FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689045

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689045
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1700333
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTACAGTACCCCGCTCACTTTTGGCGGAGGGACCAGGGTGGAGATCAAACGAACTGTGGCTGCACCAT67190.39515788966043713No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT65130.38304261576996973No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC63660.37439725042094696No Hit
GTAATAGACGGCCGTGTCCGCAGCGGTCAGAGAGGTCAGACTCAGGGAGA52770.31035097242716575No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC46630.2742403987924718No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG45100.26524216138838685No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG39900.2346599166163334No Hit
CTGTAACCCTGTTGACAGTAGTAAGTTGCAAAGTCCTCAGGTTGCAGAGTGCTGATGGTGAGAGTGAAATCTGTC39400.23171931615748206No Hit
ACAGTACCCCGCTCACTTTTGGCGGAGGGACCAGGGTGGAGATCAAACGA39220.2306606999922956No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC38610.22707316743249706No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT38460.22619098729484166No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC37240.21901592217524452No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA35570.20919431664268118No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT34380.20219568755061507No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG34050.2002548912477732No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCAGCATCACTT33980.19984320718353407No Hit
GGGTACTGTAACCCTGTTGACAGTAGTAAGTTGCAAAGTCCTCAGGTTGCAGAGTGCTGATGGTGAGAGTGAAAT33950.19966677115600298No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG33690.1981376589174003No Hit
GTCTATTACTGTGCGAAAGATCGAATTCTGACTTCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCCCTGCATCC33520.19713785476139084No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG33060.19443250233924766No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG32340.1901980376785018No Hit
GTCTCTGGTGGCTCCATCAGTGGTTCTTATTGGAACTGGATCCGGCAGCC31670.18625763306364107No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG31610.18590476100857892No Hit
GTAGGAGACAGAGTCAGCATCACTTGCCGGACAAGTCAGACCATTATCAGGTATATAAACTGGTATCAGCAGATC31350.18437564876997622No Hit
GATCTATGCTGCATCAACTTTGCAAAGCGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCA31280.18396396470573706No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT31050.18261128849466546No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC30380.17867088387980473No Hit
GTATATAAACTGGTATCAGCAGATCCCAGGGAAAGCCCCTAAACTCCTGATCTATGCTGCATCAACTTTGCAAAG29960.17620077949436963No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT29820.17537741136589127No Hit
CTCCTGATCTATGCTGCATCAACTTTGCAAAGCGGGGTCCCATCAAGGTT28470.16743779012699278No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCCCTCCGCCAAAAGTGAGCGGGGTA27360.1609096571083429No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT27300.16055678505328075No Hit
CCTGAGGACTTTGCAACTTACTACTGTCAACAGGGTTACAGTACCCCGCT27090.1593217328605632No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA26220.15420508806216193No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT26200.15408746404380788No Hit
GTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACCT26180.15396984002545383No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCT25660.1509116155482485No Hit
GTCCTCAGGTTGCAGAGTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGAC25310.1488531952270526No Hit
ACTTACTACTGTCAACAGGGTTACAGTACCCCGCTCACTTTTGGCGGAGGGACCAGGGTGGAGATCAAACGAA24920.14655952686914858No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT24870.14626546682326344No Hit
GATCAGGAGTTTAGGGGCTTTCCCTGGGATCTGCTGATACCAGTTTATAT24680.14514803864889994No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA24680.14514803864889994No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23890.1405018899239149No Hit
CTTTTGGCGGAGGGACCAGGGTGGAGATCAAACGAACTGTGGCTGCACCA22700.13350326083184882No Hit
AGTCAGACCATTATCAGGTATATAAACTGGTATCAGCAGATCCCAGGGAAAGCCCCTAAACTCCTGATCTATG22620.13303276475843262No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT22550.13262108069419343No Hit
GATTTCACTCTCACCATCAGCACTCTGCAACCTGAGGACTTTGCAACTTACTACTGTCAACAGGGTTACAGTACC22460.1320917726116002No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22100.12997454028122726No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22040.1296216682261651No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG21740.12785730795085432No Hit
GTAGTAAGTTGCAAAGTCCTCAGGTTGCAGAGTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCG21620.12715156384073004No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC21610.127092751831553No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT21480.12632819571225168No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCCCTCCGCCAAAAGTG21480.12632819571225168No Hit
CCATCAGCACTCTGCAACCTGAGGACTTTGCAACTTACTACTGTCAACAG20600.12115273890467339No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG20530.12074105484043421No Hit
GCATAGATCAGGAGTTTAGGGGCTTTCCCTGGGATCTGCTGATACCAGTT20390.11991768671195584No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGTGGTTCTTATTGGAACTGGATCCGGCAGCCCCCAGGGAAGGGA20120.11832976246417613No Hit
GTCTGCATCTGTAGGAGACAGAGTCAGCATCACTTGCCGGACAAGTCAGA20070.11803570241829099No Hit
GTTTATATACCTGATAATGGTCTGACTTGTCCGGCAAGTGATGCTGACTC19960.11738877031734372No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19800.11644777817051132No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT19610.1153303499961478No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19490.11462460588602351No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG19130.11250737355565057No Hit
GGGTATATCTACTACAGTGGTACCACCAACTCCCAAACCAATTACTACCC19120.11244856154647355No Hit
ATGCTGCATCAACTTTGCAAAGCGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCA18850.11086063729869385No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC18810.11062538926198573No Hit
ATCTACTACAGTGGTACCACCAACTCCCAAACCAATTACTACCCCTCCCT18790.11050776524363169No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA18530.108978653005029No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG18510.10886102898667496No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18510.10886102898667496No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA18440.10844934492243577No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC18340.10786122483066553No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT18300.10762597679395741No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC18180.10692023268383312No Hit
GACCAGGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT18130.10662617263794798No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC18110.10650854861959393No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT17900.10527349642687639No Hit
GGTATCAGCAGATCCCAGGGAAAGCCCCTAAACTCCTGATCTATGCTGCATCAACTTTGCAAAGCGGGGTCCCAT17880.10515587240852234No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17880.10515587240852234No Hit
CCCCTAAACTCCTGATCTATGCTGCATCAACTTTGCAAAGCGGGGTCCCA17810.10474418834428316No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT17680.10397963222498181No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA17200.10115665578448456No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC17150.10086259573859944No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA352.3162396E-549.1301338
ATCGCGT652.237357E-1047.5563137
CGCGTAT405.05097E-543.0553439
CGTATGC1400.039.45522341
GATACCG350.001429073938.96283326
GTGGTAT3150.038.9433481
GTATCAA33600.037.1178781
TCGTATG1400.036.95072640
CGAGACT4500.036.34391823
ACGAGAC4600.036.292422
CACGAGA4750.035.14632421
CTTATAC4700.034.8004381
TCGCGTT400.002637248334.3910938
CATCGCG1300.034.3116336
CGGTGTA400.00270982934.201231
TATACAC5250.032.447063
ATCTCCG5150.032.4098410
TATCAAC39050.031.93192
ATCAACG39450.031.694493
ATGCCGT1650.031.5163544
Produced by FastQC (version 0.11.5)