FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689054

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689054
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1238066
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG76800.6203223414583714No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT49770.4019979548747805No Hit
CATCTATAGTGTATCAAACAGGGCCACTGGCGTCCCAGACAGGTTCAGTGGCAGTGGGTCCGGGACAGACTTCA46370.37453576788313386No Hit
CTATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTGTGCTGGTACCAGGC40080.32373072194858754No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36090.29150303780250814No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTC35910.2900491573147151No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33360.26945251707098006No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33280.26880634796529423No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29340.23698251951026844No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27450.2217167743884413No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25420.20532023333166408No Hit
CCCTAGAGCCTGAAGATTCTGCAATTTATTACTGTCAACACCGTAGCAAC25380.20499714877882116No Hit
GTACTTAGCCTGGTACCAGCACAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATAGTGTATCAAACAGGGCCA25150.20313941259997448No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25090.2026547857707101No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24960.2016047609739707No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23690.19134682642120857No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23480.18965063251878334No Hit
GTATCAAACAGGGCCACTGGCGTCCCAGACAGGTTCAGTGGCAGTGGGTC22380.18076580731560352No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21870.17664647926685653No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20940.16913476341325906No Hit
GAGTGTTACCAAGTACTTAGCCTGGTACCAGCACAAACCTGGCCAGGCTC20630.16663085812872658No Hit
GTTTGATACACTATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTGTGCT20360.164450037397037No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTACCAAGTACTTAGCCTGGTACCAGCACAAACCTGGCCAGGCTCCCAGGCT19880.16057302276292218No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCC19870.16049225162471145No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT19810.16000762479544708No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19700.1591191422751291No Hit
GAGTGAAGTCTGTCCCGGACCCACTGCCACTGAACCTGTCTGGGACGCCA19670.15887682886049692No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19440.15701909268165024No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19410.15677677926701805No Hit
GTCCGGGACAGACTTCACTCTCAGCATCAGCACCCTAGAGCCTGAAGATT19090.15419210284427484No Hit
GACTTCACTCTCAGCATCAGCACCCTAGAGCCTGAAGATTCTGCAATTTATTACTGTCAACACCGTAGCAACTGG19080.15411133170606414No Hit
GTCTCCAGCCACCCTGTCTCTGTCTCCAGGGGAACGAGCCACCCTCTCCT18940.15298053577111398No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18040.1457111333321487No Hit
GGTTTGTGCTGGTACCAGGCTAAGTACTTGGTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCGTTCCC17870.14433802398256637No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17620.14231874552729823No Hit
GTCTCTGTCTCCAGGGGAACGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCAAGTACTTAGCCTGGTA17440.14086486503950515No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16740.13521088536475437No Hit
GTCAGAGTGTTACCAAGTACTTAGCCTGGTACCAGCACAAACCTGGCCAG16290.13157618414527172No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15960.12891073658431781No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTG15850.1280222540639998No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15290.1234990703241992No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15070.12172210528356324No Hit
GTCCCGGACCCACTGCCACTGAACCTGTCTGGGACGCCAGTGGCCCTGTT14590.11784509064944843No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14530.11736046382018406No Hit
CTGCTACTCTGGCTCCCAGTTTCAGAAACCACCGGAGAAATTGTGTTGACACAGTCTCCAGCCACCCTGTCT14520.11727969268197334No Hit
GCACAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATAGTGTATCAAACAGGGCCACTGGCGTCCCAGACAGGTT14420.11647198129986608No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13990.11299882235680488No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13840.111787255283644No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13640.1101718325194295No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13500.10904103658447935No Hit
ATTTATTACTGTCAACACCGTAGCAACTGGCCCCTATTCACTTTCGGCCC13310.10750638495847556No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13250.10702175812921122No Hit
ACCAAGTACTTAGCCTGGTACCAGCACAAACCTGGCCAGGCTCCCAGGCT13240.10694098699100048No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13170.1063755890235254No Hit
CCCTGTCTCTGTCTCCAGGGGAACGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCAAGTACTTAGC13150.10621404674710395No Hit
GTGTTACCAAGTACTTAGCCTGGTACCAGCACAAACCTGGCCAGGCTCCC13070.10556787764141815No Hit
GTAATAAATTGCAGAATCTTCAGGCTCTAGGGTGCTGATGCTGAGAGTGAAGTCTGTCCCGGACCCACTGCCA13020.10516402195036452No Hit
CCGTAGCAACTGGCCCCTATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCAT12880.10403322601541436No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12770.10314474349509639No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTCTGTCTCCAGGGGAACGAGC12680.10241780325119984No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12600.10177163414551406No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG12480.10080238048698535No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)