FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689049

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689049
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2345551
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATTGGAAGTGCCGTTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC135380.577177814509256No Hit
CCAATATACAGTGCACAGTAATAATCACAGTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCTGCTTTGTTC93880.4002471061170702No Hit
CTATTAGTCACTACCCCAACTGGTATCAGCAGACCCCAGGCCAGGCTCCA81960.349427490598158No Hit
GTAATAATCACAGTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCTG81860.3490011515417913No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC76940.32802526996854897No Hit
GATTATTACTGTGCACTGTATATTGGAAGTGCCGTTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT75460.3217154519343216No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAACGGCACTTCCAATATACAGTGCA73600.31378554548590076No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG63160.2692757480012159No Hit
CACTTAGCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCT56280.23994362092318605No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC52940.22570389644053784No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT52090.22208001446142078No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAACGGCACTTCCAATATACAGTGCACAGTAATAAT51750.220630461669774No Hit
CCCCAGGCCAGGCTCCACGCACGCTCATCTACGGCACAAGCATTCGCTCT51430.21926617668940046No Hit
GTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAGGGACAGTCACACTCACCTGTGGCTTGAGCTCTGG49500.21103783290152292No Hit
GTAATAGATGGCCGTGTCTGCGGCGGTCACAGAAATCAGCCTCAGGGAGA49270.2100572530718795No Hit
GGATGATGCTTCTCCTCGGACTCCTTTCTTATGGATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCAT49180.20967354792114945No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA48670.2074992187336792No Hit
CTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAGCAGCCCTCACCATCACGGGGGCCCAGG47650.20315056035873874No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC43200.18417847235042No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT42720.1821320448798598No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG41220.1757369590343591No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT41190.17560905731744908No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG40980.174713745299079No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA40700.1735199959412522No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG40650.17330682641306883No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG40400.17224097877215205No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG40380.1721557109608787No Hit
CCCCAGAAGAGCGAATGCTTGTGCCGTAGATGAGCGTGCGTGGAGCCTGG39880.17002401567904513No Hit
GACTAATAGAGACCGAGCCAGAGCTCAAGCCACAGGTGAGTGTGACTGTC38700.16499321481391793No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC37610.16034611909952076No Hit
GTCTCTGGTGGCTCCATCAGCGGTCACTACTGGGGCTGGATCCGGCAGCC37420.159536074892424No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG36490.15557112166821357No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG36480.1555284877625769No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG36040.15365259591456334No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT35370.1507961242369064No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC34920.14887759848325618No Hit
CCTTTCTTATGGATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGAGC34430.14678853710705927No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT34230.14593585899432585No Hit
CTACTGGGGCTGGATCCGGCAGCCCCCCGGGAGGGGACTGGAGTGGATTGGATATGTTCATCCCACTGGGAGCAC33160.14137403109120203No Hit
GATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAGGGACAGTCACA32840.14000974611082853No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC32440.1383043898853617No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA32220.13736644396135492No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA32030.13655639975425818No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA31530.1344247044724246No Hit
CTCATCTACGGCACAAGCATTCGCTCTTCTGGGGTCCCTGATCGCTTCTC31340.13361466026532784No Hit
GCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACT31090.13254881262441107No Hit
GGCCTGGATGATGCTTCTCCTCGGACTCCTTTCTTATGGATCAGGAGTGG30890.13169613451167764No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC30880.13165350060604097No Hit
CCTGTGGCTTGAGCTCTGGCTCGGTCTCTATTAGTCACTACCCCAACTGGTATCAGCAGACCCCAGGCCAGGCTC30340.12935126970166072No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT29770.12692113708037045No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA29450.12555685209999698No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC29220.12457627227035353No Hit
CACCTGTGGCTTGAGCTCTGGCTCGGTCTCTATTAGTCACTACCCCAACTGGTATCAGCAGACCCCAGGCCAGG29140.12423520102526017No Hit
GGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAGGGACAGTCACACTCAC29020.1237235941576201No Hit
CCCCTGGAGGGACAGTCACACTCACCTGTGGCTTGAGCTCTGGCTCGGTC28760.12261511261106665No Hit
CATCTACGGCACAAGCATTCGCTCTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAGCAGC28630.12206087183778992No Hit
GGCCTGGGGTCTGCTGATACCAGTTGGGGTAGTGACTAATAGAGACCGAG28290.12061131904614311No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTCCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC27440.11698743706702604No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT27430.11694480316138939No Hit
CAGTCACACTCACCTGTGGCTTGAGCTCTGGCTCGGTCTCTATTAGTCACTACCCCAACTGGTATCAGCAGACCC27200.11596422333174593No Hit
ATCTATTACTGTGCGAGACAGGAACTCATCAGGGGATATTGTAGGAGTGCCGACTGCCCAAACCGGTTCGACCC27020.11519681303028585No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC26910.11472784006828246No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG26870.11455730444573577No Hit
GTCTCTATTAGTCACTACCCCAACTGGTATCAGCAGACCCCAGGCCAGGC26740.11400306367245906No Hit
GTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCTGCTTTGTTCCCAA26520.11306511774845228No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC25980.11076288684407204No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC25790.10995284263697529No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG24960.10641422846913155No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT24720.10539101473385146No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC24520.10453833662111803No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC24190.10313141773510787No Hit
CCATCACGGGGGCCCAGGCAGATGATGACTGTGATTATTACTGTGCACTG24030.10244927524492113No Hit
TTCTCAGACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAG23830.1015965971321877No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAACGGCA23700.10104235635891097No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)