FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689055

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689055
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences664365
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23240.34980771112265097No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22070.3321969098311922No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15860.23872419528421876No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15340.2308971724880149No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14990.22562898406749302No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14940.22487638572170418No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT14230.21418948921150274No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13560.20410467137793234No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12980.1953745305667818No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12390.18649387008647353No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11680.1758069735762721No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11480.17279658019311675No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT11050.16632423441933275No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10490.15789513294649776No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10370.15608889691660457No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10290.15488473956334242No Hit
GTTTATTACTGTCAGCAGCGTACCAACTGGCCTCCGGGGGTCACCTTCGGCCAAGGGACACGACTGGATATTAAA10150.1527774641951337No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA10140.15262694452597594No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10040.15112174783439825No Hit
GTCTGGGACAGACTTCACTCTCATCATCAGTAGCCTAGAGCCTGAAGATT9730.14645563809050746No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGC9710.14615459875219194No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9690.14585355941387643No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9240.13908017430177688No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT8900.1339625055504128No Hit
GGACACGACTGGATATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC8610.12959743514483754No Hit
GACTTCACTCTCATCATCAGTAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTACCAACTGG8590.129296395806522No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG8540.1285437974607332No Hit
GAATAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCATCATCAGTAG8520.12824275812241764No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC8420.12673756143083997No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG8170.1229745697018958No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT8130.12237249102526472No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8110.1220714516869492No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7790.11725482227390063No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG7740.11650222392811181No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTGACCTGCAGGGCCAGTCAGAGTGTTGGCAACTACTTAGCCTGGTA7700.11590014525148074No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7610.11454546822906084No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA7530.1133413108757987No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC7500.1128897518683254No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG7470.11243819286085209No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7380.11108351583843218No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT7280.10957831914685451No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG7250.1091267601393812No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG7100.10686896510201471No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC7090.10671844543285694No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT7040.1059658470870681No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTGACCTGCAGGGCCAGTCAGAGTGTTGGCAA7020.10566480774875256No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT6940.10446065039549043No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG6940.10446065039549043No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC6790.10220285535812391No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG150.002400888468.6657336
CGGGTAT200.007748570351.0768246
CTAACGC200.007748570351.0768243
GGTATCA9600.045.4118771
GCCGTCT3400.042.9786746
TCGTATG3250.042.48797240
ATCTCCG3900.041.9091910
ATCTCGT3200.041.884537
CTTATAC4000.041.722161
ACGAGAC4050.041.2070722
CGTATGC3450.041.0913741
AGCCTAT3700.040.7108832
CCCACGA4100.040.6984219
GTATCAA25650.039.8349761
GAGACCG4200.039.7623324
CACGAGA4200.039.732421
GCCTATC3800.039.66054533
CGGAGCC4150.039.50047729
TCTCCGA4150.039.38453711
GTATGCC3700.039.31252742
Produced by FastQC (version 0.11.5)