.16533440779072664No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT23740.1621246939674453No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG23560.16089544186491203No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23480.16034910759711946No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC23320.1592564390615343No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT22830.15591014167130482No Hit
GTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGACGGTCAC22460.15338334568276418No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA22050.15058338256032727No Hit
GGATAGCAGCCTGCATGTTGGGATGTTCGGCGGAGGGACTAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC21700.14819317013873476No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT21490.14675904268577925No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACAC21360.14587124950061633No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC21030.14361762064597197No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC20940.14300299459470533No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT20920.14286641102775718No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG20870.14252495211038685No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT20690.14129570000785355No Hit
GGACTAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG20640.1409542410904832No Hit
GCTTAGTCCCTCCGCCGAACATCCCAACATGCAGGCTGCTATCCCATGTTGCGCAGTAATAATCGGCCTCGTCCC20190.13788111083414997No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTAGTCCCTC20060.13699331764898706No Hit
CTCCAGTCCCTTCCCTGGAGCCTGGCGGACCCAGGTCATGACATACTTGT20020.1367201505150908No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC19570.13364702025875755No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC19410.13255435172317243No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19340.13207630923885394No Hit
GTATATTTCTGTGCGAGAGAAGGAGAATCCCGTATCTTCGACTTTGACTT19080.13030072286852806No Hit
GACCTAGGACGGTCAGCTTAGTCCCTCCGCCGAACATCCCAACATGCAGG19080.13030072286852806No Hit
GTCCAGCACCCCAACGGCAACAAAGAAAAGAACGTGCCTCTTCCAGTGAT18800.1283885529312541No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT18650.127364176179143No Hit
GGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCTCTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCC18540.12661296656092821No Hit
CCCCCAAACTCCTCATTTATGACACTAATAAGCGAACCTCAGGGATTCCT18500.12633979942703194No Hit
ATCCCATGTTGCGCAGTAATAATCGGCCTCGTCCCCACTCTGGAGTCCGG18490.12627150764355788No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG18240.12456421305670608No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT18120.12374471165501723No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17470.11930574572920258No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC17270.11793991005972115No Hit
GGGTTCCCTGGCCCCAGAAGTCAAAGTCGAAGATACGGGATTCTCCTTCTCTCGCACAGAAATATACGGCCGTGT17100.11677894974066196No Hit
GTACCGGCCTGGGGCCTGGGGCTCAGGCATTGGGGCGCTGGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCT17030.11630090725634344No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16830.11493507158686202No Hit
GGGTGGCTGTCGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGAATCCCT16660.11377411126780282No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC16490.1126131509487436No Hit
GTGCTGGACTTTGCACACCACGTGTTCGTCTGTGCCCTGCATGACGTCCT16270.11111073171231403No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16080.10981318782630667No Hit
GGTCTGTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGCCTCACCCA15920.10872051929072153No Hit
CCCTCAGTGTCTGCGGCCCCAGGACAGACGGTCACCATCTCCTGCTCTGGAAGCAGCTCCAACATCGGGAAAAAT15900.10858393572377338No Hit
CCCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGAAGTCAAAGTCGA15770.10769614253861046No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG15640.10680834935344755No Hit
GCCCTGGGATTTTCCGGTGTTTTCATTTGGTGATCAGGACTGAACAGAGA15590.10646689043607718No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTCGTGGCTATTTTAAAAGGTGTCCAGTGTGAGGTGCAACTGCTGGAGT15580.10639859865260312No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG15530.10605713973523276No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG15370.10496447119964761No Hit
GTATCAACGCAGAGTACGGGAAGCTCTGCTTCAGCTGTGAGCGCAGAAGG15360.10489617941617355No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT15290.10441813693185505No Hit
CCTCAGGGATTCCTGACCGATTCTCTGGCTCCAAGTCTGGCACGACAGCC14980.10230109164415883No Hit
GTCCAGGAGATGGTCACGCTGTCATAGGTGGTCAGGTCTGTGACCAGGCA14920.10189134094331441No Hit
AGGCAGGACTCGGGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGGT14900.10175475737636626No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG14900.10175475737636626No Hit
CTGCTATCCCATGTTGCGCAGTAATAATCGGCCTCGTCCCCACTCTGGAG14890.1016864655928922No Hit
GGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTG14840.10134500667552182No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTAGTCCCTCCGCCGAACAT14820.10120842310857368No Hit
GGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCTGAACCTGCGG14780.10093525597467741No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAATG100.009402814131.9349870
CGTTTAA601.5438673E-765.9674970
TACGTAT651.9121606E-550.7442370
CCGTTTA551.7827624E-646.28937569
GGTATCA13050.043.3587041
GTGGTAT3200.037.281791
GGATGGG18850.035.69593470
TCGCTCG750.002357929235.18266370
GTATCAA28800.035.033051
GACAGGT2500.034.086211
ACGGGTA1155.4569682E-1132.5808216
CACCGTT603.6829864E-430.71340667
ATCAACG32450.030.5485043
TCAACGC32800.030.3263854
TATCAAC32900.030.2414382
TGGTATC4000.029.8142262
CAACGCA33850.029.2820475
AACGCAG34150.029.02586
TTCACTA500.006388416528.70314262
AGGATGG15950.028.46530769
Produced by FastQC (version 0.11.5)