FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689044

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689044
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences787701
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27300.3465782067053362No Hit
GTAATAAACGGCCGTGTCAGCGGCGATCACAGAGGTCAGTATCAGGGAGA26350.3345177929188867No Hit
CCCTGATACTGACCTCTGTGATCGCCGCTGACACGGCCGTTTATTACTGTGCGAGGCACTACGACAGTAGTTTC22420.2846257653602065No Hit
TCCTACTACTACCCCACCTTCAAGAGTCGAGCCACCATATCAGTAGACACGTCCAGGAACCAGTTCTCCCTGATA22200.2818328274307129No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA20820.26431348950934425No Hit
GTCTCTGCTTACTCCATCAGCAGTGGTTTCTACTGGGCCTGGATCCGCCA19780.2511105102062839No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19650.24946013779340132No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18580.2358763033181372No Hit
GTTTATTACTGTGCGAGGCACTACGACAGTAGTTTCCTGGACTATTGGAGCCAGGGAACCCTGGTCACCGTCTC18430.23397202745711887No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17560.22292722746321256No Hit
CACCTGCACTGTCTCTGCTTACTCCATCAGCAGTGGTTTCTACTGGGCCT17520.222419420566941No Hit
GTATCAGGGAGAACTGGTTCCTGGACGTGTCTACTGATATGGTGGCTCGA17160.21784915850049702No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGTGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGTAA16890.21442146195066403No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16410.20832777919540538No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16000.2031227585086219No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15890.20172628954387514No Hit
GTCTGGGACAGACTTCACTCTCTCCATCAGCCGCCTAGAGGCTGAAGATT15650.19867944816624583No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15020.19068148954996883No Hit
GTCATGGACCTCCTGTGCAAGAACATGAAGCACCTGTGGTTTTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC14940.18966587575742572No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14570.18496866196691383No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14530.1844608550706423No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14430.18319133782996339No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14320.18179486886521662No Hit
GTCTTTGTCTCCAGGGGAAAGTGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGTAACTACCTAGCCTGGTA13680.17366995852487174No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGTAACTACCTAGCCTGGTACCAACAGAAGCCTGGCCAGGCTCCCAGGCT13550.1720195861119892No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13400.17011531025097085No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13070.16592590335673055No Hit
GACTTCACTCTCTCCATCAGCCGCCTAGAGGCTGAAGATTTTGCAGTTTATTACTGTCAGCACCGTAGCAGCTGG12850.163132965427237No Hit
GTTTATTACTGTCAGCACCGTAGCAGCTGGCCGATCACCTTCGGCCGAGGGACACGACTGGAGATTAAACGAA12550.1593244137052003No Hit
GTAGTAGGATGTCCCACCATGATGGATGCTCCCAATCCACTCCAGCCCCCTCCCTGGGGACTGGCGGATCCAGGC12530.15907051025706456No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA12360.15691233094791043No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC11850.1504377930204481No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11840.15031084129638023No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11720.14878742060756556No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT11610.1473909516428188No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11410.144851917161461No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC11300.14345544819671424No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGTCA11250.14282068957637478No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11170.14180507578383167No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGTCAGTGGCCCTGTT11150.14155117233569592No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGGCTCCAATAGTCCAGGAAACTA11040.14015470337094912No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTCGGCCGAAGGTG10980.1393929930265418No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10910.13850433095806658No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10760.13660005509704823No Hit
GGGTTCCCTGGCTCCAATAGTCCAGGAAACTACTGTCGTAGTGCCTCGCACAGTAATAAACGGCCGTGTCAGCGG10630.13494968268416568No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10590.13444187578789416No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10580.13431492406382625No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTCGGCCGAAGGTGATCGGCCAG10560.13406102061569047No Hit
ATACTGACCTCTGTGATCGCCGCTGACACGGCCGTTTATTACTGTGCGAGGCACTACGACAGTAGTTTCCTGGA10550.13393406889162257No Hit
GGTTTCTACTGGGCCTGGATCCGCCAGTCCCCAGGGAGGGGGCTGGAGTG10540.1338071171675547No Hit
GTAATAAACTGCAAAATCTTCAGCCTCTAGGCGGCTGATGGAGAGAGTGAAGTCTGTCCCAGACCCACTGCCA10480.13304540682314736No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10460.13279150337501158No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC10390.13190284130653637No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG10350.1313950344102648No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10330.13114113096212904No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG10170.1291099033770428No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG9770.12403183441432727No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT9760.12390488269025937No Hit
CTCCATCAGCCGCCTAGAGGCTGAAGATTTTGCAGTTTATTACTGTCAGCACCGTAGCAGCTGGCCGATCACCTT9570.12149279993296949No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9560.12136584820890162No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT9510.12073108958856216No Hit
GTCCCTCACCTGCACTGTCTCTGCTTACTCCATCAGCAGTGGTTTCTACT9370.11895376545161171No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9340.11857291027940806No Hit
GAGTGTTAGTAACTACCTAGCCTGGTACCAACAGAAGCCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATC9320.11831900683127226No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC9310.11819205510720439No Hit
GTGCAAGAACATGAAGCACCTGTGGTTTTTCCTCCTGCTGGTGGCAGCTC9130.11590692407398238No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT9120.11577997234991451No Hit
GTGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGTAACTACCTAGCCTGGTACCAACAGAAGCCTGGCCAGG9110.1156530206258466No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG9100.11552606890177872No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG8970.11387569648889616No Hit
CCGTAGCAGCTGGCCGATCACCTTCGGCCGAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT8940.11349484131669249No Hit
GTGTTAGTAACTACCTAGCCTGGTACCAACAGAAGCCTGGCCAGGCTCCC8940.11349484131669249No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACACAGTC8900.11298703442042095No Hit
GTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGAGTGGCTCCTGGGG8870.11260617924821728No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC8860.1124792275241494No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGTGC8810.11184446890380995No Hit
TCTATGATGCATCCAACAGGGCCACTGACATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT8700.11044799993906317No Hit
GGTCAGTATCAGGGAGAACTGGTTCCTGGACGTGTCTACTGATATGGTGG8580.10892457925024851No Hit
GGACTGGTGAAGCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGCTTACTCCATCAGCAGTGGTTTCTA8560.10867067580211272No Hit
GGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGACCTGTACACCACGAGCAGCCAGCTGAC8540.10841677235397695No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT8480.10765506200956963No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG8380.10638554476889073No Hit
GTCAGAGTGTTAGTAACTACCTAGCCTGGTACCAACAGAAGCCTGGCCAGGCTCCCAGGCTCCTCATCTATGATG8340.10587773787261918No Hit
GGATTGGGAGCATCCATCATGGTGGGACATCCTACTACTACCCCACCTTC8320.10562383442448339No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8270.10498907580414396No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7890.1001649102895642No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG7880.1000379585654963No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)