FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689047

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689047
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1071533
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT51890.48425946751056664No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC47660.444783315119553No Hit
GTATATTACTGTGCGAGAGATCGGTACTGCAGCAGTACTACTTGCTATGATGGAGACTATTATTACTTTGACTA42480.3964413601820943No Hit
GATCTATGCTGCATCCAGATTGGAAAGTGGGGTCCCATCAAGGTTCAGAGGCAGTGGATCTGGGACAGATTTCA37170.3468861901593325No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC35990.3358739301542743No Hit
GTCCTGATCTATGCTGCATCCAGATTGGAAAGTGGGGTCCCATCAAGGTT34800.32476834591188514No Hit
GTAATATACAGCCGTGTCTTCAGATCTCAGGCTGCTCAGCTCCATGTAGG31090.29014505386208356No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30820.28762529945414655No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG30470.2843589511475615No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29960.2795994150436804No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28700.26784056113997423No Hit
GTCTCCATCCTCCCTGTCTGCATCTATAGGAGACAGAGTCACCATCACTT26790.25001563180975295No Hit
ATAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAACATTGGCAGTTATTTAAATTGGTATCAGCAGAAA26670.24889574096178094No Hit
GATCAGGACCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAT26520.24749587740181592No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25980.2424563685859418No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25850.24124315350063882No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25060.2338705387514897No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24860.23200405400486965No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24410.2278044633249746No Hit
AGTCAGAACATTGGCAGTTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGGTCCTGATCTATG23510.2194052819651845No Hit
GCATAGATCAGGACCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT23480.21912530925319146No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG23060.21520569128528938No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22600.21091277636806333No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22530.2102595067067463No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC22400.20904629162144328No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22340.2084863461974573No Hit
GTCTGGGGCTGAGGTGAAGAAGTCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACAGCTTCACTAG22130.20652653721350625No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21880.20419343128023124No Hit
GAGTCACAGTACCCCCTCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT21860.20400678280556922No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACCTCTGTCAACAGAGTCACAGTACC19470.18170229008345987No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19380.18086237194748087No Hit
CCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCA19350.18058239923548783No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT19220.17936918415018482No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18620.17376972991032474No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18490.17255651482502174No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC18390.17162327245171172No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17830.16639711516117564No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17790.1660238182118516No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT17600.16425065770256259No Hit
GTAATATACAGCCGTGTCTTCAGATCTCAGGCTGCTCAGCTCCATGTAGGCTGTGCTCGCGGATGTGTCGCTGGT16980.15846455498804052No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG16910.15781128532672348No Hit
CCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATAACTGCCA16900.15771796108939248No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16820.15697136719074448No Hit
GTTCAGAGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTA16810.15687804295341348No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16700.15585147634277247No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16470.1537050188841594No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16390.1529584249855114No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16120.15043867057757437No Hit
GCCTACATGGAGCTGAGCAGCCTGAGATCTGAAGACACGGCTGTATATTA15700.14651905260967232No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG15470.1443725951510593No Hit
CTGTGACTCTGTTGACAGAGGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC15350.14325270430308726No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC15280.14259943464177025No Hit
AGGGGGTACTGTGACTCTGTTGACAGAGGTAAGTTGCAAAATCTTCAGGT15060.1405463014204882No Hit
GTACCCCCTCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGA14910.1391464378605232No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14900.1390531136231922No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14780.13793322277522016No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCTCTGAACCTTGATGGGACCCCA14760.13774657430055817No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14690.13709330463924116No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14620.13644003497792415No Hit
CCCCTAAGGTCCTGATCTATGCTGCATCCAGATTGGAAAGTGGGGTCCCA14470.13504017141795913No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14350.13392028056998712No Hit
CAGTACCCCCTCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTT14290.1333603351460011No Hit
CTATTATTACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGT14110.1316804988740431No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC13890.12962736565276103No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13850.12925406870343703No Hit
CTATAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAACATTGGC13450.125521099210197No Hit
CCATCCTCCCTGTCTGCATCTATAGGAGACAGAGTCACCATCACTTGCCG13330.12440120836222496No Hit
CGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT13180.12300134480225994No Hit
GTAATAATAGTCTCCATCATAGCAAGTAGTACTGCTGCAGTACCGATCTCTCGCACAGTAATATACAGCCGTGT13170.12290802056492893No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA13170.12290802056492893No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13140.12262804785293593No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC13110.12234807514094294No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13100.12225475090361193No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACCTCTGTCAACAGAGTCACAGTACCCCCTCGATCAC13010.12141483276763292No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12900.1203882661569919No Hit
GTCTGCAACCTGAAGATTTTGCAACTTACCTCTGTCAACAGAGTCACAGT12870.1201082934449989No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12840.11982832073300588No Hit
GTCTGCATCTATAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA12830.1197349964956749No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT12760.11908172683435787No Hit
GTCGTGTCCCTTGGCCGAAGGTGATCGAGGGGGTACTGTGACTCTGTTGA12710.11861510564770286No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12700.11852178141037185No Hit
ATGCTGCATCCAGATTGGAAAGTGGGGTCCCATCAAGGTTCAGAGGCAGTGGATCTGGGACAGATTTCACTCTCA12670.11824180869837886No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12530.11693526937574485No Hit
CTGTCAACAGAGTCACAGTACCCCCTCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGG12420.11590870276510382No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12320.11497546039179382No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12250.11432219073047681No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12200.1138555695438218No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC11950.11152246361054675No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT11950.11152246361054675No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11820.11030924852524374No Hit
CTCCCAGGTCCAGCTTGTGCAGTCTGGGGCTGAGGTGAAGAAGTCTGGGGCCTCAGTGAAGGTTTCCTGCAAGG11820.11030924852524374No Hit
GGCCGAAGGTGATCGAGGGGGTACTGTGACTCTGTTGACAGAGGTAAGTTGCAAAATCTTCAGGTTGCAGACTG11800.11012260005058175No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC11790.11002927581325074No Hit
GCAGAAACCAGGGAAAGCCCCTAAGGTCCTGATCTATGCTGCATCCAGATTGGAAAGTGGGGTCCCATCAAGGTT11730.10946933038926472No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTATAGGAGACAGAGTCACCATCA11700.10918935767727173No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA11660.10881606072794772No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG11640.10862941225328572No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11620.1084427637786237No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG11600.10825611530396172No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT11600.10825611530396172No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11580.1080694668292997No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC11540.1076961698799757No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11440.10676292750666568No Hit
CTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCTCTGAACCT11390.10629630632001066No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA11300.10545638818403166No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT11210.10461647004805265No Hit
ATGCATTGGCTGCGCCAGGCCCCCGGACAAAGGCTTGAGTGGATGGGATG11180.10433649733605965No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC11090.10349657920008064No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11090.10349657920008064No Hit
ACTTACCTCTGTCAACAGAGTCACAGTACCCCCTCGATCACCTTCGGCCA10990.10256333682677063No Hit
GAGTCACCATCACTTGCCGGGCAAGTCAGAACATTGGCAGTTATTTAAATTGGTATCAGCAGAAACCAGGGAAAG10880.1015367702161296No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC10880.1015367702161296No Hit
ACCCAGTCTCCATCCTCCCTGTCTGCATCTATAGGAGACAGAGTCACCAT10820.10097682479214358No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC10790.10069685208015058No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC10780.10060352784281958No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC10760.10041687936815759No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGT100.009917288129.6011770
TAAGTGA150.002421707868.519331
AATGCGC150.00248224268.09399416
GTCGTAT200.007360725751.746739
CGTATGC1500.050.73352441
TATACAC1550.050.5260663
TAGCGCT1500.050.18856429
AGACTAG1550.048.4106225
TCGTATG1400.046.8760840
CGCTCAT1550.046.46430632
ATGCCGT1650.046.3234644
ACTAGCG1650.045.5746627
TTATACA1600.044.6908532
CTAGCGC1700.044.26949328
GACTAGC1700.044.20109626
CGAGACT1700.044.1143523
TACACAT1700.044.0649345
GCTGGTA10800.043.80039670
CTTATAC1650.043.344681
ATCTCGT1300.042.3586837
Produced by FastQC (version 0.11.5)