FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689046

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689046
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1700333
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTACAGTACCCCGCTCACTTTTGGCGGAGGGACCAGGGTGGAGATCAAACGAACTGTGGCTGCACCAT73170.4303274711482986No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT65140.3831014277791468No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC51900.3052343276287645No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG49330.29011964127026885No Hit
GTAATAGACGGCCGTGTCCGCAGCGGTCAGAGAGGTCAGACTCAGGGAGA48420.28476774843515945No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC47700.2805332837744136No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG45540.267829889792176No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT39170.2303666399464105No Hit
CTGTAACCCTGTTGACAGTAGTAAGTTGCAAAGTCCTCAGGTTGCAGAGTGCTGATGGTGAGAGTGAAATCTGTC38880.22866109168027676No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC37670.2215448385698566No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36850.2167222538173405No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG36560.21501670555120672No Hit
GGGTACTGTAACCCTGTTGACAGTAGTAAGTTGCAAAGTCCTCAGGTTGCAGAGTGCTGATGGTGAGAGTGAAAT34630.20366598778004072No Hit
ACAGTACCCCGCTCACTTTTGGCGGAGGGACCAGGGTGGAGATCAAACGA33910.19943152311929488No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT33650.19790241088069221No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG32350.19025684968767884No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA32160.18913942151331534No Hit
GTCTATTACTGTGCGAAAGATCGAATTCTGACTTCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCCCTGCATCC32080.18866892543989913No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCAGCATCACTT31530.1854342649351627No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT31330.18425802475162217No Hit
GTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACCT31300.1840815887240911No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG30430.17896494392568985No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG30100.177024147622848No Hit
GATCTATGCTGCATCAACTTTGCAAAGCGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCA29800.17525978734753722No Hit
GTAGGAGACAGAGTCAGCATCACTTGCCGGACAAGTCAGACCATTATCAGGTATATAAACTGGTATCAGCAGATC29740.17490691529247507No Hit
GTCTCTGGTGGCTCCATCAGTGGTTCTTATTGGAACTGGATCCGGCAGCC29300.17231918688868592No Hit
CTCCTGATCTATGCTGCATCAACTTTGCAAAGCGGGGTCCCATCAAGGTT29190.17167225478773865No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG28210.1659086778883901No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT27840.16373263354884013No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC27790.16343857350295501No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT27760.16326213747542392No Hit
GTCCTCAGGTTGCAGAGTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGAC27630.16249758135612258No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT27140.15961579290644834No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCT26930.1583807407137308No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA26830.15779262062196053No Hit
GTATATAAACTGGTATCAGCAGATCCCAGGGAAAGCCCCTAAACTCCTGATCTATGCTGCATCAACTTTGCAAAG26760.15738093655772134No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCCCTCCGCCAAAAGTGAGCGGGGTA25680.15102923956660255No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25450.14967656335553095No Hit
ACTTACTACTGTCAACAGGGTTACAGTACCCCGCTCACTTTTGGCGGAGGGACCAGGGTGGAGATCAAACGAA24050.14144288207074732No Hit
CCTGAGGACTTTGCAACTTACTACTGTCAACAGGGTTACAGTACCCCGCT23910.14061951394226896No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT23440.13785534951094872No Hit
GTAGTAAGTTGCAAAGTCCTCAGGTTGCAGAGTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCG23230.1366202973182312No Hit
GATCAGGAGTTTAGGGGCTTTCCCTGGGATCTGCTGATACCAGTTTATAT23150.136149801244815No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22690.13344444882267179No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCCCTCCGCCAAAAGTG22650.1332092007859637No Hit
CTTTTGGCGGAGGGACCAGGGTGGAGATCAAACGAACTGTGGCTGCACCA22030.12956285621698807No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT21980.12926879617110296No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA21590.12697512781319895No Hit
AGTCAGACCATTATCAGGTATATAAACTGGTATCAGCAGATCCCAGGGAAAGCCCCTAAACTCCTGATCTATG21390.1257988876296584No Hit
GATTTCACTCTCACCATCAGCACTCTGCAACCTGAGGACTTTGCAACTTACTACTGTCAACAGGGTTACAGTACC21330.12544601557459628No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG21250.12497551950118008No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20920.1230347231983382No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT20860.12268185114327605No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC20740.12197610703315175No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20180.11868263451923829No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19910.11709471027145858No Hit
GCATAGATCAGGAGTTTAGGGGCTTTCCCTGGGATCTGCTGATACCAGTT19890.11697708625310455No Hit
GTTTATATACCTGATAATGGTCTGACTTGTCCGGCAAGTGATGCTGACTC19850.11674183821639643No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCG19670.11568322205120997No Hit
GGGTATATCTACTACAGTGGTACCACCAACTCCCAAACCAATTACTACCC19620.11538916200532483No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC19580.11515391396861674No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19520.11480104191355459No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT19400.11409529780343027No Hit
GGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG19390.11403648579425324No Hit
CCATCAGCACTCTGCAACCTGAGGACTTTGCAACTTACTACTGTCAACAG19190.11286024561071271No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA19030.1119192534638803No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18730.11015489318856952No Hit
GTCTGCATCTGTAGGAGACAGAGTCAGCATCACTTGCCGGACAAGTCAGA18530.108978653005029No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGTGGTTCTTATTGGAACTGGATCCGGCAGCCCCCAGGGAAGGGA18260.10739072875724932No Hit
ATGCTGCATCAACTTTGCAAAGCGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCA18080.10633211259206284No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA17980.1057439925002926No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17970.10568518049111557No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17910.1053323084360534No Hit
ATCTACTACAGTGGTACCACCAACTCCCAAACCAATTACTACCCCTCCCT17810.10474418834428316No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT17790.1046265643259291No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA17550.10321507610568047No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC17480.10280339204144129No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC17240.10139190382119267No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA17210.10121546779366161No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATCG350.005274879756.2936770
CCGTTCG200.00778577151.0184179
GTGGTAT3500.047.6564331
GTATCAA36100.037.8120881
TACGTAT700.00171765737.52911470
CCGTTAA808.027382E-834.02028316
TATCAAC41400.032.8775752
GCCGTTA851.3678255E-732.01720415
ATCAACG42300.031.7627413
TCAACGC43000.031.3229354
CCGTCAT850.004449392730.90633270
CAACGCA43800.030.7508265
AACGCAG44350.030.3694746
GGTACCG902.2614404E-730.2331376
TGTTAGA18150.030.0336370
ACGCAGA46400.029.101027
CCGTAGC500.006047735529.02722565
CGCAGAG47300.028.5473048
AGAGTAC47600.028.36738211
GATTCAC3000.028.3669221
Produced by FastQC (version 0.11.5)