FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689052

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689052
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences644391
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38720.6008774175927348No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31120.48293660215614437No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24550.38097987091688124No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23720.36809949238893774No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19950.3095946405210501No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18790.29159314763862315No Hit
GGTCTAGTCAGAGCCTCCTGCATGCTAATGGATACAACTTTTTGGATTGGTACCTGCAGAAGCCAGGGCAGCCTC18390.2853857362998552No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17940.2784023985437413No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17580.27281572833885015No Hit
CCCCTGGGGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG16710.25931460867702993No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16700.25915942339356074No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16510.256210903007646No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15660.24302015391276413No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15260.23681274257399623No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGAACTTATTACTGCATGCAAGCTCTAGAAAGT15050.23355385162114306No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14770.2292086636840055No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14680.22781199613278275No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14340.22253569649483002No Hit
GTATGGTCCCCTGGCCAAAAGCAGGACTTTCTAGAGCTTGCATGCAGTAATAAGTTCCAACATCCTCAGCCTCCA13880.2153971734552469No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13660.21198309721892455No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13480.209189762116479No Hit
GGACCATACTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC13280.206086056447095No Hit
ATACTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT13080.20298235077771104No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG12900.20018901567526548No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG12750.19786123642322753No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12660.19646456887200472No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT12410.19258493678527477No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12300.1908778986671136No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGTATGGTCC11970.18575678431263007No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA11940.18529122846222246No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGGGAGCCGGCCTCCATCTCCT11790.1829634492101845No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11760.1824978933597769No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC10480.16263417707571956No Hit
CTCCAGTATGGTCCCCTGGCCAAAAGCAGGACTTTCTAGAGCTTGCATGCAGTAATAAGTTCCAACATCCTCAGC10410.16154788009143517No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG10390.16123750952449678No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA10130.15720269215429764No Hit
GTAATAAGTTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA10100.15673713630389002No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGTATGGTCCCCTGGCCAAAAGCA10030.15565083931960566No Hit
GTCAGGGACCCCGGAGGCCCGATTGGAACCCAAATAGATCAGGAGCTGTGGAGGCTGCCCTGGCTTCTGCAGGTA10000.15518528346919805No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9910.15378861591797527No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9820.15239194836675246No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG9780.1517712072328757No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9710.1506849102485913No Hit
CTCCCTGCCCGTCACCCCTGGGGAGCCGGCCTCCATCTCCTGCAGGTCTA9700.1505297249651221No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC9390.14571898117757698No Hit
CCAGTATGGTCCCCTGGCCAAAAGCAGGACTTTCTAGAGCTTGCATGCAGTAATAAGTTCCAACATCCTCAGCCT9360.14525342532716937No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9270.1438567577759466No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA9230.14323601664206978No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9150.14199453437431622No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9130.1416841638073778No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9110.14137379324043942No Hit
AGCCAGGGCAGCCTCCACAGCTCCTGATCTATTTGGGTTCCAATCGGGCC9060.14059786682309344No Hit
CAGTAATAAGTTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA8990.13951156983880902No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT8980.13935638455533986No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8850.13733897587024027No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC8720.1353215671851407No Hit
CCATACTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT8710.13516638190167152No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT8660.13439045548432552No Hit
GAGTGGAGGCTGAGGATGTTGGAACTTATTACTGCATGCAAGCTCTAGAA8620.13376971435044874No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA8480.13159712038187996No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC8420.13066600868106476No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACT8380.13004526754718798No Hit
AGTCAGAGCCTCCTGCATGCTAATGGATACAACTTTTTGGATTGGTACCTGCAGAAGCCAGGGCAGCCTCCACAG8360.12973489698024956No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8260.1281830441455576No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA8210.1274071177282116No Hit
GGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCCCTGGGGAGCCGGCCTCCATCTCCTGCAGGT8160.12663119131086562No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG8160.12663119131086562No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA8130.12616563546045803No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC8080.12538970904311203No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC8020.12445859734229683No Hit
GTCCTGCTTTTGGCCAGGGGACCATACTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGC7960.12352748564148164No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT7850.12182044752332047No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA7840.12166526223985126No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA7840.12166526223985126No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT7810.12119970638944369No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC7610.11809600072005971No Hit
GCTCTAGAAAGTCCTGCTTTTGGCCAGGGGACCATACTGGAGATCAAACG7510.11654414788536774No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC7420.11514748033414496No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG7410.11499229505067575No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA7360.11421636863332976No Hit
ACTTATTACTGCATGCAAGCTCTAGAAAGTCCTGCTTTTGGCCAGGGGAC7350.11406118334986055No Hit
TTCTAGAGCTTGCATGCAGTAATAAGTTCCAACATCCTCAGCCTCCACTC7330.11375081278292218No Hit
GTGGAGGCTGAGGATGTTGGAACTTATTACTGCATGCAAGCTCTAGAAAGTCCTGCTTTTGGCCAGGGGACCATA7120.110491921830069No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC7060.10956081012925382No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT6940.10769858672762345No Hit
GACTAGACCTGCAGGAGATGGAGGCCGGCTCCCCAGGGGTGACGGGCAGG6940.10769858672762345No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT6850.10630191917640067No Hit
GCAGAAGCCAGGGCAGCCTCCACAGCTCCTGATCTATTTGGGTTCCAATCGGGCCTCCGGGGTCCCTGACAGGTT6840.10614673389293147No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG6820.10583636332599307No Hit
CCCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAATATCC6730.10443969577477029No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA6710.1041293252078319No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC6540.10149117538885552No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)