FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689050

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689050
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2345551
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATTGGAAGTGCCGTTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC126600.5397452453602587No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG102230.43584641732369067No Hit
CCAATATACAGTGCACAGTAATAATCACAGTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCTGCTTTGTTC81090.3457183408077676No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC76360.325552503441622No Hit
CTATTAGTCACTACCCCAACTGGTATCAGCAGACCCCAGGCCAGGCTCCA76040.32418821846124857No Hit
GTAATAATCACAGTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCTG73960.3153203660888209No Hit
GATTATTACTGTGCACTGTATATTGGAAGTGCCGTTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT70690.3013790789456294No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAACGGCACTTCCAATATACAGTGCA69980.29835207164542576No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG67000.2856471677656977No Hit
CACTTAGCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCT54870.23393224022841538No Hit
GTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAGGGACAGTCACACTCACCTGTGGCTTGAGCTCTGG54490.23231215181422188No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT50210.2140648402017266No Hit
GGATGATGCTTCTCCTCGGACTCCTTTCTTATGGATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCAT49480.21095256509024957No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAACGGCACTTCCAATATACAGTGCACAGTAATAAT48900.20847979856332263No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC48640.2073713170167692No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG47770.20366216722637878No Hit
CCCCAGGCCAGGCTCCACGCACGCTCATCTACGGCACAAGCATTCGCTCT46950.20016618696417177No Hit
CTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAGCAGCCCTCACCATCACGGGGGCCCAGG46250.19718181356960474No Hit
GTAATAGATGGCCGTGTCTGCGGCGGTCACAGAAATCAGCCTCAGGGAGA45810.19530592172159122No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT45210.19274788738339094No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG43790.1866938727829836No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG43420.1851164182744268No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA43290.18456217750115006No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC42050.1792755732022028No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA39340.16772178477466487No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG38820.16550482168155797No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC38590.16452424185191455No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT38390.16367156373918113No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG36740.15663696930913035No Hit
GTCTCTGGTGGCTCCATCAGCGGTCACTACTGGGGCTGGATCCGGCAGCC36390.15514478261184686No Hit
CCCCAGAAGAGCGAATGCTTGTGCCGTAGATGAGCGTGCGTGGAGCCTGG36340.1549316130836635No Hit
GACTAATAGAGACCGAGCCAGAGCTCAAGCCACAGGTGAGTGTGACTGTC35780.1525441143680099No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG35410.15096665985945307No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG35400.1509240259538164No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA35290.150455052991813No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG35090.14960237487907957No Hit
CCTTTCTTATGGATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGAGC33790.14405996714631233No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC33280.14188563795884207No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC33250.14175773624193205No Hit
GGCCTGGATGATGCTTCTCCTCGGACTCCTTTCTTATGGATCAGGAGTGG32500.1385601933191817No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA32300.1377075152064483No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT32280.13762224739517495No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC31600.1347231418118813No Hit
GATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAGGGACAGTCACA31250.13323095511459782No Hit
GCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACT31020.13225037528495437No Hit
CTCATCTACGGCACAAGCATTCGCTCTTCTGGGGTCCCTGATCGCTTCTC30350.12939390360729738No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT30090.12828542206074395No Hit
CTACTGGGGCTGGATCCGGCAGCCCCCCGGGAGGGGACTGGAGTGGATTGGATATGTTCATCCCACTGGGAGCAC30040.12807225253256058No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA29690.1265800658352771No Hit
GGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAGGGACAGTCACACTCAC29620.1262816284958204No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA29170.12436310274217016No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC29080.12397939759144012No Hit
GGCCTGGGGTCTGCTGATACCAGTTGGGGTAGTGACTAATAGAGACCGAG28840.12295618385616003No Hit
CCTGTGGCTTGAGCTCTGGCTCGGTCTCTATTAGTCACTACCCCAACTGGTATCAGCAGACCCCAGGCCAGGCTC28230.12035551561232306No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTCCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC28060.11963073921649967No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC27620.11775484736848614No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC27530.11737114221775607No Hit
CACCTGTGGCTTGAGCTCTGGCTCGGTCTCTATTAGTCACTACCCCAACTGGTATCAGCAGACCCCAGGCCAGG27360.11664636582193266No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT27130.11566578599228924No Hit
GTCTCTATTAGTCACTACCCCAACTGGTATCAGCAGACCCCAGGCCAGGC27080.11545261646410587No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC26110.11131712761734876No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC26040.11101869027789206No Hit
CATCTACGGCACAAGCATTCGCTCTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAGCAGC25650.10935596795806188No Hit
ATCTATTACTGTGCGAGACAGGAACTCATCAGGGGATATTGTAGGAGTGCCGACTGCCCAAACCGGTTCGACCC25340.10803431688332507No Hit
CCCCTGGAGGGACAGTCACACTCACCTGTGGCTTGAGCTCTGGCTCGGTC25160.10726690658186498No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG25020.1066700319029516No Hit
GTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCTGCTTTGTTCCCAA24800.10573208597894482No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG24690.10526311301694144No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA24570.10475150614930137No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC24160.10300351601819785No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAACGGCA23520.1002749460574509No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)