FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689053

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689053
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1238066
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCTATAGTGTATCAAACAGGGCCACTGGCGTCCCAGACAGGTTCAGTGGCAGTGGGTCCGGGACAGACTTCA49310.3982824825170871No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT49200.3973939999967691No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC47040.3799474341432525No Hit
CTATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTGTGCTGGTACCAGGC42220.3410157455256828No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTC37970.3066880117861245No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34300.27704500406278826No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31390.25354060284346713No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG29040.2345593853639467No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28670.23157085325014984No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28290.22850154999814226No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27960.22583610243718832No Hit
CCCTAGAGCCTGAAGATTCTGCAATTTATTACTGTCAACACCGTAGCAAC26580.2146896853641082No Hit
GTACTTAGCCTGGTACCAGCACAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATAGTGTATCAAACAGGGCCA26470.21380120284379026No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26430.21347811829094732No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25250.20394712398208176No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25240.203866352843871No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24600.19869699999838455No Hit
GTCTCCAGCCACCCTGTCTCTGTCTCCAGGGGAACGAGCCACCCTCTCCT23520.18997371707162625No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT23350.1886006077220439No Hit
GTATCAAACAGGGCCACTGGCGTCCCAGACAGGTTCAGTGGCAGTGGGTC23340.18851983658383317No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23190.18730826951067228No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT21610.17454642967337766No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21530.17390026056769187No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCC21250.17163866869779157No Hit
GTTTGATACACTATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTGTGCT21150.17083095731568432No Hit
GAGTGTTACCAAGTACTTAGCCTGGTACCAGCACAAACCTGGCCAGGCTC21050.17002324593357704No Hit
GTCCGGGACAGACTTCACTCTCAGCATCAGCACCCTAGAGCCTGAAGATT20030.16178458983608304No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTACCAAGTACTTAGCCTGGTACCAGCACAAACCTGGCCAGGCTCCCAGGCT19600.15831143089302185No Hit
GAGTGAAGTCTGTCCCGGACCCACTGCCACTGAACCTGTCTGGGACGCCA19360.15637292357596444No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19260.1555652121938572No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19120.15443441625890705No Hit
GACTTCACTCTCAGCATCAGCACCCTAGAGCCTGAAGATTCTGCAATTTATTACTGTCAACACCGTAGCAACTGG19070.15403056056785341No Hit
GTCAGAGTGTTACCAAGTACTTAGCCTGGTACCAGCACAAACCTGGCCAG18460.14910352113699915No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18280.1476496406492061No Hit
GTCTCTGTCTCCAGGGGAACGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCAAGTACTTAGCCTGGTA17830.14401493942972346No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17830.14401493942972346No Hit
GGTTTGTGCTGGTACCAGGCTAAGTACTTGGTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCGTTCCC17750.14336877032403764No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTG16080.12987999024284652No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16050.12963767682821434No Hit
GCACAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATAGTGTATCAAACAGGGCCACTGGCGTCCCAGACAGGTT15930.12866842316968563No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15630.12624528902336385No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14960.12083362276324526No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14850.11994514024292727No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14790.11946051341366291No Hit
GTCCCGGACCCACTGCCACTGAACCTGTCTGGGACGCCAGTGGCCCTGTT14660.11841048861692348No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14490.11703737926734116No Hit
CCCTGTCTCTGTCTCCAGGGGAACGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCAAGTACTTAGC14470.1168758369909197No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14450.11671429471449826No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14430.11655275243807682No Hit
ACCAAGTACTTAGCCTGGTACCAGCACAAACCTGGCCAGGCTCCCAGGCT14140.11421038942996578No Hit
ATTTATTACTGTCAACACCGTAGCAACTGGCCCCTATTCACTTTCGGCCC14130.11412961829175504No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG14110.1139680760153336No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA14050.11348344918606923No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13920.11243342438932982No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13910.11235265325111908No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13860.11194879756006544No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13670.11041414593406167No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13610.10992951910479731No Hit
CCGTAGCAACTGGCCCCTATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCAT13600.1098487479665866No Hit
CTGCTACTCTGGCTCCCAGTTTCAGAAACCACCGGAGAAATTGTGTTGACACAGTCTCCAGCCACCCTGTCT13300.10742561382026483No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13250.10702175812921122No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTCTGTCTCCAGGGGAACGAGC13140.10613327560889323No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT13120.10597173333247178No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA12930.104437081706468No Hit
GTGTTACCAAGTACTTAGCCTGGTACCAGCACAAACCTGGCCAGGCTCCC12720.10274088780404275No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCC12710.10266011666583204No Hit
GTAATAAATTGCAGAATCTTCAGGCTCTAGGGTGCTGATGCTGAGAGTGAAGTCTGTCCCGGACCCACTGCCA12540.1012870073162497No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG12410.10023698251951027No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTAT451.8964674E-645.87584739
GGTATCA15550.045.874581
CGTATGC5950.045.26225341
TCGTATG5500.044.49080740
TCGACGT7350.044.48813629
CGACGTC7450.044.3787430
ACTCGAC7300.044.28527
ATGCCGT6000.043.93261344
CTCGACG7400.043.7077828
ATCTCCG7700.043.22109210
CTTATAC8100.043.1951941
GTCGTAT803.6379788E-1143.0086139
ACGAGAC8000.042.8893722
GCGTATG651.1488737E-842.418140
GACTCGA7650.042.24350426
CGAGACT8000.042.05536723
CACGAGA8000.042.04007721
ATCTCGT4500.041.9584337
AGACTCG7950.041.9178125
CCCACGA8150.041.67137519
Produced by FastQC (version 0.11.5)