FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689051

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689051
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences644391
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32080.49783438936918734No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29540.458417327368011No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22150.3437354028842737No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT20680.32092316621430156No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20380.3162676077102256No Hit
CCCCTGGGGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG19960.3097498258045193No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19280.29919722652861386No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19010.2950072238749455No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18340.2846098098825092No Hit
GGTCTAGTCAGAGCCTCCTGCATGCTAATGGATACAACTTTTTGGATTGGTACCTGCAGAAGCCAGGGCAGCCTC18250.2832131423312864No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17740.2752986928743574No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17520.271884616638035No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17110.26552202001579783No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16680.25884905282662235No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16530.2565212735745844No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGAACTTATTACTGCATGCAAGCTCTAGAAAGT15740.24426163618051772No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG14870.2307605165186975No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14360.2228460670617684No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14210.22051828780973046No Hit
GGACCATACTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC14130.21927680554197684No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14120.21912162025850765No Hit
GTATGGTCCCCTGGCCAAAAGCAGGACTTTCTAGAGCTTGCATGCAGTAATAAGTTCCAACATCCTCAGCCTCCA14050.21803532327422326No Hit
ATACTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT13200.20484457417934143No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13040.2023616096438343No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA12750.19786123642322753No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT12640.19615419830506634No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGTATGGTCC12450.19320567791915158No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11560.17939418769039295No Hit
CTCCAGTATGGTCCCCTGGCCAAAAGCAGGACTTTCTAGAGCTTGCATGCAGTAATAAGTTCCAACATCCTCAGC11380.1766008525879474No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGGGAGCCGGCCTCCATCTCCT11370.17644566730447817No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11140.17287640578468663No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11070.17179010880040224No Hit
CCAGTATGGTCCCCTGGCCAAAAGCAGGACTTTCTAGAGCTTGCATGCAGTAATAAGTTCCAACATCCTCAGCCT10950.16992788539877188No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG10930.16961751483183346No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10810.1677552914302031No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10780.16728973557979548No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA10390.16123750952449678No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC10380.16108232424102759No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG10380.16108232424102759No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC10260.1592201008393972No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10090.15658195102042083No Hit
GTCAGGGACCCCGGAGGCCCGATTGGAACCCAAATAGATCAGGAGCTGTGGAGGCTGCCCTGGCTTCTGCAGGTA9990.15503009818572885No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9960.15456454233532124No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9920.15394380120144446No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGTATGGTCCCCTGGCCAAAAGCA9910.15378861591797527No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG9900.15363343063450607No Hit
GTAATAAGTTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA9830.15254713365022168No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9800.1520815777998141No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9630.1494434279808377No Hit
CAGTAATAAGTTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA9560.14835713099655334No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9530.14789157514614573No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9370.14540861061063856No Hit
CCATACTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT9360.14525342532716937No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9310.1444774989098234No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT9270.1438567577759466No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA9250.1435463872090082No Hit
AGCCAGGGCAGCCTCCACAGCTCCTGATCTATTTGGGTTCCAATCGGGCC9230.14323601664206978No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT8940.13873564342146305No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8890.13795971700411705No Hit
AGTCAGAGCCTCCTGCATGCTAATGGATACAACTTTTTGGATTGGTACCTGCAGAAGCCAGGGCAGCCTCCACAG8880.13780453172064785No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA8830.13702860530330188No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACT8610.13361452906697951No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC8570.13299378793310274No Hit
GAGTGGAGGCTGAGGATGTTGGAACTTATTACTGCATGCAAGCTCTAGAA8440.13097637924800315No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA8440.13097637924800315No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8230.12771748829515No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC8160.12663119131086562No Hit
GCTCTAGAAAGTCCTGCTTTTGGCCAGGGGACCATACTGGAGATCAAACG8100.12570007961005042No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC8080.12538970904311203No Hit
CTCCCTGCCCGTCACCCCTGGGGAGCCGGCCTCCATCTCCTGCAGGTCTA8050.12492415319270442No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8040.12476896790923524No Hit
GTCCTGCTTTTGGCCAGGGGACCATACTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGC7970.12368267092495085No Hit
ACTTATTACTGCATGCAAGCTCTAGAAAGTCCTGCTTTTGGCCAGGGGAC7940.12321711507454325No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA7900.12259637394066647No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG7870.12213081809025886No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG7840.12166526223985126No Hit
CCCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAATATCC7820.12135489167291287No Hit
GATTCACTTTATTTATTCATTCTCCTCCAACATTAGCATAATTAAAGCCA7620.11825118600352891No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA7620.11825118600352891No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC7500.11638896260189853No Hit
GGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCCCTGGGGAGCCGGCCTCCATCTCCTGCAGGT7390.11468192448373736No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC7390.11468192448373736No Hit
GCAGAAGCCAGGGCAGCCTCCACAGCTCCTGATCTATTTGGGTTCCAATCGGGCCTCCGGGGTCCCTGACAGGTT7340.11390599806639137No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT7130.1106471071135382No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC7080.10987118069619223No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA7060.10956081012925382No Hit
CCACTCTCCCTGCCCGTCACCCCTGGGGAGCCGGCCTCCATCTCCTGCAG7040.10925043956231542No Hit
GACTAGACCTGCAGGAGATGGAGGCCGGCTCCCCAGGGGTGACGGGCAGG6950.10785377201109264No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC6930.10754340144415425No Hit
CTAGAAAGTCCTGCTTTTGGCCAGGGGACCATACTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCAT6920.10738821616068504No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA6920.10738821616068504No Hit
TTATTACTGCATGCAAGCTCTAGAAAGTCCTGCTTTTGGCCAGGGGACCATACTGGAGATCAAACGAACTGTGG6830.10599154860946228No Hit
GGCACAGATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGG6740.1045948810582395No Hit
GTGGAGGCTGAGGATGTTGGAACTTATTACTGCATGCAAGCTCTAGAAAGTCCTGCTTTTGGCCAGGGGACCATA6710.1041293252078319No Hit
GCCCTGGCTTCTGCAGGTACCAATCCAAAAAGTTGTATCCATTAGCATGC6700.10397413992436269No Hit
TTCTAGAGCTTGCATGCAGTAATAAGTTCCAACATCCTCAGCCTCCACTC6700.10397413992436269No Hit
CCACAGCTCCTGATCTATTTGGGTTCCAATCGGGCCTCCGGGGTCCCTGA6660.1033533987904859No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT6640.1030430282235475No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC6590.10226710180620152No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA6560.10180154595579391No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT6530.10133599010538631No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG6490.10071524897150953No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT6480.10056006368804034No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAA300.002621965367.14970
CCGCATA250.00603558554.4053669
GCGCCAT200.00692142552.55567645
TCTAGCG200.00825344550.26743728
CCCGAAT200.00843307349.99437310
CCTAACG706.202754E-1042.8523182
CTAACGC751.2205419E-939.9955023
TAGTGAT858.381484E-539.49941370
CTCTTAT1100.039.3956221
CGTATGC2950.038.6610541
ATCTCGT2850.038.25694337
ATGCCGT3050.037.80722844
TAGTACC451.2265978E-437.032874
TCGTATG3100.036.6605540
TATCTCG3050.035.705836
GGTATCA13650.035.1663361
CGAGACG3150.034.9546823
ATCTCCG3150.034.91670610
TGCTTGA3450.034.63550655
CGGACTC3300.034.5271328
Produced by FastQC (version 0.11.5)