FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689041

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689041
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences552231
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC11570.20951377231629517No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC10630.1924919100883507No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC9870.1787295533934169No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT8680.1571806001474021No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC7670.13889115243439792No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT7340.13291539229054508No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT6520.11806653375127436No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT6460.1169800319069375No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC6420.11625569734404625No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC6300.11408269365537248No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT6170.11172860632597591No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC5870.10629609710429151No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC5870.10629609710429151No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATA200.007979196550.69811219
TCGTATG451.870796E-645.95979740
GCCGTCT503.5179637E-641.92917346
GTATCAA21100.036.3814471
GGTATCA11050.036.1123921
TCTCGCG2650.031.33104370
ATCAACG25400.030.3445243
CTCGTAT802.8253035E-630.09441439
TATCAAC25400.030.0783442
TCAACGC25700.029.9903094
CGTATGC703.819375E-529.60299541
CAACGCA26400.029.4512085
AACGCAG26850.029.0835136
TATAGTG1401.8189894E-1128.9755975
CCGTCTT853.9509177E-628.82619347
TCTCGTA854.59874E-628.26944738
ATCTCGT854.6549976E-628.22531737
ATGCCGT755.818612E-527.8303344
CGCAGAG28400.027.7267388
GTAGTTT2553.8380676E-1027.55052870
Produced by FastQC (version 0.11.5)