FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689063

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689063
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences948473
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAAGACGGTCAGCTTGGTCCCTCCGCCGAAGAGCCA42040.44323876378136223No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTCGAGCCCAG40680.42889992651345904No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC37500.39537235113703817No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG30360.32009345548054613No Hit
CTCCTGCACTGGGACCAGCTCCAACATCGGGGCAGGTTATGAAGTACACTGGTACCAGCAGGTTCCAGGAACAGC27790.29299727034928774No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT25370.2674825746225776No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAAGACGGTCAGCTTGGTCCCTC25230.2660065178449993No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA23430.24702864499042146No Hit
GACCTAAGACGGTCAGCTTGGTCCCTCCGCCGAAGAGCCAAACCCCAATC21040.2218302471446209No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT20710.21835097045461493No Hit
GTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC20310.21413366537581988No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT20270.21371193486794038No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGCGGCCTGGGCCCAGG19980.21065438868581393No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG19720.20791314038459716No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC19240.20285237429004305No Hit
GTCCTATGACAGCGGCGTGATTGGGGTTTGGCTCTTCGGCGGAGGGACCA18740.19758074294154918No Hit
GGCCATCACTGGGCTCGAGGCTGAGGATGAGGCTGATTATTACTGCCAGT18550.19557752302912157No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18530.19536665777518178No Hit
GGTTATGAAGTACACTGGTACCAGCAGGTTCCAGGAACAGCCCCCAAACTCCTCATCTATGGTAACACCAATCGG18440.1944177641324529No Hit
GATTATTACTGCCAGTCCTATGACAGCGGCGTGATTGGGGTTTGGCTCTTCGGCGGAGGGACCAAGCTGACCGT18420.19420689887851314No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG17930.18904070015698918No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17650.18608858660183264No Hit
GTCTTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC17630.18587772134789288No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17610.18566685609395311No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT17350.1829256077927363No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA17160.18092238788030868No Hit
ATGGTAACACCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGG16950.17870830271394125No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16580.1748072955160558No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG16540.1743855650081763No Hit
TCTTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG16140.17016825992938123No Hit
GGGCAGAGGATCACCATCTCCTGCACTGGGACCAGCTCCAACATCGGGGCAGGTTATGAAGTACACTGGTACCAG16100.1697465294215017No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG15990.1685867705248331No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15300.16131191926391156No Hit
GTGTTACCATAGATGAGGAGTTTGGGGGCTGTTCCTGGAACCTGCTGGTA14630.15424793325692984No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA14610.15403706800299008No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAAGACGGTCAGCTTGGTCCCTCCGCCGAAGAG14530.15319360698723106No Hit
GTAATAATCAGCCTCATCCTCAGCCTCGAGCCCAGTGATGGCCAGGGAGG14320.15097952182086366No Hit
GTAATACATGGCCGTGTCCGCGGCGGTCACAGAGCTCAGTTCCAGGGAGA14040.1480274082657071No Hit
GAAGTACACTGGTACCAGCAGGTTCCAGGAACAGCCCCCAAACTCCTCAT13830.1458133230993397No Hit
GGATGAGGCTGATTATTACTGCCAGTCCTATGACAGCGGCGTGATTGGGG13820.1457078904723698No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC13720.14465356420267103No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC13460.14191231590145426No Hit
GCCTCATCCTCAGCCTCGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCC13140.13853847183841816No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13080.1379058760765989No Hit
CTTCATAACCTGCCCCGATGTTGGAGCTGGTCCCAGTGCAGGAGATGGTGATCCTCTGCCCTGGGGCCCCAGACA13000.1370624150608399No Hit
GTGTCTGGGGCCCCAGGGCAGAGGATCACCATCTCCTGCACTGGGACCAG12730.13421573413265322No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12360.13031472693476778No Hit
GTACACTGGTACCAGCAGGTTCCAGGAACAGCCCCCAAACTCCTCATCTA12250.12915496803809912No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC12150.12810064176840036No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12140.1279952091414305No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAAGACGGTCAGCTTGGTC12080.12736261337961124No Hit
GTGCAGGAGATGGTGATCCTCTGCCCTGGGGCCCCAGACACTGAGGGCGG12080.12736261337961124No Hit
GTTATGAAGTACACTGGTACCAGCAGGTTCCAGGAACAGCCCCCAAACTCCTCATCTATGGTAACACCAATCGGC11720.12356703880869566No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGCGG11150.11755737907141268No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCACTGGGCTCGAGGCTGAGGATG10940.11534329390504526No Hit
GTCTCTGGTGGCTCCGTCACCAGTGATGGTTTCTCCTGGAGTTGGATCCG10900.11492156339716576No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT10780.11365637187352724No Hit
GCCCCCAAACTCCTCATCTATGGTAACACCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT10650.11228574772291884No Hit
GCTTGGTCCCTCCGCCGAAGAGCCAAACCCCAATCACGCCGCTGTCATAGGACTGGCAGTAATAATCAGCCTCAT10610.11186401721503933No Hit
GCTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCT10600.11175858458806946No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC10550.11123142145322007No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10470.11038796043746106No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG10410.1097553646756418No Hit
CCCTCAGTGTCTGGGGCCCCAGGGCAGAGGATCACCATCTCCTGCACTGG10340.10901733628685265No Hit
TGGTAACACCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGC10320.1088064710329129No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10320.1088064710329129No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA10280.10838474052503339No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT10270.10827930789806353No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC10230.10785757739018402No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10160.10711954900139488No Hit
GTATTACTGTGCCAGAATGTATTTCTTTGATACTAGTGGTTCCGGCCCGC10100.10648695323957562No Hit
GTTTGGGGGCTGTTCCTGGAACCTGCTGGTACCAGTGTACTTCATAACCT10070.10617065535866597No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG10040.10585435747775636No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG9760.10290224392259979No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC9670.10195335027987092No Hit
CAGTAATACATGGCCGTGTCCGCGGCGGTCACAGAGCTCAGTTCCAGGGA9600.10121532189108176No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGCGGCCTGGGC9560.10079359138320226No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG4100.062.19457270
CGTATGC3900.052.23876641
CGACGAA200.00746404451.5641338
TTAACGG200.00756205851.39426835
TCGTATG3900.051.2847140
ATCTCGT3750.051.27482637
GATCTCG3700.051.0017136
CTTATAC4150.049.9877621
ACATGCG4100.049.83623527
TCTCGTA3800.049.7548638
ATGCGCA4250.049.72190529
CTTGAAA4200.048.99171457
TATACAC4400.048.6754043
TTATACA4300.048.2262042
CGCAGAT4300.047.67184432
TGCGCAG4300.047.59619530
GCCGTCT4350.047.193646
AGATCTC4250.046.7587135
TGCTTGA4550.046.5622455
GCGCAGA4400.046.551431
Produced by FastQC (version 0.11.5)