FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689048

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689048
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1071533
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT52200.4871525188678277No Hit
GTATATTACTGTGCGAGAGATCGGTACTGCAGCAGTACTACTTGCTATGATGGAGACTATTATTACTTTGACTA40920.38188277915845803No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36640.3419400055807894No Hit
GATCTATGCTGCATCCAGATTGGAAAGTGGGGTCCCATCAAGGTTCAGAGGCAGTGGATCTGGGACAGATTTCA35870.33475403930630226No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC35690.33307420303434426No Hit
GTCCTGATCTATGCTGCATCCAGATTGGAAAGTGGGGTCCCATCAAGGTT35250.3289679365917802No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG30300.2827724391129344No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30250.28230581792627946No Hit
GTAATATACAGCCGTGTCTTCAGATCTCAGGCTGCTCAGCTCCATGTAGG29580.27605309402510236No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29360.2739999608038203No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27180.25365527706566204No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26740.24954901062309792No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26250.24497612299387883No Hit
GTCTCCATCCTCCCTGTCTGCATCTATAGGAGACAGAGTCACCATCACTT26020.24282966553526583No Hit
GATCAGGACCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAT25030.23359056603949666No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24680.23032421773291162No Hit
ATAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAACATTGGCAGTTATTTAAATTGGTATCAGCAGAAA24420.2278977875623056No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23160.2161389336585994No Hit
GTCTGGGGCTGAGGTGAAGAAGTCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACAGCTTCACTAG22730.21212599145336636No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22620.21109942484272534No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22150.20671318568816827No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21720.20270024348293517No Hit
AGTCAGAACATTGGCAGTTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGGTCCTGATCTATG21250.19831400432837812No Hit
GTAATATACAGCCGTGTCTTCAGATCTCAGGCTGCTCAGCTCCATGTAGGCTGTGCTCGCGGATGTGTCGCTGGT21030.1962608711070961No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC21020.1961675468697651No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20810.19420773788581405No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20600.19224792890186304No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20500.19131468652855302No Hit
GAGTCACAGTACCCCCTCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT20410.19047476839257402No Hit
GCATAGATCAGGACCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT20390.190288119917912No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG20340.18982149873125698No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20320.189634850256595No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19620.1831021536434249No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT18420.1719032451637047No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18250.1703167331290777No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACCTCTGTCAACAGAGTCACAGTACC17990.16789030295847165No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17820.16630379092384462No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC17520.16350406380391458No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG17120.15977109431067452No Hit
CCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCA16900.15771796108939248No Hit
CCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATAACTGCCA16590.15482490973213145No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT16270.1518385341375394No Hit
GTTCAGAGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTA16250.15165188566287738No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16190.1510919402388914No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15990.14922545549227137No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15560.1452125132870383No Hit
CTGTGACTCTGTTGACAGAGGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC15380.14353267701508027No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15210.14194616498045323No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCTCTGAACCTTGATGGGACCCCA15000.13998635599650222No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14910.1391464378605232No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14800.13811987124988218No Hit
AGGGGGTACTGTGACTCTGTTGACAGAGGTAAGTTGCAAAATCTTCAGGT14510.13541346836728313No Hit
GTACCCCCTCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGA14470.13504017141795913No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC14400.1343869017566421No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14330.1337336320953251No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14160.13214712006069806No Hit
CTATTATTACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGT14130.13186714734870508No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14120.13177382311137406No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14110.1316804988740431No Hit
GTAATAATAGTCTCCATCATAGCAAGTAGTACTGCTGCAGTACCGATCTCTCGCACAGTAATATACAGCCGTGT13920.12990733836475407No Hit
GCCTACATGGAGCTGAGCAGCCTGAGATCTGAAGACACGGCTGTATATTA13900.12972068989009206No Hit
CCCCTAAGGTCCTGATCTATGCTGCATCCAGATTGGAAAGTGGGGTCCCA13660.127480908194148No Hit
GTCGTGTCCCTTGGCCGAAGGTGATCGAGGGGGTACTGTGACTCTGTTGA13590.12682763853283102No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13590.12682763853283102No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG13390.12496115378621098No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC13360.12468118107421795No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13360.12468118107421795No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13350.12458785683688697No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13120.12244139937827395No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13060.12188145395428793No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13020.12150815700496392No Hit
GTCTGCATCTATAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA12960.1209482115809779No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC12910.12048159039432291No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12700.11852178141037185No Hit
CAGTACCCCCTCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTT12700.11852178141037185No Hit
CTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCTCTGAACCT12540.11702859361307585No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA12490.11656197242642084No Hit
ATGCTGCATCCAGATTGGAAAGTGGGGTCCCATCAAGGTTCAGAGGCAGTGGATCTGGGACAGATTTCACTCTCA12440.11609535123976583No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC12230.11413554225581479No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12220.11404221801848381No Hit
CTATAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAACATTGGC12160.1134822725944978No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA11980.11180243632253976No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC11760.10974930310125772No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACCTCTGTCAACAGAGTCACAGTACCCCCTCGATCAC11630.10853608801595471No Hit
GGCCGAAGGTGATCGAGGGGGTACTGTGACTCTGTTGACAGAGGTAAGTTGCAAAATCTTCAGGTTGCAGACTG11590.10816279106663071No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT11560.10788281835463771No Hit
CCATCCTCCCTGTCTGCATCTATAGGAGACAGAGTCACCATCACTTGCCG11530.10760284564264469No Hit
CTCCCAGGTCCAGCTTGTGCAGTCTGGGGCTGAGGTGAAGAAGTCTGGGGCCTCAGTGAAGGTTTCCTGCAAGG11500.1073228729306517No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA11490.10722954869332069No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11400.10638963055734167No Hit
GTCTGCAACCTGAAGATTTTGCAACTTACCTCTGTCAACAGAGTCACAGT11390.10629630632001066No Hit
CTGTCAACAGAGTCACAGTACCCCCTCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGG11380.10620298208267967No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11200.10452314581072164No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11180.10433649733605965No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGTAATAATAGT11160.10414984886139764No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC11020.10284330953876362No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11010.10274998530143262No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10980.10247001258943962No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10980.10247001258943962No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTATAGGAGACAGAGTCACCATCA10850.1012567975041366No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10750.10032355513082658No Hit
CGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT10730.10013690665616458No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCAATC200.007772126751.0397617
CCTTACG200.007799430850.9945034
GTGGTAT2550.044.0260771
GACAGGT1050.038.8801731
GTATCAA23550.038.859541
GCATCGA350.001447809938.85848
ATAAACG350.001448474238.854773
ATGCATG1204.0713712E-738.5575670
ACAGGTA1400.036.4501651
TATCAAC25650.035.0001832
ATCAACG26400.033.8691443
TCAACGC26600.033.612924
AACGCAG27200.033.3760766
CAACGCA27050.033.1840675
GCTGGTA12300.031.70586870
ACGCAGA28900.031.4127797
CGCAGAG28750.031.3401438
AGAGTAC28900.030.82308811
ACTTAAC450.00460415830.69163140
GATTCAC2000.030.6181351
Produced by FastQC (version 0.11.5)